7 documents found, page 1 of 1
A regulatory perspective on the applicability of NAMs in genotoxicity and carci...
Bossa, Cecilia; Alivernini, Silvia; Andreoli, Cristina; Aquilina, Gabriele; Attias, Leonello; Benfenati, Emilio; Dusinska, Maria; El Yamani, NaoualeNew Approach Methodologies (NAMs) are gaining significant momentum globally to reduce animal testing and enhance the efficiency and human relevance of chemical safety assessment. Even with substantial EU commitment from regulatory agencies and the academic community, the full regulatory adoption of NAMs remains a distant prospect. This challenge is further complicated by the fact that the academic world, orient...
A regulatory perspective on the applicability of NAMs in genotoxicity and carci...
Bossa, Cecilia; Alivernini, Silvia; Andreoli, Cristina; Aquilina, Gabriele; Attias, Leonello; Benfenati, Emilio; Dusinska, Maria; El Yamani, NaoualeNew Approach Methodologies (NAMs) are gaining significant momentum globally to reduce animal testing and enhance the efficiency and human relevance of chemical safety assessment. Even with substantial EU commitment from regulatory agencies and the academic community, the full regulatory adoption of NAMs remains a distant prospect. This challenge is further complicated by the fact that the academic world, orient...
A critical review to identify data gaps and improve risk assessment of bispheno...
Mhaouty-Kodja, Sakina; Zalko, Daniel; Tait, Sabrina; Testai, Emanuela; Viguié, Catherine; Corsini, Emanuela; Grova, Nathalie; Buratti, Franca MariaBisphenol A (BPA), a synthetic chemical widely used in the production of polycarbonate plastic and epoxy resins, has been associated with a variety of adverse effects in humans including metabolic, immunological, reproductive, and neurodevelopmental effects, raising concern about its health impact. In the EU, it has been classified as toxic to reproduction and as an endocrine disruptor and was thus included in ...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
Contribution to the hazard assessment of benchmark metallic nanomaterials throu...
Vital, Nádia; El Yamani, Naouale; Pinhão, Mariana; Rúden-Pra, Elise; Louro, Henriqueta; Dušinská, Maria; Silva, Maria JoãoMetallic nanomaterials are among the most commonly applied manufactured nanomaterials (NM). Regardless of the considerable efforts to assess their safety, the presently available data do not allow for general and consistent conclusions. Moreover, the validity of the classical in vitro testing systems for NMs hazard assessment has been questioned and needs confirmation. In the present study, the toxicity of thre...
Contribution to the hazard assessment of benchmark metallic nanomaterials throu...
Vital, Nádia; El Yamani, Naouale; Pinhão, Mariana; Rúden-Pra, Elise; Louro, Henriqueta; Dušinská, Maria; Silva, Maria JoãoBackground and Aim: Metall-based nanomaterials (NMs) are among the most commonly applied manufactured nanomaterials (NM). Regardless of the considerable efforts to assess their safety, the presently available data do not allow for general and consistent conclusions. Moreover, the validity of the classical in vitro testing systems for NMs hazard assessment has been questioned and needs confirmation. The present ...
Mutagenic and carcinogenic potential of metal nanoparticles depends on their ph...
Dusinska, Maria; El Yamani, Naouale; Fjellsbø, Lise Maria; Huk, Anna; Gabelova, Alena; Alonso, Tamara Iglesias; Silva, Maria João; Pran, Elise RundenNanomaterials (NMs) and nanoparticles (NPs) have been studied intensively for almost two decades and still all the characteristics that might be beneficial or harmful are not explored. Although many studies have investigated the mechanisms of action of NPs in cells, results are not fully understood and in many cases are controversial. Endpoints appropriate for detecting harmful chemicals such as cytotoxicity, o...