4 documents found, page 1 of 1
Plasma membrane proteomes of differentially matured dendritic cells identified ...
Bernard, Stéphanie Ferret; Borges, William de Castro; Dowle, Adam A.; Sanin, David E.; Cook, Peter C.; Turner, Joseph D.; MacDonald, Andrew S.Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear whatmolecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC–MS/MS to quantify changes in the proteomes. Analysis was performed on triplicate biological samples and changes ...
Abundance of tegument surface proteins in the human blood fluke Schistosoma man...
Borges, William de Castro; Simpson, Deborah M.; Dowle, Adam A.; Curwen, Rachel S.; Oates, Jane Thomas; Beynon, Robert J.; Wilson, R. AlanThe schistosome tegument provides a major interface with the host blood stream in which it resides. Our recent proteomic studies have identified a range of proteins present in the complex tegument structure, and two models of protective immunity have implicated surface proteins as mediating antigens. We have used the QconCAT technique to evaluate the relative and absolute amounts of tegument proteins identified...
Exploring the Fasciola hepatica tegument proteome.
Wilson, R. Alan; Wright, Janelle M.; Borges, William de Castro; Manuel, Sophie J. Parker; Dowle, Adam A.; Ashton, Peter D.; Young, Neil D.The surface tegument of the liver fluke Fasciola hepatica is a syncytial cytoplasmic layer bounded externally by a plasma membrane and covered by a glycocalyx, which constitutes the interface between the parasite and its ruminant host. The tegument’s interaction with the immune system during the fluke’s protracted migration from the gut lumen through the peritoneal cavity and liver parenchyma to the lumen of th...
Enzymatic shaving of the tegument surface of live schistosomes for proteomic an...
Borges, William de Castro; Dowle, Adam A.; Curwen, Rachel S.; Oates, Jane Thomas; Wilson, R. AlanBackground: The membrane-associated and membrane-spanning constituents of the Schistosoma mansoni tegument surface, the parasite’s principal interface with the host bloodstream, have recently been characterized using proteomic techniques. Biotinylation of live worms using membrane-impermeant probes revealed that only a small subset of the proteins was accessible to the reagents. Their position within the multil...