53 documents found, page 1 of 6
Pericardial fluid accumulates microRNAs that regulate heart fibrosis after myoc...
Silva, Elsa D.; Pereira-Sousa, Daniel; Ribeiro-Costa, Francisco; Cerqueira, Rui; Enguita, Francisco J.; Gomes, Rita N.; Dias-Ferreira, JoãoPericardial fluid (PF) has been suggested as a reservoir of molecular targets that can be modulated for efficient repair after myocardial infarction (MI). Here, we set out to address the content of this biofluid after MI, namely in terms of microRNAs (miRs) that are important modulators of the cardiac pathological response. PF was collected during coronary artery bypass grafting (CABG) from two MI cohorts, pati...
Space radiation damage rescued by inhibition of key spaceflight associated miRNAs
McDonald, J. Tyson; Kim, JangKeun; Farmerie, Lily; Johnson, Meghan L.; Trovao, Nidia S.; Arif, Shehbeel; Siew, Keith; Tsoy, Sergey; Bram, YaronOur previous research revealed a key microRNA signature that is associated with spaceflight that can be used as a biomarker and to develop countermeasure treatments to mitigate the damage caused by space radiation. Here, we expand on this work to determine the biological factors rescued by the countermeasure treatment. We performed RNA-sequencing and transcriptomic analysis on 3D microvessel cell cultures expos...
RNA Regulatory Networks 2.0
Enguita, Francisco J.; Leitão, Ana Lúcia; Mattick, John S.The central role of RNA molecules in cell biology has been an expanding subject of study since the proposal of the "RNA world" hypothesis 60 years ago [...].
Dengue virus capsid protein facilitates genome compaction and packaging
Boon, Priscilla L. S.; Silva Martins, Ana; Lim, Xin Ni; Enguita, Francisco J.; Santos, Nuno C.; Bond, Peter J.; Wan, Yue; Martins, Ivo C.Dengue virus (DENV) is a single-stranded (+)-sense RNA virus that infects humans and mosquitoes, posing a significant health risk in tropical and subtropical regions. Mature virions are composed of an icosahedral shell of envelope (E) and membrane (M) proteins circumscribing a lipid bilayer, which in turn contains a complex of the approximately 11 kb genomic RNA with capsid (C) proteins. Whereas the structure o...
The non-coding RNA journal club: highlights on recent papers-10
Pinzon Cortes, Jairo A.; El-Osta, Assam; Fontemaggi, Giulia; Delihas, Nicholas; Miyazaki, Katsuki; Goel, Ajay; Brazane, Mira; Carré, ClémentWe are delighted to share with you our seventh Journal Club and highlight some of the most interesting papers published recently. We hope to keep you up-to-date with non-coding RNA research works that are outside your study area. The Non-Coding RNA Scientific Board wishes you an exciting and fruitful read.
Cx43‐mediated sorting of miRNAs into extracellular vesicles
Martins Marques, Tânia; Costa, Marina C.; Catarino, Steve; Simões, Isaura; Aasen, Trond; Enguita, Francisco J.; Girao, HenriqueThrough the exchange of lipids, proteins, and nucleic acids, extracellular vesicles (EV) allow for cell-cell communication across distant cells and tissues to regulate a wide range of physiological and pathological processes. Although some molecular mediators have been discovered, the mechanisms underlying the selective sorting of miRNAs into EV remain elusive. Previous studies demonstrated that connexin43 (Cx4...
Association between miR-146a and tumor necrosis factor alpha (TNF-α) in stable ...
Pereira-da-Silva, Tiago; Napoleão, Patricia; Costa, Marina C.; Gabriel, André F.; Selas, Mafalda; Silva, Filipa; Enguita, Francisco J.Background and Objectives: Tumor necrosis factor alpha (TNF-α) is proatherogenic and associated with the risk of acute ischemic events, although the mechanisms that regulate TNF-α expression in stable coronary artery disease (SCAD) are not fully understood. We investigated whether metabolic, inflammatory, and epigenetic (microRNA (miRNA)) markers are associated with TNF-α expression in SCAD. Materials and Metho...
Circulating miRNAs are associated with the systemic extent of atherosclerosis :...
Pereira da Silva, Tiago; Napoleão, Patricia; Costa, Marina C.; Gabriel, André F.; Selas, Mafalda; Silva, Filipa; Enguita, Francisco J.The mechanisms that regulate the systemic extent of atherosclerosis are not fully understood. We investigated whether the expression of circulating miRNAs is associated with the extent of stable atherosclerosis to a single territory or multiple territories (polyvascular) and with the severity of atherosclerosis in each territory. Ninety-four participants were prospectively recruited and divided into five age- a...
Structural insights into carboxylic polyester-degrading enzymes and their funct...
Leitão, Ana Lúcia; Enguita, Francisco J.Esters are organic compounds widely represented in cellular structures and metabolism, originated by the condensation of organic acids and alcohols. Esterification reactions are also used by chemical industries for the production of synthetic plastic polymers. Polyester plastics are an increasing source of environmental pollution due to their intrinsic stability and limited recycling efforts. Bioremediation of ...
Systematic structure-based search for Ochratoxin-degrading enzymes in Proteomes...
Leitão, Ana Lúcia; Enguita, Francisco J.Background: ochratoxins are mycotoxins produced by filamentous fungi with important implications in the food manufacturing industry due to their toxicity. Decontamination by specific ochratoxin-degrading enzymes has become an interesting alternative for the treatment of contaminated food commodities. Methods: using a structure-based approach based on homology modeling, blind molecular docking of substrates and ...