2 documents found, page 1 of 1
Identification, characterization, and antifungal activity of cysteine peptidase...
Freitas, Cleverson D. T.; Silva, Rafaela O; Ramos, Márcio Viana; Porfírio, Camila T. M. N.; Farias, Davi F.; Sousa, Jeanlex S; Oliveira, João P. B.Cysteine peptidases (EC 3.4.22) are the most abundant enzymes in latex fluids. However, their physiological functions are still poorly understood, mainly related to defense against phytopathogens. The present study reports the cDNA cloning and sequencing of five undescribed cysteine peptidases from Calotropis procera (Aiton) Dryand (Apocynaceae) as well as some in silico analyses. Of these, three cysteine pepti...
A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activi...
Dias, Lucas P.; Oliveira, Jose T. A.; Rocha-Bezerra, Lady C. B.; Sousa, Daniele O. B.; Costa, Helen P. S.; Araújo, Nadine M. S.; Carvalho, Ana F. U.A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484 Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid sequence is si...