4 documents found, page 1 of 1
Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organ...
Silva, Teresa P.; Sousa-Luis, Rui; Fernandes, Tiago G.; Bekman, Evguenia; Rodrigues, Carlos A.V.; Vaz, Sandra H.; Moreira, Leonilde M.; Hashimura, YasHuman-induced pluripotent stem cells (iPSCs) have great potential for disease modeling. However, generating iPSC-derived models to study brain diseases remains a challenge. In particular, the ability to recapitulate cerebellar development in vitro is still limited. We presented a reproducible and scalable production of cerebellar organoids by using the novel single-use Vertical-Wheel bioreactors, in which funct...
Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrog...
Fernandes, Cláudia S.M.; Rodrigues, André L.; Delgado Alves, Vitor; Fernandes, Tiago G.; Pina, Ana Sofia; Roque, Ana Cecília A.Tissue engineering and stem cell research greatly benefit from cell encapsulation within hydrogels as it promotes cell expansion and differentiation. Affinity-triggered hydrogels, an appealing solution for mild cell encapsulation, rely on selective interactions between the ligand and target and also on the multivalent presentation of these two components. Although these hydrogels represent a versatile option to...
Extracellular vesicles in CNS developmental disorders
Gomes, Ana Rita; Sangani, Nasim Bahram; Fernandes, Tiago G.; Diogo, Maria Margarida; Curfs, Leopold M. G.; Reutelingsperger, Chris P.The central nervous system (CNS) is the most complex structure in the body, consisting of multiple cell types with distinct morphology and function. Development of the neuronal circuit and its function rely on a continuous crosstalk between neurons and non-neural cells. It has been widely accepted that extracellular vesicles (EVs), mainly exosomes, are effective entities responsible for intercellular CNS commun...
Modeling Rett syndrome with human patient-specific forebrain organoids
Gomes, Ana Rita; Fernandes, Tiago G.; Vaz, Sandra H.; Silva, Teresa P.; Bekman, Evguenia; Xapelli, Sara; Duarte, Sofia; Ghazvini, MehrnazEngineering brain organoids from human induced pluripotent stem cells (hiPSCs) is a powerful tool for modeling brain development and neurological disorders. Rett syndrome (RTT), a rare neurodevelopmental disorder, can greatly benefit from this technology, since it affects multiple neuronal subtypes in forebrain sub-regions. We have established dorsal and ventral forebrain organoids from control and RTT patient-...