8 documents found, page 1 of 1
Identificação molecular de amostras de Candida dubliniensis isoladas de lesões ...
Chavasco, Jorge Kleber; Paula, Claudete Rodrigues; Hirata, Mario Hiroyuki; Aleva, Natanael Atilas; Melo, Carlos Eduardo de; Gambale, WalderezCandida dubliniensis é uma nova espécie recentemente descrita. Este patógeno oral emergente compartilha muitas características fenotípicas e bioquímicas com C. albicans dificultando assim a diferenciação entre elas. As mesmas, porém, mostram-se genotipicamente distintas. Este trabalho tem como objetivo identificar, pela técnica de PCR (Polymerase Chain Reaction), a possível presença de C. dubliniensis dentre am...
Cryptococcus neoformans strains isolated from church towers in Rio de Janeiro C...
Baroni, Francisco de Assis; Paula, Claudete Rodrigues; Silva, Ériques Gonçalves da; Viani, Flávio Cesar; Rivera, Irma N.G.Cryptococcosis has been a significant cause of morbidity and mortality in patients with Aids. Many reservoirs of the agent Cryptococcus neoformans have been reported, but the ecology of this yeast must be elucidated in order to establish surveillance programs and to prevent infections. The objective of this study was to evaluate the presence of C. neoformans in Rio de Janeiro City, RJ, Brazil. Ten churches were...
Effectiveness of house dust mite acaricide tri-n-butyl tin maleate on carpets, ...
Uehara, Satiko; Franzolin, Marcia Regina; Chiesa, Soledad; Moreira, Débora; Gambale, Walderez; Paula, Claudete RodriguesThe aim of this study was to determine the effectiveness of the acaricide tri-n-butyl tin maleate, industrially applied to samples of carpets, mattress foam, and fabrics used for furniture upholstery, soft toys and shoe uppers. Approximately 100 adult house dust mites of the species Dermatophagoides pteronyssinus were inoculated into a Petri dish containing the sample (a piece of carpet, mattress foam, or fabri...
Histopathologic and mycologic aspects of experimental infection of guinea pigs ...
Cavalcanti, Josemara Neves; Guerra, José Luiz; Gambale, Walderez; Corrêa, Benedito; Paula, Claudete RodriguesDermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammato...
Isolation and identification of fungai microbiota and of dermatophytes irom hea...
Ishikawa, Marcia Mayumi; Lucas, Ronaldo; Larsson, Carlos Eduardo; Gambale, Walderez; Fernandes, Wilson RobertoDue to the scarcity of reports, in Lafin-American and Brazilian literature, related to fungal microbiota of healthy horses, or to the main species of dermatophytes in horses showing suggestive lesions of dermatophytic infections, 175 horses of both sexes and different ages, including purebred horses, crossbreed, or others with no specified breed were divided in 2 groups. Group 1 was constituted by 133 asymptoma...
Candidin: comparison of two antigens for cutaneous delayed hypersensitivity tes...
Fava-Netto, Celeste; Gambale, Walderez; Croce, Júlio; Paula, Claudete R.; Fava, Sérgio de C.A candidin, which is a suspension of killed yeast cells, is commonly used for intradermal tests of delayed hypersensitivity, to evaluate the immunological cellular competence of the patient, when the test is applied along with other similar tests. When working with a cellular antigen, the histopathology of positive skin tests reveals a cellular infiltrate which not only presents a characteristic hypersensitivit...
Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
Dubugras, Maria Theresa Bonilha; Larsson, Carlos Eduardo; Ledon, Ana Luiza Basso Penteado; Gambale, WalderezFor the diagnosis of superficial mycoses, the clinical aspects and cultivation of fungi for further identification are usually considered. Identification, however, is time consuming and at least 4 weeks are needed for a positive laboratory report. In Brazil, the use of Wood’s light is not a routine screen test, especially in Veterinary Medicine. Thus, the present paper has the aim to evaluate the availability, ...
Fluorescein method (fluorescein diacetate and ethidium bromide) to study the vi...
Corrêa, Benedito; Purchio, Adhemar; Paula, Claudete R.; Gambale, Walderez; Shikanai-Yasuda, Maria AparecidaThe utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspe...