9 documents found, page 1 of 1
Relatório de Estágio: Cine Clube de Viseu
Duarte, Mariana CostaO presente relatório tem como objetivo principal relatar todas as tarefas, experiências e conhecimentos adquiridos durante os cinco meses de estágio no Cine Clube de Viseu. Este resulta da escolha de modalidade de Estágio Curricular para conclusão do Mestrado em Cinema da Universidade da Beira Interior para obtenção de grau de Mestre em Cinema. O relatório encontra-se organizado por capítulos e subcapítulos. In...
New antigens for the serological diagnosis of human visceral leishmaniasis iden...
Carvalho, Ana Maria Ravena Severino; Mendes, Tiago Antônio de Oliveira; Coelho, Eduardo Antonio Ferraz; Duarte, Mariana Costa; Menezes- Souza, DanielVisceral leishmaniasis (VL) still represents a serious public health problem in Brazil due to the inefficiency of the control measures currently employed, that included early diagnosis and treatment of human cases, vector control, euthanasia of infected dogs and, recently approved in Brazil, treatment with Milteforam drug. Effective clinical management depend largely on early and unequivocal diagnosis, however,...
In vivo antileishmanial efficacy of a naphthoquinone derivate incorporated into...
Mendonça, Débora Vasconcelos Costa; Tavares, Grasiele de Sousa Vieira; Lage, Daniela Pagliara; Soyer, Tauane Gonçalves; Carvalho, Lívia MendesNew therapeutic strategies against leishmaniasis are desirable, since the treatment against disease presents problems, such as the toxicity, high cost and/or parasite resistance. As consequence, new antileishmanial compounds are necessary to be identified, as presenting high activity against Leishmania parasites, but low toxicity in mammalian hosts. Flau-A is a naphthoquinone derivative recently showed to prese...
Immunogenomic screening approach to identify new antigens for the serological d...
Elisei, Rutyanne Maria Tonelli; Matos, Christiane Santos; Carvalho, Ana Maria Ravena Severino; Chaves, Ana Thereza; Medeiros, Fernanda Alvarenga CardosoSerological tests are preferentially used for the diagnosis of Chagas’ disease (CD) during the chronic phase because of the low parasitemia and high anti-Trypanosoma cruzi antibody titers. However, the current methods showed several disadvantages, as contradictory or inconclusive results, mainly related to the characteristics of the antigens used, in general, crude or whole parasites, but also due to antigen pr...
Strychnos pseudoquina and its purified compounds present an effective in vitro ...
Lage, Paula Sousa; Andrade, Pedro Henrique Rocha de; Lopes, Amanda de Santana; Fumagalli, Miguel Angel Chávez; Valadares, Diogo GarciaThe development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivi...
Synthesis, antileishmanial activity and QSAR studies of 2-chloro- N -arylacetam...
Lavorato, Stefânia Neiva; Duarte, Mariana Costa; Andrade, Pedro Henrique Rocha De; Coelho, Eduardo Antonio Ferraz; Alves, Ricardo JoséWe describe herein the synthesis and evaluation of the antileishmanial activity against promastigote forms of Leishmania amazonensis and cytotoxicity to murine macrophages of a series of 2-chloro-N-arylacetamide derivatives. All compounds were active, except one (compound 3). Compound 5 presented the most promising results, showing good antileishmanial activity (CI50=5.39±0.67 µM) and moderate selectivity (SI=6...
Poloxamer 407 (Pluronic® F127)-based polymeric micelles for amphotericin B : in...
Mendonça, Débora Vasconcelos Costa; Lage, Letícia Martins dos Reis; Lage, Daniela Pagliara; Chávez Fumagalli, Miguel Angel; Ludolf, FernandaIn the present study, a Poloxamer 407-based amphotericin B (AmpB)-containing polymeric micelles system (AmpB/M) was employed in the treatment of Leishmania amazonensis-infected BALB/c mice. Initially, the in vitro antileishmanial activity (IC50 value) of AmpB/M and B-AmpB/M (empty micelles) against stationary promastigotes and amastigotes-like forms of the parasites was determined, and results were of 1.83 ± 0....
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs u...
Chávez Fumagalli, Miguel Angel; Martins, Vívian Tamietti; Testasicca, Miriam Conceição de Souza; Lage, Daniela Pagliara; Costa, Lourena EmanueleIn Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the pot...
Treatment of murine visceral leishmaniasis using an 8-hydroxyquinoline-containi...
Duarte, Mariana Costa; Lage, Letícia Martins dos Reis; Lage, Daniela Pagliara; Martins, Vivian Tamietti; Carvalho, Ana Maria Ravena SeverinoNewtherapeutics are urgently needed to treat visceral leishmaniasis (VL). Due to the fact that drug discovery is a long and expensive process, the development of delivery systems to carry old and toxic drugs could be considered, as well as the evaluation of new molecules that have already shown to present biological activity. In this context, the present study evaluated the in vitro and in vivo antileishmanial ...