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Glyphosate: a terrestrial threat to marine plants? a study on the seagrass zost...

Deguette, Alizé; Pes, Katia; Vasconcellos, Bernard; Costa, Monya; Silva, João; Barrote, Isabel

Glyphosate-based herbicides (GBHs) are extensively used worldwide, raising concerns about their potential effect on non-target aquatic ecosystems. This study investigated the short-term physiological effects of a commercially available GBH on the seagrass Zostera marina under controlled mesocosm conditions. Z. marina individuals were exposed to three concentrations of glyphosate (0.165, 51, and 5100 mg L−1 ) fo...


Effects of a severe recovery pruning in the productivity and fruit quality of t...

Matias, Pedro; Duarte, Beatriz; Mendonça, Angélica; Oliveira, Silvino; Barrote, Isabel; Guerrero, Carlos; Duarte, Amilcar

Currently, citrus production plays a major role in worldwide agriculture, especially in the Mediterranean. However, the typical irregular branch development and the formation of large canopies and dense exterior foliage can be significant limitations to light entry and air circulation through the canopy. Consequently, dead branches appear in the inner canopy, which becomes unproductive; flowering and vegetative...


Poda de Citrinos na Região Mediterrânica. Manual Técnico

Barrote, Isabel; Neto, Luís; Guerrero, Carlos; Marques, Natália; Duarte, Amilcar

A poda é uma prática comum em citrinos, mas que é por vezes questionada, por se tratar de uma operação dispendiosa e por as árvores não podadas terem produções razoáveis. No entanto, a poda tem provado ser de particular importância na citricultura mediterrânica, orientada para a produção de frutos para consumo em fresco. Nesta citricultura a poda permite aumentar a qualidade do fruto, a qual é altamente valoriz...


Citrus Pruning in the Mediterranean climate: a review

Matias, Pedro; Barrote, Isabel; Azinheira, Gonçalo; Continella, Alberto; Duarte, Amilcar

Pruning is a common practice in citrus for various reasons. These include controlling and shaping the canopy; improving phytosanitary health, productivity, and fruit quality; and facilitating operations such as harvesting and phytosanitary treatments. Because pruning is an expensive operation, its need is sometimes questioned. However, it has been proven to be particularly important in Mediterranean citricultur...


Physiological and morphological effects of a marine heatwave on the seagrass Cy...

Deguette, Alizé; Barrote, Isabel; Silva, João

Marine heatwaves (MHWs) are increasing in frequency and intensity as part of climate change, yet their impact on seagrass is poorly known. The present work evaluated the physiological and morphological responses of Cymodocea nodosa to a MHW. C. nodosa shoots were transplanted into a mesocosm facility. To simulate a MHW, water temperature was raised from 20 to 28 degrees C, kept 7 days at 28 degrees C, cooled do...


Daily regulation of key metabolic pathways in two seagrasses under natural ligh...

Ruocco, Miriam; Barrote, Isabel; Hofman, Jan Dirk; Pes, Katia; Costa, Monya; Procaccini, Gabriele; Silva, João; Dattolo, Emanuela

The circadian clock is an endogenous time-keeping mechanism that enables organisms to adapt to external environmental cycles. It produces rhythms of plant metabolism and physiology, and interacts with signaling pathways controlling daily and seasonal environmental responses through gene expression regulation. Downstream metabolic outputs, such as photosynthesis and sugar metabolism, besides being affected by th...


Heatwave effects on the photosynthesis and antioxidant activity of the seagrass...

Costa, Monya; Silva, João; Barrote, Isabel; Santos, Rui

Global climate change, specifically the intensification of marine heatwaves, affect seagrasses. In the Ria Formosa, saturating light intensities may aggravate heatwave effects on seagrasses, particularly during low spring tides. However, the photophysiological and antioxidant responses of seagrasses to such extreme events are poorly known. Here, we evaluated the responses of <i>Cymodocea nodosa</i> exposed at 2...


m6A RNA Methylation in marine plants: first insights and relevance for biologic...

Ruocco, Miriam; Ambrosino, Luca; Jahnke, Marlene; Chiusano, Maria; Barrote, Isabel; Procaccini, Gabriele; Silva, João; Dattolo, Emanuela

Circadian regulations are essential for enabling organisms to synchronize physiology with environmental light-dark cycles. Post-transcriptional RNA modifications still represent an understudied level of gene expression regulation in plants, although they could play crucial roles in environmental adaptation. N6-methyl-adenosine (m6A) is the most prevalent mRNA modification, established by "writer" and "eraser" p...


Seasonal photosynthesis, respiration, and calcification of a temperate Maërl be...

SORDO, LAURA; Santos, Rui; Barrote, Isabel; Freitas, Cátia; Silva, João

Rhodolith (maerl) beds are biodiversity hotspots with a worldwide distribution. Maerl is the general term for free-living non-geniculate rhodoliths or coralline red algae. In southern Portugal, maerl beds are mainly composed of Phymatolithon lusitanicum, recently identified as a new species and commonly misidentified as Phymatolithon calcareum. Photosynthesis, respiration, and growth rates of the algae were mea...


Projeto PodaCitrus. Dados preliminares de dois ensaios de poda em laranjeira

Matias, Pedro; Oliveira, Silvino; Mendonça, Angélica; Barrote, Isabel; Duarte, Amilcar

Os citrinos têm tendência para formar copas de grande dimensão, com formação de folhagem densa na periferia, o que limita a intensidade luminosa no interior da copa. Nestas condições, os ramos situados no interior acabam por secar e propiciar o desenvolvimento de fungos, ficando a zona produtiva da árvore limitada à zona exterior da copa. Para prevenir estes problemas é necessário realizar uma poda periódica de...


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