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Provenance study of the limestone used in the construction and restoration of t...

Ding, Y; Mirão, J; Redol, P; Dias, L; Moita, P; Angelini, E; Grassini, S; Schiavon, N

To assess the provenance of the limestones used in the construction and restoration of the Batalha Monastery in central Portugal, stone samples collected from the monument and from five limestone quarries in the region surrounding the building were investigated by energy-dispersive X-ray fluorescence spectroscopy (ED-XRF), powder X-ray diffractometry (PXRD) and thermogravimetric analysis (TGA). Ca-Sr binary dia...


The inhibition of biodegradation on building limestone by plasma etching

Schiavon, Nicola; Caldeira, A.T.; Grassini, S; Ding, Y; Salvador, Cátia

Plasma etching is an innovative technique that has been recently applied in the cleaning of soiled archaeological objects. This research investigated the use of low-pressure plasma etching in cleaning microbial contaminations on an oolitic limestone from an UNESCO World Heritage listed monument: the Batalha Monastery in Central Portugal. The cleaning effect was assessed by FTIR, SEM, optical microscope, and cel...


Biodegradation and Microbial Contamination of Limestone Surfaces: An Experiment...

Ding, Y; Salvador, Cátia; Caldeira, A Teresa; Angelini, E; Schiavon, N

An experimental study was conducted to assess the nature and extent of the biodeterioration of the limestone in the Batalha Monastery in Portugal. Stone fragments covered with microbial biofilms and lichenous crusts were investigated using Optical Microscopy (OM), Low Vacuum Scanning Electron Microscopy with Energy Dispersive Spectroscopy (LV-SEM + EDS), and X-ray micro-Diffractometry ( -XRD). Microbial samples...


Hierarchical structured and programmed vehicles deliver drugs locally to inflam...

Li, W; Li, Y; Liu, Z; Kerdsakundee, N; Zhang, M; Zhang, F; Liu, X; Bauleth-Ramos, T; Lian, W; Mäkilä, E; Kemell, M; Ding, Y; Sarmento, B

Orally administrable drug delivery vehicles are developed to manage incurable inflammatory bowel disease (IBD), however, their therapeutic outcomes are compromised by the side effects of systemic drug exposure. Herein, we use hyaluronic acid functionalized porous silicon nanoparticle to bridge enzyme-responsive hydrogel and pH-responsive polymer, generating a hierarchical structured (nano-in-nano-in-micro) vehi...


Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator

Lince-Faria, M; Maffini, S; Orr, B; Ding, Y; Florindo, C; Sunkel, CE; Tavares, Á; Johansen, J; Johansen, KM; Maiato, H

A putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived...


Chromator is required for proper microtubule spindle formation and mitosis in D...

Ding, Y; Yao, C; Lince-Faria, M; Rath, U; Cai, W; Maiato, H; Girton, J; Johansen, KM; Johansen, J

The chromodomain protein, Chromator, has been shown to have multiple functions that include regulation of chromatin structure as well as coordination of muscle remodeling during metamorphosis depending on the developmental context. In this study we show that mitotic neuroblasts from brain squash preparations from larvae heteroallelic for the two Chromator lossof- function alleles Chro71 and Chro612 have severe ...


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