9 documents found, page 1 of 1
Homemade 3-carbon electrode system for electrochemical sensing: Application to ...
Carneiro, Mariana C.C.G.; Moreira, Felismina T.C.; Dutra, Rosa A.F.; Fernandes, Rúben; Sales, M. Goreti F.The homemade production of carbon screen-printed electrodes (C-SPEs) with a three‑carbon electrode system is reported, along with its application in electrochemical sensing. It is highlighted herein as main novelty that a simple carbon ink may be employed in the preparation of the 3-electrode system, including the pseudo-reference electrode, thereby avoiding the addition of silver or other suitable metal and si...
Detection of cardiac biomarker proteins using a disposable based on a molecular...
Moreira, Felismina T. C.; Sharma, Sanjiv; Dutra, Rosa A.F.; Noronha, João P. C.; Cass, Anthony E. G.; Sales, M. Goreti F.A low-cost disposable was developed for rapid detection of the protein biomarker myoglobin (Myo) as a model analyte. A screen printed electrode was modified with a molecularly imprinted material grafted on a graphite support and incorporated in a matrix composed of poly(vinyl chloride) and the plasticizer o-nitrophenyloctyl ether. The protein-imprinted material (PIM) was produced by growing a reticulated polyme...
Protein-responsive polymers for point-of-care detection of cardiac biomarker
Moreira, Felismina T. C.; Sharma, Sanjiv; Dutra, Rosa A.F.; Noronha, João P. C.; Cass, Anthony E. G.; Sales, M. Goreti F.This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made responsive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film...
Novel biosensing device for point-of-care applications with plastic antibodies ...
Moreira, Felismina T. C.; Dutra, Rosa A.F.; Noronha, João P. C.; Fernandes, João S.; Sales, M. Goreti F.A gold screen printed electrode (Au-SPE) was modified by merging Molecular Imprinting and Self-Assembly Monolayer techniques for fast screening cardiac biomarkers in point-of-care (POC). For this purpose, Myoglobin (Myo) was selected as target analyte and its plastic antibody imprinted over a glutaraldehyde (Glu)/cysteamine (Cys) layer on the gold-surface. The imprinting effect was produced by growing a reticul...
Smart Plastic Antibody Material (SPAM) tailored on disposable screen printed el...
Moreira, Felismina T. C.; Sharma, Sanjiv; Dutra, Rosa A.F.; Noronha, João P. C.; Cass, Anthony E. G.; Sales, M. Goreti F.This work introduces two major changes to the conventional protocol for designing plastic antibodies: (i) the imprinted sites were created with charged monomers while the surrounding environment was tailored using neutral material; and (ii) the protein was removed from its imprinted site by means of a protease, aiming at preserving the polymeric network of the plastic antibody. To our knowledge, these approache...
Electrochemical biosensor based on biomimetic material for myoglobin detection
Moreira, Felismina T. C.; Dutra, Rosa A.F.; Noronha, João P. C.; Sales, M. Goreti F.A novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVCsingle bondCOOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) an...
Surface Imprinting Approach on Screen Printed Electrodes Coated with Carboxylat...
Moreira, Felismina T. C.; Dutra, Rosa A.F.; Noronha, João P. C.; Sales, M. Goreti F.A novel surface molecularly-imprinted (MI) material to detect myoglobin (Myo) using gold screen printed electrodes (SPE) was developed. The sensitive detection was carry out by introducing a carboxylic polyvinyl chloride (PVC-COOH) layer on gold SPE surface. Myo was attached to the surface of gold SPE/PVC-COOH and the vacant spaces around it were filled by polymerizing acrylamide and N,N-methylenebisacrylamide ...
Myoglobin-biomimetic electroactive materials made by surface molecular imprinti...
Moreira, Felismina T. C.; Dutra, Rosa A.F.; Noronha, João P. C.; Sales, M. Goreti F.Myoglobin (Mb) is among the cardiac biomarkers playing a major role in urgent diagnosis of cardiovascular diseases. Its monitoring in point-of-care is therefore fundamental. Pursuing this goal, a novel biomimetic ionophore for the potentiometric transduction of Mb is presented. It was synthesized by surface molecular imprinting (SMI) with the purpose of developing highly efficient sensor layers for near-stereoc...
Artificial antibodies for troponin T by its imprinting on the surface of multiw...
Moreira, Felismina T. C.; Dutra, Rosa A.F.; Noronha, João P. C.; Cunha, Alexandre L.; Sales, GoretiA novel artificial antibody for troponin T (TnT) was synthesized by molecular imprint (MI) on the surface of multiwalled carbon nanotubes (MWCNT). This was done by attaching TnT to the MWCNT surface, and filling the vacant spaces by polymerizing under mild conditions acrylamide (monomer) in N,N′-methylenebisacrylamide (cross-linker) and ammonium persulphate (initiator). After removing the template, the obtained...