459 documents found, page 1 of 46
Protein jetting-based printing: principles and applications
Sampaio, Ana Regina Simões; Domingues, Lucília; Aguiar, Tatiana QuintaProtein jetting-based printing emerged as a powerful technology at the intersection of biotechnology, materials science, and microfabrication. Building on the intrinsic biocompatibility, biodegradability, and versatility of proteins, it facilitates the development of novel functional materials, by enabling precise, contactless deposition of protein-based inks onto a wide range of substrates while preserving the...
Evaluation of the delivery of an anti-Listeria endolysin via CRISPR-Cas9 engine...
Sáez, David; Carvalho, João Paulo; Murray, Ellen; Colombo, Natalia Soledad Rios; Lamas, Alexandre; Cobas, Alejandra Cardelle; Hill, ColinListeriosis is a foodborne infection caused by Listeria monocytogenes that causes febrile gastroenteritis and central nervous system infections and that can often lead to fatality. Upon consumption of contaminated food, Listeria is able to survive a number of gastrointestinal stressors, including competition with the host microbiota. The emergence of antibiotic-resistant clones of L. monocytogenes, together wit...
Biosynthetic platform for orsellinic acid-derived meroterpenoids in Escherichia...
Tomita, Itsuki; Bamba, Takahiro; Yoshida, Takanobu; Domingues, Lucília; Nasuno, Ryo; Hidese, Ryota; Hasunuma, TomohisaOrsellinic acid (OSA)-derived meroterpenoids, that have an OSA backbone, are plant-derived natural products that have attracted considerable attention as pharmaceutical precursors because of their diverse pharmacological activities. Therefore, developing efficient microbial production methods is highly desirable. However, to date, only a few reports on the microbial production of OSA-derived meroterpenoids are ...
Metabolic versatility of acetogens in syngas fermentation: responding to varyin...
Moreira, João Paulo Carvalho; Domingues, Lucília; Alves, Joana I.Syngas fermentation using acetogenic bacteria offers a promising route for sustainable chemical production. However, gasliquid mass transfer limitations and efficient co-utilization of CO and H2 pose significant challenges. This study investigated the kinetics of syngas conversion to acetate by Acetobacterium wieringae and Clostridium species in batch conditions under varying initial CO partial pressures (19 11...
Assessing process variable effects on laccase-mediated treatment for enhanced s...
Michelin, Michele; Gomes, Daniel Gonçalves; Freitas, Emanuelle N.; Alnoch, Robson C.; Salgado, José Carlos S.; Coelho, Eduardo João LouroLignocellulosic materials (LCM) have gained popularity as a substitute for petroleum, enabling the production of biofuels and various compounds. Eucalyptus bark residue (EBR), generated by pulp and paper mills, is a prime illustration of such materials. The conversion of holocellulose in these materials typically involves the application of enzymes. Nevertheless, these materials may still contain lignin, widely...
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaEstablished chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...
Novel recombinant Human Serum Albumin-SPE systems for ochratoxin A determinatio...
Rodrigues, Diana; Aguiar, Tatiana Quinta; Domingues, Lucília; Abrunhosa, LuísOchratoxin A (OTA) is a mycotoxin that raises food safety concerns in several products, requiring analytical control. Aiming to develop an alternative to immunoaffinity columns (IACs) for OTA extraction and clean-up, recombinant human serum albumin (rHSA) was explored as an affinity bioreceptor for solid-phase extraction (SPE) applications. rHSA was obtained from Pichia pastoris fused with a C-terminal His6 tag...
Engineering thermotolerant Kluyveromyces marxianus strains for resveratrol prod...
Baptista, Marlene; Costa, Carlos E.; Domingues, LucíliaThe microbial biosynthesis of the antioxidant compound resveratrol offers an eco-friendly and sustainable alternative to chemical synthesis or plant extraction. Here, we showed that Kluyveromyces marxianus strains produce p-coumaric acid, a key precursor of resveratrol, with higher titres achieved under increased agitation conditions. Through further strain engineering, resveratrol production was achieved using...
CRISPR‑Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to comba...
Sáez Moreno, David; Cunha, Joana; Melo, Luís Daniel Rodrigues; Tanaka, Kenya; Bamba, Takahiro; Hasunuma, Tomosiha; Azeredo, Joana; Domingues, LucíliaListeriosis is an infection caused by the consumption of food contaminated with Listeria monocytogenes. It leads to febrile gastroenteritis, central nervous system infections, and even death in risk populations. Bacteriophage endolysins selectively kill bacteria hydrolyzing their cell walls and have emerged as a potential tool for listeriosis control. Ply511 is an anti-Listeria endolysin that has activity again...
Harnessing glycerol for secondary metabolite biosynthesis in microorganisms
Kamasaka, Kouhei; Marcello, Luiz; Domingues, Lucília; Hasunuma, TomohisaThe increase in glycerol availability as a byproduct of biodiesel production has attracted attention in its utilization as a carbon source for microbial fermentation. Compared with traditional carbon sources such as glucose, glycerol enhances the production of various secondary metabolites such as aromatic compounds, polyols, and lipids in microorganisms such as Komagataella phaffii and Yarrowia lipolytica. For...