5 documents found, page 1 of 1
Remodelling of the cardiac extracellular matrix proteome during chronological a...
Santinha, Deolinda; Vilaça, Andreia; Estronca, Luís; Schüler, Svenja; Bartoli, Catherine; Sandre-Giovannoli, Annachiara De; Figueiredo, Arnaldo
Induced pluripotent stem cell-derived vascular networks to screen nano-bio inte...
Estronca, Luís; Francisco, Vitor; Pitrez, Patrícia; Honório, Inês; Carvalho, Lara; Vazão, Helena; Blersh, Josephine; Rai, Akhilesh; Nissan, XavierThis work was funded by FEDER through the Program COMPETE and by Portuguese fund through FCT in context of the projects PTDC/SAU-TOX/121887/2010, POCI-01-0145-FEDER-029229, POCI-01-0145-FEDER-016390 and POCI-01-0145-FEDER-029414, as well as the European project ERAatUC (ref. 669088). PRP wishes to thank FCT for a BD fellowship (SFRH/BD/71042/2010). The iMM Fish Facility was supported by CONGENTO LISBOA-01-0145-...
Induced pluripotent stem cell-derived vascular networks to screen nano-bio inte...
Estronca, Luís; Francisco, Vitor; Pitrez, Patrícia; Honório, Inês; Carvalho, Lara; Vazão, Helena; Blersch, Josephine; Rai, Akhilesh; Nissan, XavierThe vascular bioactivity/safety of nanomaterials is typically evaluated by animal testing, which is of low throughput and does not account for biological differences between animals and humans such as ageing, metabolism and disease profiles. The development of personalized human in vitro platforms to evaluate the interaction of nanomaterials with the vascular system would be important for both therapeutic and r...
Vulnerability of progeroid smooth muscle cells to biomechanical forces is media...
Pitrez, Patricia R.; Estronca, Luís; Monteiro, Luís Miguel; Colell, Guillem; Vazão, Helena; Santinha, Deolinda; Harhouri, Karim; Thornton, DanielHutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disease in children that leads to early death. Smooth muscle cells (SMCs) are the most affected cells in HGPS individuals, although the reason for such vulnerability remains poorly understood. In this work, we develop a microfluidic chip formed by HGPS-SMCs generated from induced pluripotent stem cells (iPSCs), to study their vulnerability to flow...
Molecular etiology of atherogenesis--in vitro induction of lipidosis in macroph...
Estronca, Luís; Silva, Joao Carlos Pinho; Sampaio, Julio L.; Shevchenko, Andrej; Verkade, Paul; Vaz, Alfin D. N.; Vaz, Winchil L. C.; Vieira, Otília V.Background: Atherosclerosis starts by lipid accumulation in the arterial intima and progresses into a chronic vascular inflammatory disease. A major atherogenic process is the formation of lipid-loaded macrophages in which a breakdown of the endolysomal pathway results in irreversible accumulation of cargo in the late endocytic compartments with a phenotype similar to several forms of lipidosis. Macrophages exp...