50 documents found, page 1 of 5
Tumor‐on‐a‐chip model incorporating human‐based hydrogels for easy assessment o...
Monteiro, Cátia F.; Deus, Inês A.; Silva, Inês B.; Duarte, Iola F.; Custódio, Catarina A.; Mano, João F.The coordinated migration of invasive tumor cells is a complex and dynamic mechanism driven by diverse cellular and molecular events. Unfortunately, the inherent heterogeneity within tumors raises multiple challenges in deciphering key biomarkers and novel therapeutic approaches to prevent tumor metastasis. Here, a microengineered tumor-on-a-chip system incorporating human platelet lysate hydrogels is proposed ...
PM2.5 chemical composition and health risks by inhalation near a chemical complex
Alves, Célia; Evtyugina, Margarita; Estela, Estela; Vicente, Ana; Casotti Rienda, Ismael; Sánchez de la Campa, Ana; Tomé, Mário; Duarte, Iola F.Particulate matter (PM2.5) samples were collected in the vicinity of an industrial chemical pole and analysed for organic and elemental carbon (OC and EC), 47 trace elements and around 150 organic constituents. On average, OC and EC accounted for 25.2% and 11.4% of the PM2.5 mass, respectively. Organic compounds comprised polycyclic aromatic hydrocarbons (PAHs), alkylated PAHs, anhydrosugars, phenolics, aromati...
Metabolic reprogramming of breast tumor-educated macrophages revealed by NMR me...
Dias, Ana S.; Almeida, Catarina R.; Helguero, Luisa A.; Duarte, Iola F.The metabolic crosstalk between tumor cells and tumor-associated macrophages (TAMs) has emerged as a critical contributor to tumor development and progression. In breast cancer (BC), the abundance of immune-suppressive TAMs positively correlates with poor prognosis. However, little is known about how TAMs reprogram their metabolism in the BC microenvironment. In this work, we have assessed the metabolic and phe...
Metabolomics insights into the differential response of breast cancer cells to ...
Garcia-Guasch, Maite; Escrich, Eduard; Moral, Raquel; Duarte, Iola F.The aim of this study was to investigate the effects of two phenolic compounds found in extra virgin olive oil, hydroxytyrosol (HT) and luteolin (LUT), on the metabolism of breast cancer (BC) cells of different molecular subtypes. An untargeted metabolomics approach was used to characterize the metabolic responses of both triple-negative MDA-MB-231 cells and hormone-responsive MCF-7 cells to treatment with thes...
Biological impact of organic extracts from urban-air particulate matter: an in ...
Silva, Tatiana D.; Alves, Célia; Oliveira, Helena; Duarte, Iola F.Atmospheric particulate matter (PM) with diameters below 10 µm (PM10) may enter the lungs through inhalation and are linked to various negative health consequences. Emergent evidence emphasizes the significance of cell metabolism as a sensitive target of PM exposure. However, the current understanding of the relationship between PM composition, conventional toxicity measures, and the rewiring of intracellular m...
Exercise training impacts skeletal muscle remodelling induced by metabolic synd...
Nogueira-Ferreira, Rita; Santos, Inês; Ferreira, Rita; Fontoura, Dulce; Sousa-Mendes, Cláudia; Falcão-Pires, Inês; Lourenço, André P.Metabolic syndrome (MetS), characterized by a set of conditions that include obesity, hypertension, and dyslipidemia, is associated with increased cardiovascular risk. Exercise training (EX) has been reported to improve MetS management, although the underlying metabolic adaptations that drive its benefits remain poorly understood. This work aims to characterize the molecular changes induced by EX in skeletal mu...
Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogen...
Bispo, Daniela S. C.; Michálková, Lenka; Correia, Marlene; Jesus, Catarina S. H.; Duarte, Iola F.; Goodfellow, Brian J.; Oliveira, Mariana B.This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosph...
Metabolic dysregulations underlying the pulmonary toxicity of atmospheric fine ...
Silva, Tatiana D.; Alves, Célia; Oliveira, Helena; Duarte, Iola F.Exposure to atmospheric fne particulate matter (PM2.5) is currently recognized as a leading cause and/or a serious aggravating factor of several respiratory diseases, such as chronic obstructive pulmonary disease (COPD), pulmonary fbrosis, asthma, and lung cancer. Notably, pulmonary-altered metabolism has emerged as an important hallmark of those diseases, while a growing body of evidence shows that exposure to...
Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the ...
da Silva, Gustavo H Rodrigues; Mendes, Luís F.; de Carvalho, Fabíola V.; de Paula, Eneida; Duarte, Iola F.Articaine (ATC) and lidocaine (LDC) are the local anesthetics (LAs) currently most employed in dentistry. Cases of paresthesia, reported more frequently for ATC, have raised concerns about their potential neurotoxicity, calling for further investigation of their biological effects in neuronal cells. In this work, the impact of ATC and LDC on the metabolism of SH-SY5Y cells was investigated through 1H NMR metabo...
Macrophage-targeted shikonin-loaded nanogels for modulation of inflammasome act...
Cardoso, Matias; Gaspar, Vítor M.; Ferreira, Carolina; Silvestre, Ricardo; Duarte, Iola F.; Mano, João F.This study reports the formulation and delivery of hyaluronic acid-Zein (HA-Zein) nanogels loaded with Shikonin (SK) to selectively attenuate macrophage inflammasome. The self-assembled nanogels, produced by nanoprecipitation, exhibited high encapsulation efficiency, and were selectively internalized by human THP-1-derived macrophages without eliciting cytotoxic responses. Cell treatment with HA-Zein-SK nanogel...