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A first-in-class inhibitor of homologous recombination DNA repair counteracts t...

Calheiros, Juliana; Silva, Rita; Barbosa, Filipa; Morais, João; Moura, Sara Reis; Almeida, Sofia; Fiorini, Elena; Mulhovo, Silva; Aguiar, Tatiana Quinta

The online version contains supplementary material available at: https://doi.org/10.1038/s41467-022-32178-3; Background Pancreatic ductal adenocarcinoma (PDAC) is among the cancer types with poorest prognosis and survival rates primarily due to resistance to standard-of-care therapies, including gemcitabine (GEM) and olaparib. Particularly, wild-type (wt)BRCA tumours, the most prevalent in PDAC, are more resist...


Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture

Aguiar, Tatiana Q.; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, Lucília

Established chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...


Whole yeast cell synthesis of 5-hydroxymethylfurfural-derivatives from apple wa...

Martins, Cristiana; Costa, Carlos E.; Cunha, Joana Filipa Torres Pinheiro; Gullón, Beatriz; Romaní, Aloia; Domingues, Lucília

The transition to renewable resources and bioprocesses is essential to address climate change and meet energy demands. Biorefineries offer a sustainable solution by valorising agro-industrial wastes, such as apple pomace, a by-product of the juice and cider industries. This study presents an efficient process to produce 5-hydroxymethylfurfural (HMF) derivatives using deep eutectic solvent (DES) and microwave-as...


Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae...

Cunha, Joana Filipa Torres Pinheiro; Romaní, Aloia; Domingues, Lucília

Background There is an urgent need to develop bioprocesses independent of fossil resources to address resource depletion and mitigate environmental harm. Transitioning to a bio-based economy requires prioritizing chemical production processes that utilize renewable resources, ensuring sustainability and environmental responsibility. 5-Hydroxymethylfurfural (HMF) and its derivatives are promising building blocks...


Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture

Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, Lucília

Available online 30 October 2024; Established chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site f...


Metabolic versatility of acetogens in syngas fermentation: responding to varyin...

Moreira, João Paulo Carvalho; Domingues, Lucília; Alves, Joana I.

Syngas fermentation using acetogenic bacteria offers a promising route for sustainable chemical production. However, gasliquid mass transfer limitations and efficient co-utilization of CO and H2 pose significant challenges. This study investigated the kinetics of syngas conversion to acetate by Acetobacterium wieringae and Clostridium species in batch conditions under varying initial CO partial pressures (19 11...


Engineering thermotolerant Kluyveromyces marxianus strains for resveratrol prod...

Baptista, Marlene; Costa, Carlos E.; Domingues, Lucília

The microbial biosynthesis of the antioxidant compound resveratrol offers an eco-friendly and sustainable alternative to chemical synthesis or plant extraction. Here, we showed that Kluyveromyces marxianus strains produce p-coumaric acid, a key precursor of resveratrol, with higher titres achieved under increased agitation conditions. Through further strain engineering, resveratrol production was achieved using...


CRISPR‑Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to comba...

Sáez Moreno, David; Cunha, Joana; Melo, Luís Daniel Rodrigues; Tanaka, Kenya; Bamba, Takahiro; Hasunuma, Tomosiha; Azeredo, Joana; Domingues, Lucília

Listeriosis is an infection caused by the consumption of food contaminated with Listeria monocytogenes. It leads to febrile gastroenteritis, central nervous system infections, and even death in risk populations. Bacteriophage endolysins selectively kill bacteria hydrolyzing their cell walls and have emerged as a potential tool for listeriosis control. Ply511 is an anti-Listeria endolysin that has activity again...


Establishing sustainable bioproduction of lactones from metabolic engineering o...

Francisco, Miguel; Freitas, José Luís Silva; Moura, Clara D.; Costa, Carlos E.; Aguiar, Tatiana Quinta; Domingues, Lucília

Introduction: Lactones are volatile organic compounds derived from lipid metabolism. They have a wide range of applications, notably as fragrances and flavours, with -decalactone being particularly valuable. Lactones significantly contribute to the smell and taste of fruits like strawberries and peaches, but traditional extraction methods have many disadvantages. Microbial production has emerged as a more susta...


Enhanced bacterial cellulose production in komagataeibacter sucrofermentans: im...

Silva, Pedro Moreira Montenegro Baptista; Ellis, Tom; Dourado, Fernando; Gama, F. M.; Domingues, Lucília

Background Bacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential. Despite many acetic acid bacteria (AAB) being BC overproducers, cost-effective production remains a challenge. The role of pyrroloquinoline quinone (PQQ)-dependent membrane dehydrogenases (mDH) is crucial in the metabolism of AAB since it links substrate incomplete ...


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