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Cianobactérias e cianotoxinas em água tratada: conhecer para prevenir

Dias, Elsa; Sarioglou, Konstantina; Ortiz, Miriam; Menezes, Carina

A monitorização da qualidade da água para consumo humano relativamente à presença de cianobactérias é uma das atribuições do Departamento de Saúde Ambiental do Instituto Nacional de Saúde. A proliferação massiva de cianobactérias (bloom) em reservatórios de água doce superficial tem impactos no ecossistema aquático, nos procedimentos operativos das estações de tratamento de água e acarreta riscos para a saúde h...


Metabolomic and taxonomic characterization of Haloleptolyngbya lusitanica sp. n...

Cordeiro, Rita; Luz, Rúben; Lage, Sandra; Menezes, Carina; Dias, Elsa; Flores, Cintia; Fonseca, Amélia; Gonçalves, Vítor

The morphological plasticity of cyanobacteria and their widespread ecological dominance in a wide range of habitats highlights the need for in-depth taxonomic studies. This work focused on the taxonomical revision of Leptolyngbya (Cyanophyceae) strains deposited in the ESSACC culture collection and their metabolomic characterization. Although the studied ESSACC strains were morphologically identified as Leptoly...


Snapshot of resistome, virulome and mobilome in aquaculture

Salgueiro, Vanessa; Manageiro, Vera; Rosado, Tânia; Bandarra, Narcisa M.; Botelho, Maria João; Dias, Elsa; Caniça, Manuela

Aquaculture environments can be hotspots for resistance genes through the surrounding environment. Our objective was to study the resistome, virulome and mobilome of Gram-negative bacteria isolated in seabream and bivalve molluscs, using a WGS approach. Sixty-six Gram-negative strains (Aeromonadaceae, Enterobacteriaceae, Hafniaceae, Morganellaceae, Pseudomonadaceae, Shewanellaceae, Vibrionaceae, and Yersiniacea...


Genomic Analysis of a mcr-9.1-Harbouring IncHI2-ST1 Plasmid from Enterobacter l...

Manageiro, Vera; Salgueiro, Vanessa; Rosado, Tânia; Bandarra, Narcisa M.; Ferreira, Eugénia; Smith, Terry; Dias, Elsa; Caniça, Manuela

This study analyzed the resistome, virulome and mobilome of an MCR-9-producing Enterobacter sp. identified in a muscle sample of seabream (Sparus aurata), collected in a land tank from multitrophic fish farming production. Average Nucleotide Identity analysis identified INSAq77 at the species level as an Enterobacter ludwigii INSAq77 strain that was resistant to chloramphenicol, florfenicol and fosfomycin and w...


Novos valores-guia da Organização Mundial de Saúde para cianotoxinas em águas p...

Dias, Elsa; Rosado, Tânia; Menezes, Carina

As cianobactérias são organismos procariotas fotoautotróficos, presentes numa variedade de habitats, designadamente nas águas doces superficiais. Sendo produtores primários, são essenciais nos ecossistemas, uma vez que estão na base da cadeia trófica e são uma das principais fontes de oxigénio. No entanto, produzem compostos tóxicos (cianotoxinas) que constituem um risco para a saúde pública, sendo a principal ...


Diversity of bacterial communities and genes encoding AMR in different environm...

Manageiro, Vera; Cano, Manuela; Dias, Elsa; Rosado, Tânia; Vieira, Patrícia; Reis, Lígia; Matias, Rui; Rodrigues, João; Coelho, Rodrigo; Menezes, Carina

Aim: The aim of this study was to determine the microbial biodiversity and naturally occurring antibiotic resistance (AR)-encoding genes background load along the food/feed chain in an open-air agricultural testing catchment (HOAL). Methods: Samples were collected in two time points during the year 2020 from various – but interconnected – environmental compartments of the food/feed chain within an HOAL catchmen...


Intoxicação animal por cianobactérias: um alerta para a saúde pública?

Martins, Rita; Pinto, Carlos; Dias, Elsa; Rosado, Tânia; Menezes, Carina

Os perigos associados aos blooms de cianobactérias são conhecidos e percecionados, mas algumas lacunas analíticas e no conhecimento científico impedem, ainda, a devida avaliação do risco de exposição humana a toxinas de cianobactérias e, consequentemente, a adoção das medidas preventivas e de remediação mais adequadas. As cianotoxinas também induzem efeitos nocivos e mortandade em peixes, aves e mamíferos, pelo...


Isolation and characterization of cylindrospermopsis raciborskii strains from f...

Menezes, Carina; Valério, Elisabete; Botelho, Maria João; Dias, Elsa

In the summer of 2015, an intense cyanobacterial bloom producing geosmin/2- methylisoborneol (MIB) occurred in the Roxo freshwater reservoir in Alentejo, Portugal. The drinking water supplied from the Roxo water treatment plant (WTP) exhibited an unpleasant odor/taste and a significant cyanobacteria density was detected in the finished water at the exit of the WTP. Cyanobacteria were not evaluated downstream of...


Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biote...

Guerreiro, Adriana; Andrade, Mariana; Menezes, Carina; Vilarinho, Fernanda; Dias, Elsa

Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those ...


Successful isolation and cultivation of Cylindrospermopsis raciborskii strains ...

Menezes, Carina; Valério, Elisabete; Botelho, Maria João; Martins, Olga; Dias, Elsa

This work presents the successful establishment of Cylindrospermosis raciborskii cultures isolated from water samples collected at the exit point of Water Treatment Plant (WTP). An intense bloom dominated by filamentous cyanobacteria (Aphanizomenon spp., Planktothrix spp., Cylindrospermosis raciborskii, Anabaena spp.) occurred in the summer of 2015 in Roxo reservoir (south Portugal). Several cyanotoxins (microc...


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