26 documents found, page 1 of 3
Ferritin heavy chain supports stability and function of the regulatory T cell l...
Wu, Qian; Carlos, Ana Rita; Braza, Faouzi; Bergman, Marie-Louise; Kitoko, Jamil Z; Bastos-Amador, Patricia; Cuadrado, Eloy; Martins, RuiRegulatory T (TREG) cells develop via a program orchestrated by the transcription factor forkhead box protein P3 (FOXP3). Maintenance of the TREG cell lineage relies on sustained FOXP3 transcription via a mechanism involving demethylation of cytosine-phosphate-guanine (CpG)-rich elements at conserved non-coding sequences (CNS) in the FOXP3 locus. This cytosine demethylation is catalyzed by the ten–eleven transl...
The Rare DRB1*04:08-DQ8 Haplotype is the Main HLA Class II Genetic Driver and D...
Caramalho, Iris; Matoso, Paula; Ligeiro, Dário; Paixão, Tiago; Sobral, Daniel; Fitas, Ana Laura; Limbert, Catarina; Demengeot, JocelyneIntroduction: Early-onset Type 1 diabetes (EOT1D) is considered a disease subtype with distinctive immunological and clinical features. While both Human Leukocyte Antigen (HLA) and non-HLA variants contribute to age at T1D diagnosis, detailed analyses of EOT1D-specific genetic determinants are still lacking. This study scrutinized the involvement of the HLA class II locus in EOT1D genetic control. Methods: We c...
Clinical features related to severity and mortality among COVID-19 patients in ...
Sebastião, Cruz S.; Cogle, Adis; Teixeira, Alice D’Alva; Cândido, Ana Micolo; Tchoni, Chissengo; Amorim, Maria João; Loureiro, N’guezaBackground: Infection due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with clinical features of diverse severity. Few studies investigated the severity and mortality predictors of coronavirus disease 2019 (COVID-19) in Africa. Herein, we investigated the clinical features of severity and mortality among COVID-19 patients in Luanda, Angola. Methods: This multicenter cohort study...
Clinical features related to severity and mortality among COVID-19 patients in ...
Sebastião, Cruz S.; Cogle, Adis; Teixeira, Alice D’Alva; Cândido, Ana Micolo; Tchoni, Chissengo; Amorim, Maria João; Loureiro, N’guezaBackground: Infection due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with clinical features of diverse severity. Few studies investigated the severity and mortality predictors of coronavirus disease 2019 (COVID-19) in Africa. Herein, we investigated the clinical features of severity and mortality among COVID-19 patients in Luanda, Angola. Methods: This multicenter cohort study...
Specific Eco-evolutionary Contexts in the Mouse Gut Reveal Escherichia coli Met...
Barroso-Batista, João; Pedro, Miguel F; Sales-Dias, Joana; Pinto, Catarina J G; Thompson, Jessica A; Pereira, Helena; Demengeot, Jocelyne; Gordo, IsabelMembers of the gut microbiota are thought to experience strong competition for nutrients. However, how such competition shapes their evolutionary dynamics and depends on intra- and interspecies interactions is poorly understood. Here, we test the hypothesis that Escherichia coli evolution in the mouse gut is more predictable across hosts in the absence of interspecies competition than in the presence of other m...
Route of Antigen Presentation Can Determine the Selection of Foxp3-Dependent or...
Agua-Doce, Ana; Caridade, Marta; Oliveira, Vanessa G; Bergman, Lisa; Lafaille, Maria C; Lafaille, Juan J; Demengeot, Jocelyne; Graca, LuisIt has been shown that dominant tolerance, namely in transplantation, requires Foxp3+regulatory T cells. Although most tolerance-inducing regimens rely on regulatory T cells, we found that induction of tolerance to proteins in aluminum hydroxide can be achieved in Foxp3-deficient mice using nondepleting anti-CD4 Abs. This type of tolerance is Ag specific, and tolerant mice retain immune competence to respond to...
iRAGu: A Novel Inducible and Reversible Mouse Model for Ubiquitous Recombinase ...
Bonnet, Marie; Sarmento, Leonor Morais; Martins, Ana C.; Sobral, Daniel; Silva, Joana; Demengeot, JocelyneDeveloping lymphocytes express the recombination activating genes (RAGs) 1 and 2 products that form a site specific recombinase complex (RAG), introducing double strand DNA breaks (DSBs) at recombination signal sequences (RSSs) flanking the V, D, and J gene segments in the antigen receptor loci. The subsequent steps in the reaction consist in the ligation of DSBs by ubiquitous enzymes of the non-homologous end ...
Tolerogenic insulin peptide therapy precipitates type 1 diabetes
Bergman, Marie-Louise; Lopes-Carvalho, Thiago; Martins, Ana-Catarina; Grieco, Fabio A.; Eizirik, Décio L.; Demengeot, JocelyneDaniel et al. (https://doi.org/10.1084/jem.20110574) have previously published in JEM a study on the preventive effect of tolerogenic vaccination with a strong agonist insulin mimetope in type 1 diabetes. Our study now challenges these results and shows that osmotic pump delivery of the modified insulin peptide R22E did not prevent hyperglycemia, accelerated disease onset, increased its incidence, and worsened ...
Tolerogenic insulin peptide therapy precipitates type 1 diabetes
Bergman, Marie-Louise; Lopes-Carvalho, Thiago; Martins, Ana-Catarina; Grieco, Fabio A.; Eizirik, Décio L.; Demengeot, JocelyneDaniel et al. (https://doi.org/10.1084/jem.20110574) have previously published in JEM a study on the preventive effect of tolerogenic vaccination with a strong agonist insulin mimetope in type 1 diabetes. Our study now challenges these results and shows that osmotic pump delivery of the modified insulin peptide R22E did not prevent hyperglycemia, accelerated disease onset, increased its incidence, and worsened ...
Myocardial aging as a T-cell–mediated phenomenon
Ramos, Gustavo Campos; van den Berg, Anne; Nunes-Silva, Vânia; Weirather, Johannes; Peters, Laura; Burkard, Matthias; Friedrich, Mike; Pinnecker, JürgenIn recent years, the myocardium has been rediscovered under the lenses of immunology, and lymphocytes have been implicated in the pathogenesis of cardiomyopathies with different etiologies. Aging is an important risk factor for heart diseases, and it also has impact on the immune system. Thus, we sought to determine whether immunological activity would influence myocardial structure and function in elderly mice...