29 documents found, page 1 of 3
A Perspective on the Link between Mitochondria-Associated Membranes (MAMs) and ...
Fernandes, Tânia; Domingues, M. Rosário M.; Moreira, Paula I.; Pereira, Cláudia F.Mitochondria interact with the endoplasmic reticulum (ER) through contacts called mitochondria-associated membranes (MAMs), which control several processes, such as the ER stress response, mitochondrial and ER dynamics, inflammation, apoptosis, and autophagy. MAMs represent an important platform for transport of non-vesicular phospholipids and cholesterol. Therefore, this region is highly enriched in proteins i...
Current trends in the traceability of geographic origin and detection of specie...
Santos, Andreia; Ricardo, Fernando; Domingues, M. Rosário M.; Patinha, Carla; Calado, RicardoMarine bivalves are increasingly consumed worldwide, with their complex supply chain being particularly prone to fraud. This scenario drives economic losses and is a threat to public health, with multiple recent food worries driving consumers to demand more transparency and information on the seafood they buy. To increase consumers confidence in bivalves and enforce current legislation, robust tools are needed ...
Human Bone Marrow Mesenchymal Stromal/Stem Cells Regulate the Proinflammatory R...
Laranjeira, Paula; Pedrosa, Mónia; Duarte, Cátia; Pedreiro, Susana; Antunes, Brígida; Ribeiro, Tânia; Santos, Francisco dos; Martinho, AntónioRheumatoid arthritis (RA) is a disabling autoimmune disease whose treatment is ineffective for one-third of patients. Thus, the immunomodulatory potential of mesenchymal stromal/stem cells (MSCs) makes MSC-based therapy a promising approach to RA. This study aimed to explore the immunomodulatory action of human bone marrow (BM)-MSCs on myeloid dendritic cells (mDCs) and monocytes, especially on cytokines/chemok...
Structural and Functional Alterations in Mitochondria-Associated Membranes (MAM...
Fernandes, Tânia; Resende, Rosa Maria Branco de Matos Costa; Silva, Diana F; Marques, Ana P.; Santos, Armanda Emanuela Castro e; Cardoso, Sandra MoraisAlzheimer's disease (AD) is characterized by the accumulation of extracellular plaques composed by amyloid-β (Aβ) and intracellular neurofibrillary tangles of hyperphosphorylated tau. AD-related neurodegenerative mechanisms involve early changes of mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) and impairment of cellular events modulated by these subcellular domains. In this study, we chara...
Cardiac phospholipidome is altered during ischemia and reperfusion in an ex viv...
Montero-Bullon, Javier-Fernando; Aveiro, Susana S.; Melo, Tânia; Martins-Marques, Tânia; Lopes, Diana; Neves, Bruna; Girão, HenriqueAcute myocardial infarction (AMI) is the leading cause of death, morbidity, and health costs worldwide. In AMI, a sudden blockage of blood flow causes myocardial ischemia and cell death. Reperfusion after ischemia has paradoxical effects and may exacerbate the myocardial injury, a process known as ischemic reperfusion injury. In this work we evaluated the lipidome of isolated rat hearts, maintained in controlle...
Insights on Ultrafiltration-Based Separation for the Purification and Quantific...
Marques, Sara S.; Ramos, Inês I.; Fernandes, Sara; Barreiros, Luisa; Lima, Sofia A. C.; Reis, Salette; Domingues, M. Rosário M.; Segundo, Marcela A.The evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructur...
Halophyte plants from sustainable marine aquaponics are a valuable source of om...
Maciel, Elisabete; Domingues, Pedro; Domingues, M. Rosário M.; Calado, Ricardo; Lillebø, AnaMarine aquaponics is a promising sustainable approach for the production of profitable crops such as halophytes. However, the effect of this culture approach on the lipid composition of halophytes remains unknown. In this work, we contrasted the polar lipidome of Salicornia ramosissima and Halimione portulacoides when produced in marine aquaponics (effluent from a super-intensive flatfish aquaculture production...
Revealing the illegal harvesting of Manila clams (ruditapes philippinarum) usin...
Mamede, Renato; Ricardo, Fernando; Santos, Andreia; Díaz, Seila; Santos, Sónia A.O.; Bispo, Regina; Domingues, M. Rosário M.; Calado, RicardoThe Manila clam (Ruditapes philippinarum) is one of the most traded bivalves in the world. Knowing its harvesting location is therefore paramount to guarantee the safety of consumers. The present study employs fatty acid (FA) profiles of the adductor muscle (AM) to reveal the most likely harvesting location of four batches of Manila clams suspected of having been illegally sourced from the Tagus estuary. In thi...
Oxidized phosphatidylserine mitigates LPS-triggered macrophage inflammatory sta...
Maciel, Elisabete; Neves, Bruno M.; Martins, João; Colombo, Simone; Cruz, Maria Teresa; Domingues, Pedro; Domingues, M. Rosário M.Recent evidence suggests that phosphatidylserine (PS) and its oxidized species drive the clearance of apoptotic cells by macrophages with putative immune response modulation. However, it is not clear whether PS and oxidized PS differentially modulate at molecular level the functional responses of macrophages. Therefore, we proposed in this work to explore this question by evaluating the influence of PS oxidatio...
Lipidomic signature of Bacillus licheniformis I89 during the different growth p...
Lopes, Celestina; Barbosa, Joana; Maciel, Elisabete; da Costa, Elisabete; Alves, Eliana; Domingues, Pedro; Mendo, Sónia; Domingues, M. Rosário M.Bacillus licheniformis I89 is a non-pathogenic, Gram-positive bacterium, frequently found in soil. It has several biotechnological applications as producer of valuable compounds such as proteases, amylases, surfactants, and lantibiotics. Herein, it is reported the identification of the polar lipidome of B. licheniformis I89 during the different growth phases (lag, exponential and stationary) at 37 °C. The analy...