3 documents found, page 1 of 1
Multi-species occupancy modeling reveals methodological and environmental effec...
Peixoto, Sara; Mota-Ferreira, Mário; Chaves, Cátia; Velo-Antón, Guillermo; Beja, Pedro; Egeter, BastianAquatic environmental DNA is increasingly used for biodiversity monitoring, such as surveying threatened and invasive species. Mainstreaming these methods in practi- cal applications, however, still requires significant standardization and optimisation, namely regarding DNA capture methods. Here we evaluated how filter type (standard disc filters vs high-capacity capsules), number of sampling sites, volume of w...
Species detection from aquatic eDNA: assessing the importance of capture methods
Peixoto, Sara; Chaves, Cátia; Velo-Antón, Guillermo; Beja, Pedro; Egeter, BastianEnvironmental DNA (eDNA) is increasingly used for biodiversity monitoring, particularly in aquatic systems. However, each step, from sample collection to bioinformatic analysis, can introduce biases and influence the reliability of results. While much effort has been put into the optimization of laboratory methods, less attention has been devoted to estimate the impacts of eDNA capture methods. To address this ...
Modelling technical and biological biases in macroinvertebrate community assess...
Martins, Filipa M.S.; Porto, Miguel; Feio, Maria J.; Egeter, Bastian; Bonin, Aurélie; Serra, Sónia R.Q.; Taberlet, Pierre; Beja, PedroDNA metabarcoding from the ethanol used to store macroinvertebrate bulk samples is a convenient methodological option in molecular biodiversity assessment and biomonitoring of aquatic ecosystems, as it preserves specimens and reduces problems associated with sample sorting. However, this method may be affected by errors and biases, which need to be thoroughly quantified before it can be mainstreamed into biomon...