45 documents found, page 1 of 5
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substra...
Becerra-Rodríguez, Carmen; Taghouti, Géraldine; Portier, Perrine; Dequin, Sylvie; Casal, Margarida; Paiva, Sandra; Galeote, VirginieFungal Oligopeptide Transporters (Fot) Fot1, Fot2 and Fot3 have been found in <i>Saccharomyces cerevisiae</i> wine strains, but not in strains from other environments. In the <i>S. cerevisiae</i> wine strain EC1118, Fot1 and Fot2 are responsible for a broader range of oligopeptide utilization in comparison with strains not containing any Fot. This leads to better fermentation efficiency and an increased product...
Integrating transcriptomics and metabolomics for the analysis of the aroma prof...
Mendes, Inês Isabel Moreira Moutinho Vieira; Sanchez, Isabelle; Franco-Duarte, Ricardo; Camarasa, Carole; Schuller, Dorit Elisabeth; Dequin, SylvieBackground: During must fermentation thousands of volatile aroma compounds are formed, with higher alcohols, acetate esters and ethyl esters being the main aromatic compounds contributing to floral and fruity aromas. The action of yeast, in particular Saccharomyces cerevisiae, on the must components will build the architecture of the wine flavour and its fermentation bouquet. The objective of the present work w...
Intrastrain genomic and phenotypic variability of the commercial Saccharomyces ...
Franco-Duarte, Ricardo; Bigey, Frederic; Carreto, Laura; Mendes, Inês Isabel Moreira Moutinho Vieira; Dequin, Sylvie; Santos, Manuel A. S.; Pais, CéliaThe maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome varia...
Intrastrain genomic and phenotypic variability of the commercial Saccharomyces ...
Franco-Duarte, Ricardo; Bigey, Frédéric; Carreto, Laura; Mendes, Inês; Dequin, Sylvie; Santos, Manuel A. S.; Pais, Célia; Schuller, DoritThe maintenance of microbial species in different environmental conditions is associated with adaptive microevolutionary changes that are shown here to occur within the descendants of the same strain in comparison with the commercial reference strain. However, scarce information is available regarding changes that occur among strain descendants during their persistence in nature. Herein we evaluate genome varia...
Computational Models for Prediction of Yeast Strain Potential for Winemaking fr...
Mendes, Inês Isabel Moreira Moutinho Vieira; Franco-Duarte, Ricardo; Umek, Lan; Fonseca, Elza; Drumonde-Neves, João; Dequin, Sylvie; Zupan, BlazSaccharomyces cerevisiae strains from diverse natural habitats harbour a vast amount of phenotypic diversity, driven by interactions between yeast and the respective environment. In grape juice fermentations, strains are exposed to a wide array of biotic and abiotic stressors, which may lead to strain selection and generate naturally arising strain diversity. Certain phenotypes are of particular interest for th...
Intra-strain phenotypic and genomic variability of the commercial Saccharomyces...
Duarte, Ricardo Franco; Carreto, Laura; Mendes, Inês; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, MargaridaThe use of commercial Saccharomyces cerevisiae wine strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest in large quantities. We showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes, not found among a control group of isolates that derived from clonal expansion of ...
The phenotypic landscape of a Saccharomyces cerevisiae strain collection
Mendes, Inês; Duarte, Ricardo Franco; Umek, Lan; Fonseca, Elza; Neves, J. Drumonde; Dequin, Sylvie; Zupan, Blaz; Schuller, Dorit ElisabethWithin our previous work [1] we developed computational models to predict strains with specific phenotypes (e.g. low ethanol resistance, growth at 30ºC and growth in media containing galactose, raffinose or urea) from microsatellite allelic patterns. The objective of the present work was to gain deeper understanding of the phenotypic diversity of a heterogeneous Saccharomyces cerevisiae strain collection, using...
Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...
Duarte, Ricardo Franco; Carrelo, Laura; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, Dorit Elisabeth; Cambon, BrigitteThe use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced genetic changes that were not found among a control group o...
Genetic characterization of commercial Saccharomyces cerevisiae isolates recove...
Duarte, Ricardo Franco; Carreto, Laura; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, Dorit ElisabethThe use of commercial S. cerevisiae wine strains as fermentation starters has been extensively generalized over the past two decades. Within our previous work we showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes in comparison to the commercial “mother” strain. The objective of the present study was to evaluate genome variations among four isolates o...
Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...
Duarte, Ricardo Franco; Carreto, Laura; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, Dorit ElisabethThe use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced genetic changes that were not found among a control group o...