5 documents found, page 1 of 1
Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira,...
Carvalho, Carina Luísa; Silva, Sara; Gouveia, Paz; Costa, Margarida; Duarte, Elsa Leclerc; Henriques, Ana Margarida; Barros, Sílvia Santos; Luís, TiagoEmergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira, Portugal (2016–2017)
Challenges in the rabbit haemorrhagic disease 2 (RHDV2) molecular diagnosis of ...
Carvalho, C.L.; Duarte, E.L.; Monteiro, M.; Botelho, A; Albuquerque, T.; Fevereiro, M.; Henriques, A.M.; Barros, S.; Duarte, Margarida DiasMolecular methods are fundamental tools for the diagnosis of viral infections. While interpretation of results is straightforward for unvaccinated animals, where positivity represents ongoing or past infections, the presence of vaccine virus in the tissues of recently vaccinated animals may mislead diagnosis. In this study, we investigated the interference of RHDV2 vaccination in the results of a RT-qPCR for RH...
Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...
Carvalho, CL; Rodeia, Joana; Branco, Sandra; Monteiro, Madalena; Duarte, Elsa Leclerc; Melo, Pedro; Tavares Santos, Patricia; Henriques, Ana MargaridaAs key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...
A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...
Duarte, Margarida Dias; Carvalho, Carina L.; Barros, Silvia C.; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa L.As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...
A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...
Duarte, Margarida Dias; Carvalho, Carina Luísa; Barros, Sílvia; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa LeclercA specific real time RT-PCR for the detection of RHDV2 was developed and validated using RHDV and RHDV2 RNA preparations from positive field samples. The system was designed to amplify a 127 nucleotide-long RNA region located within the vp60 gene, based on the alignment of six sequences originated in Portugal, obtained in our laboratory, and 11 sequences from France and Italy. The primers and probe target seque...