10 documents found, page 1 of 1
Highly pathogenic Aeromonas hydrophila in swine
Duarte, EL; Queiroga, Maria Cristina; Saavedra, MJOBJECTIVE Autochthonous Iberian pig breeds have been growingly popular due to the increasing demand for locally and extensively produced animals. Due to their different production system, microbiological hazards significantly diverge from industrially reared animals. Within the frame of a broader study to characterize specific pathogens associated with Alentejano pig breed, Aeromonas hydrophila was isolated in ...
Progression of rabbit haemorrhagic disease virus 2 upon vaccination in an indus...
Carvalho, CL; Duarte, EL; Monteiro, J; Afonso; Pacheco; Carvalho; Mendonça; Botelho, A; Albuquerque, T; Themudo, P; Fevereiro, M; Henriques, A.MRabbit haemorrhagic disease virus 2 (RHDV2) emerged recently in several European countries, leading to extensive economic losses in the industry. In response to this new infection, specific inactivated vaccines were developed in Europe and full and rapid setup of protective immunity induced by vaccination was reported. However, data on the efficacy of these vaccines in an ongoing-infection scenario is unavailab...
Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...
Carvalho, CL; Rodeia, J; Branco, Sandra; Monteiro, M; Duarte, EL; Melo, Pedro; Santos, Patricia Tavares; Henriques, Ana Margarida; Santos Barros, SilviaAs key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...
Determinants of the Sympatric Host-Pathogen Relationship in Tuberculosis
David, S; Mateus, AR; Duarte, EL; Albuquerque, J; Portugal, C; Sancho, L; Lavinha, J; Gonçalves G, GMajor contributions from pathogen genome analysis and host genetics have equated the possibility of Mycobacterium tuberculosis co-evolution with its human host leading to more stable sympatric host-pathogen relationships. However, the attribution to either sympatric or allopatric categories depends on the resolution or grain of genotypic characterization. We explored the influence on the sympatric host-pathogen...
Mycobacterium bovis infections in Portugal: Spoligotyping and MIRU-VNTR typing ...
Duarte, EL; Domingos, M; Amado, A; Botelho, ATuberculosis is still one of the most important infectious diseases worldwide. Although human cases due to Mycobacterium bovis, the aetiological agent of bovine tuberculosis (bTB), considerably decreased in most industrialized countries, the possible zoonotic health hazard and the considerable economic losses it brings justify significant efforts to eradicate the disease in several countries. In Portugal, altho...
Molecular epidemiological links between M. Bovis strains isolated from cattle a...
Duarte, EL; Domingos, M; Amado, A; Botelho, ABovine tuberculosis (bTB) prevalence has generally been decreasing for several years in Portugal due to an ongoing UE funded eradication programme. Nevertheless, new outbreaks have been intermittently recorded in bTB free herds, especially in two Eastern regions of Portugal (Beira Interior and Portalegre District part of Alentejo region), with no evident explanation referred in official epidemiological surveys....
RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in G...
Sutre, AF; Sanca, A; Mané, A; Henriques, V; Portugal, C; Sancho, L; Cardoso, A; Paixão, E; Queico, CQF; Salem, JI; Antunes, A; Duarte, EL; David, SThe global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guin...
Genotyping of Portuguese MTC (Mycobacterium tuberculosis complex) animal strains
Duarte, EL; Domingos, M; Amado, A; Botelho, ADuring five years (2002-2007), 295 MTC animal isolates, representing 25% of the total isolations from the bovine TB national eradication programme, were genotyped by spoligotyping and MIRU-VNTR typing. Main purposes were to assess the discriminatory power of different typing techniques and their usefulness for the Portuguese epidemiological scenario, to confirm transmission hypothesis between different cattle h...
Tuberculosis diagnosis after bleach processing for early stage tuberculosis lab...
David, Susana; Sutre, AF; Sanca, A; Mané, A; Henriques, V; Portugal, Clara; Sancho, Luísa; Cardoso, A; Paixão, Eleonora; Duarte, EL; Leite, CQFThe diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement,heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resist...
Implication of the RDRIO Mycobacterium tuberculosis sublineage in multidrug res...
David, Susana; Duarte, EL; Leite, CQF; Paixão, Eleonora; Ribeiro, João Nuno; Maio, José; Portugal, Clara; Sancho, Luísa; Germano de Sousa, JoséMultidrug and extensively drug resistant Mycobacterium tuberculosis are a threat to tuberculosis control programs. Genotyping methods, such as spoligotyping and MIRU–VNTR typing (Mycobacterial Interspersed Repetitive Units), are useful in monitoring potentially epidemic strains and estimating strain phylogenetic lineages and/or genotypic families. M. tuberculosis Latin American Mediterranean (LAM)family is a ma...