2 documents found, page 1 of 1
Revealing higher than expected meiofaunal diversity in Antarctic sediments: a m...
Fonseca, V. G.; Sinniger, F.; Gaspar, J. M.; Quince, C.; Creer, S.; Power, Deborah M.; Peck, Lloyd S.; Clark, Melody S.An increasing number of studies are showing that Antarctic mega- and macrofauna are highly diverse, however, little is known about meiofaunal biodiversity in sediment communities, which are a vital part of a healthy and functional ecosystem. This is the first study to analyse community DNA (targeting meiofauna) using metabarcoding to investigate biodiversity levels in sediment communities of the Antarctic Penin...
Sample richness and genetic diversity as drivers of chimera formation in nSSU m...
Fonseca, V. G.; Nichols, B.; Lallias, D.; Quince, C.; Carvalho, Gary; Power, Deborah; Creer, S.Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras are hybrid products composed of distinct parental sequences that can lead to the misinterpretation of diversity es...