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Companion animal owners’ expectations and knowledge on antimicrobial use in Por...

Correia Dias, Margarida; Alpizar Jara, Russel; Lavrador, Catarina; Marques, Catia; Duarte, Elsa Leclerc

While multiple studies have focused on motivations surrounding antimicrobial prescription among companion animal veterinarians, Owners’ expectations and knowledge on antimicrobials and their role on antimicrobial judicious use remains understudied. This work aimed to characterize the knowledge about antimicrobial use and antimicrobial-resistance by pet owners from Portugal. A nationwide survey directed to pet o...


Dermatophytosis in Companion Animals in Portugal: A Comprehensive Epidemiologic...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Angela; Cardoso, Luís; Duarte, Elsa Leclerc; Coelho, Ana Cláudia

Dermatophytosis, commonly referred to as ringworm, is a common superficial fungal infection in companion animals and humans. Between 2012 and 2023, plucked hair and scraped scale samples from domestic dogs and cats with clinical suspicion of dermatophytosis were collected from 355 veterinary medical centres across mainland Portugal. A total of 4716 animal samples were inoculated onto DERM agar, incubated at 25 ...


Feline Infectious Peritonitis Effusion Index: A Novel Diagnostic Method and Val...

Lopes, Ricardo; Sampaio, Filipe; Lima de Carvalho, Hugo; Garcês, Andreia; Fernandes, Cátia; Neves, Carolina Vitória; Sardinha de Brito, Alexandre

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led the medical and scientific community to explore the pathogenesis and clinical manifestations of coronaviruses. In felines, a widespread coronavirus known as feline coronavirus (FCoV) can lead to feline infectious peritonitis (FIP), a highly fatal disease characterised by severe systemic inflammation. Diagnosing FCoV remains ch...


Genetic variants in the IFNGR2 locus associated with severe chronic Q fever

David, Suzana; Castro, Liliana; Duarte, Elsa Leclerc; Gaspar, Ulisses; da Costa Rodrigues, Maria Rosário; Cueto-Rojo, Maria Vanessa; Mendonça, Joana

Q fever is a highly contagious zoonosis capable of causing large outbreaks of important health and economic consequences. Host genetic factors are believed to influence the development of severe chronic Q fever following the infection by the etiological agent, Coxiella burnetii. Targetted next generation sequencing (NGS) was performed in a case-control genetic association study on 53 confirmed Q fever cases, in...


Mapping high-risk areas for Mycobacterium tuberculosis complex bacteria transmi...

Ferreira, Eduardo M; Cunha, Monica; Duarte, Elsa Leclerc; Mira, António; Pinto, Daniela; Mendes, Inês; Pereira, André C; Pinto, Tiago; Acevedo, Pelayo

In many Mediterranean ecosystems, animal tuberculosis (TB), caused by Mycobacterium bovis, an ecovar of Mycobacterium tuberculosis complex (MTBC), is maintained by multi-host communities. It is hypothesised that interspecies transmission is mainly indirect via shared contaminated environments. Therefore, identifying spatial areas where MTBC bacteria occur and quantifying space use by susceptible hosts might hel...


First description of antifungal susceptibility of yeast-like fungi in dermal le...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Ângela; Cardoso, Luís; Duarte, Elsa Leclerc; Coelho, Ana Cláudia

Yeast infections, particularly those caused by species of the genera Malassezia and Candida, are important contributors to otitis and dermatitis in dogs. This study investigates the prevalence and antifungal resistance of various yeast-like fungi isolated from canine dermal lesions in Portugal. Between 2018 and 2023, samples from dogs with clinically suspected yeast infections were collected and analysed using ...


Host-, Environment-, or Human-Related Effects Drive Interspecies Interactions i...

Ferreira, Eduardo M; Cunha, Monica; Duarte, Elsa Leclerc; Gonçalves, Renata; Pinto, Tiago; Mira, António; Santos, Sara M

In many Mediterranean ecosystems, animal tuberculosis (TB), caused by Mycobacterium bovis, is maintained by multi-host communities in which cattle and different wildlife species establish interaction networks contributing to M. bovis transmission and persistence. Most studies have addressed wildlife–cattle disease-relevant interactions, focusing on reservoir hosts, while disregarding the potential contribution ...


Distribution of and Relationships between Epidemiological and Clinicopathologic...

Lopes, Ricardo; Garcês, Andreia; Silva, Augusto; Brilhante-Simões, Paula; Martins, Ângela; Duarte, Elsa Leclerc; Coelho, Ana Cláudia; Cardoso, Luís

Leishmaniosis is a vector-borne disease caused by protozoan parasites of the genus Leishmania, which are zoonotic and have an important impact on animal and public health globally. Between 2009 and 2023, blood samples from domestic dogs with clinical suspicion of leishmaniosis were received from 286 veterinary medical centres throughout mainland Portugal. An enzyme-linked immunosorbent assay (ELISA) was utilise...


Mapping high-risk areas for Mycobacterium tuberculosis complex bacteria transmi...

Ferreira, Eduardo; Cunha, Mónica; Duarte, Elsa Leclerc

In many Mediterranean ecosystems, animal tuberculosis (TB), caused by Mycobacterium bovis, an ecovar of Mycobacterium tuberculosis complex (MTBC), is maintained by multi-host communities. It is hypothesised that interspecies transmission is mainly indirect via shared contaminated environments. Therefore, identifying spatial areas where MTBC bacteria occur and quantifying space use by susceptible hosts might hel...


Disentangling wildlife–cattle interactions in multi-host tuberculosis scenarios...

Ferreira, Eduardo; Duarte, Elsa Leclerc; Cunha, Mónica V; Mira, António; Santos, Sara M

Ecological interactions involving wildlife (wild mammals) and cattle Bos taurus are considered fundamental drivers of animal tuberculosis (TB) caused by Mycobacterium bovis at the wildlife–livestock interface. Despite recent insights about the role of direct and indirect interactions on TB dynamics, a mechanistic evaluation of studies addressing patterns of wildlife–cattle interaction at the global level is lac...


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