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Bacteriocin dynamics in Enterococcus faecium and Enterococcus lactis: implicati...

Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of con...

A challenge in antibiotic stewardship: detection of vancomycin-variable Enteroc...

Background: Vancomycin-variable-enterococci (VVE) are vanA+ enterococci expressing a vancomycin-susceptible phenotype that can revert to a resistant phenotype (VRE) after vancomycin exposure. Objective: We aimed to screen and characterize VVE in a large collection of Enterococcus faecium (Efm) [1]. Methods: We performed a vanA-PCR screening on an extensive Efm collection (2009-2022), including hospital (n=255) ...

Assessment of antimicrobial resistance in Gram-negative bacteria from rivers su...

Enterococcus spp. from chicken meat collected 20 years apart overcome multiple ...

Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity of selective pressures associated with their maintenance in the poultry-production chain remains poorly explored. We evaluated the susceptibility of Enterococcus spp. from chicken meat collected 20 years apart to antibiotics, metals, acidic pH and peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, ...

Exploring peracetic acid and acidic pH tolerance of antibiotic-resistant non-ty...

Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals’ stomach) or organic acids (e.g., feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains of specific serotypes, clonal lineages, or sources in human/animal pathogens: namely, non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This...

Assessment of antimicrobial resistance spread in surface aquatic systems: a stu...

Antimicrobial resistance (AMR) spread in surface water systems, providing water for drinking or for recreational activities, can have serious implications to human, animal and environmental health. The standard criteria to assess water quality are based in the absence of specific bacteria, and do not include AMR study. Here we evaluate the spread of AMR bacteria in rivers of North Portugal under diverse anthrop...

Uncovering the effects of copper feed supplementation on the selection of coppe...

The use of antibiotics in animal production is linked to the emergence and spread of antibiotic-resistant bacteria, a threat to animal, environmental and human health. Copper (Cu) is an essential element in poultry diets and an alternative to antibiotics, supplementing inorganic or organic trace mineral feeds (ITMF/OTMF). However, its contribution to select multidrug-resistant (MDR) and Cu tolerant Enterococcus...

Enterococcus spp. from chicken meat collected 20 years apart overcome multiple ...

Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity of selective pressures associated with their maintenance in the poultry-production chain remains poorly explored. We evaluated the susceptibility of Enterococcus spp. from chicken meat collected 20 years apart to antibiotics, metals, acidic pH and peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, ...

Effects of a physical exercise program on quality of life and physical fitness ...

To assess the effects of a group class physical exercise program on health-related quality of life (HRQOL), physical fitness and activity, and safety in early breast cancer women after treatment, a double phase trial [16-week control phase (CP) followed by a 16-week intervention phase (IP)] was designed. Outcomes were evaluated at baseline (T1), 8 (T2) and 16 (T3) weeks (CP), and 24 (T4) and 32 (T5) weeks (IP)....

Assessment of antimicrobial resistance spread in surface aquatic systems: a stu...