5 documents found, page 1 of 1
Rapid detection of Mycobacterium tuberculosis in children using blood and urine...
Costa-Lima,Juliana Figueirêdo da; Pimentel,Lílian Maria Lapa Montenegro; Santos,Fabiana Cristina Fulco; Salazar,Marcela Pereira; Duarte,Rafael SilvaAbstract INTRODUCTION: Laboratory and clinical features of childhood tuberculosis (TB) are non-specific and establishing an accurate diagnosis remains a challenge. This study evaluated a Single tube nested-PCR (STNPCR) to detect genomic DNA of Mycobacterium tuberculosis complex in blood and urine. METHODS: Biological samples were obtained from children (<15 years old) with clinical suspicion of pulmonary and ex...
Rapid detection and differentiation of mycobacterial species using a multiplex ...
Lima,Andrea Santos; Duarte,Rafael Silva; Montenegro,Lilian Maria Lapa; Schindler,Haiana CharifkerIntroduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential d...
Molecular identification and typing of Mycobacterium massiliense isolated from ...
Monego,Fernanda; Duarte,Rafael Silva; Nakatani,Sueli Massumi; Araújo,Wildo Navegantes; Riediger,Irina Nastassja; Brockelt,Sonia; Souza,VerenaOBJECTIVE: One hundred thirty-one cases of postsurgical infections were reported in Southern Region of Brazil between August 2007 and January 2008. Thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak. METHODS: All 39 isolates were analyzed by Zie...
Mycobacterium massiliense BRA100 strain recovered from postsurgical infections:...
Lorena,Nádia Suely de Oliveira; Pitombo,Marcos Bettini; Côrtes,Patrícia Barbur; Maya,Maria Cristina Araújo; Silva,Marlei Gomes daPURPOSE: To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD). METHODS: Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) ...
Infecção por micobactérias de crescimento rápido após procedimentos videocirúrg...
Lorena,Nádia Suely de Oliveira; Duarte,Rafael Silva; Pitombo,Marcos BettiniBetween August 2006 and February 2007, in the state of Rio de Janeiro, Brazil, a massive outbreak of RGM infections after video laparoscopy was mainly associated to the recently described Mycobacterium massiliense species. All confirmed and probable cases reports described the use of high-level disinfection of medical devices by using 2% glutaraldehyde (2% GA) for 30 min before the surgical procedures. We inves...