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Health status in gilthead seabream (Sparus aurata) juveniles fed diets devoid o...

Reis, B.; Ramos-Pinto, L.; Martos-Sitcha, J. A.; Machado, M.; Azeredo, R.; Fernandez-Boo, S.; Engrola, S.; Unamunzaga, C.; Calduch-Giner, J.

To enhance fish general health, feeds can be supplemented with health-promoting additives, reducing the need to use chemotherapeutics. Incorporation of marine algae biomasses in aquafeeds has been shown to improve fish immune status by enhancing innate immune response. This study evaluated the effects of Phaeodactylum tricornutum incorporation in feed by two different processes, either as freeze-dried biomass o...


Daily feeding and protein metabolism rhythms in Senegalese sole post-larvae

Navarro, Carmen; Yufera, Manuel; Engrola, S.

Fish hatcheries must adapt larval feeding protocols to feeding behavior and metabolism patterns to obtain more efficient feed utilization. Fish larvae exhibit daily ingesting rhythms rather than ingesting food continuously throughout the day. The aim of this study was to determine the daily patterns of feed intake, protein digestibility, protein retention and catabolism in Senegalese sole post-larvae (Solea sen...


Dietary glucose stimulus at larval stage modifies the carbohydrate metabolic pa...

Rocha, Filipa; Dias, Jorge; Geurden, Inge; Dinis, Maria Teresa; Panserat, Stephane; Engrola, S.

The concept of nutritional programming was investigated in order to enhance the use of dietary carbohydrates in gilthead seabream juveniles. We assessed the long-term effects of high-glucose stimuli, exerted at the larval stage, on the growth performance, nutrient digestibility and metabolic utilization and gene expression of seabream juveniles, challenged with a high-carbohydrate intake. During early developme...


High-glucose feeding of gilthead seabream (Sparus aurata) larvae: effects on mo...

Rocha, Filipa; Dias, Jorge; Geurden, Inge; Dinis, Maria Teresa; Panserat, Stephane; Engrola, S.

Nutritional programming has begun to arouse interest as a novel tool to alter specific metabolic pathways or functions in farmed animals. The aim of the present study was to explore the potential of early glucose stimuli to induce changes in nutrient metabolism of gilthead seabream. Nutritional conditioning was performed by delivering glucose-rich feed at three distinct recurrent periods of larval feeding regim...


The supplementation of a microdiet with crystalline indispensable amino-acids a...

Canada, Paula; Engrola, S.; Mira, Sara; Teodósio, Rita; Fernandes, Jorge M. O.; Sousa, Vera; Barriga-Negra, Lúcia; Conceicao, Luis; Valente, Luisa M. P.

The full expression of growth potential in fish larvae largely depends on an efficient protein utilization, which requires that all the indispensable amino acids (IAAs) are provided at an optimum ratio. The effect of supplementing a practical microdiet with encapsulated crystalline-AA to correct possible IAA deficiencies was evaluated in Senegalese sole larvae. Two isonitrogenous and isoenergetic microdiets wer...


New developments and biological insights into the farming of Solea senegalensis...

Morais, Sofia; Aragão, C.; Cabrita, Elsa; Conceição, L. E. C.; Constenla, Maria; Costas, Benjamín; Dias, Jorge; Duncan, Neil; Engrola, S.

Senegalese sole was one of the earliest identified candidate species with high potential for aquaculture diversification in the south of Europe. Its culture has been possible, and commercially attempted, for several decades, but intensive production has been slow to take off. This has been explained mostly by serious disease problems, high mortality at weaning, variable growth and poor juvenile quality. However...


Can Senegalese sole post-larvae effectively grow on low dietary DHA and lipid l...

Pinto, Wilson; Engrola, S.; Santos, André; Bandarra, Narcisa M.; Dias, Jorge; Conceicao, Luis

Senegalese sole (Solea senegalensis) is a highly-valuable flatfish species with interest for diversification of aquaculture in Mediterranean countries. Unlike other fish species known to have a high dietary DHA requirement during the larval stage, several studies have suggested dietary DHA could play a minor role for Senegalese sole during the early developmental stages. This suggestion was recently supported b...


Dietary indispensable amino acids profile affects protein utilization and growt...

Canada, Paula; Engrola, S.; Richard, Nadège; Lopes, Ana Filipa; Pinto, Wilson; Valente, Luísa M. P.; Da Conceição, Gisele Cristina

In diet formulation for fish, it is critical to assure that all the indispensable amino acids (IAA) are available in the right quantities and ratios. This will allow minimizing dietary AA imbalances that will result in unavoidable AA losses for energy dissipation rather than for protein synthesis and growth. The supplementation with crystalline amino acids (CAA) is a possible solution to correct the dietary ami...


Glucose metabolism and gene expression in juvenile zebrafish (Danio rerio) chal...

Rocha, Filipa; Dias, Jorge; Engrola, S.; Gavaia, Paulo; Geurden, Inge; Dinis, Maria Teresa; Panserat, Stephane

Knowledge on the role of early nutritional stimuli as triggers of metabolic pathways in fish is extremely scarce. The objective of the present study was to assess the long-term effects of glucose injection in the yolk (early stimulus) on carbohydrate metabolism and gene regulation in zebrafish juveniles challenged with a high-carbohydrate low-protein (HC) diet. Eggs were microinjected at 1 d post-fertilisation ...


Glucose overload in yolk has little effect on the long-term modulation of carbo...

Rocha, Filipa; Dias, J.; Engrola, S.; Gavaia, Paulo J.; Geurden, Inge; Dinis, Maria Teresa; Panserat, S.

The use of early nutritional stimuli to program metabolic pathways in fish is ill defined. Therefore, studies were undertaken with zebrafish to assess the effect of high glucose levels during the embryonic stage as a lifelong modulator of genes involved in carbohydrate metabolism. Genes related to carbohydrate metabolism were expressed at low levels at 0.2 and 1 day post-fertilization (dpf). However, from 4 dpf...


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