6 documents found, page 1 of 1
Rational Design of Cost-Effective 4-Styrylcoumarin Fluorescent Derivatives for ...
Eustáquio, Raquel; Ramalho, João Paulo; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels are key tools in a wide range of modern scientific applications, such as fluorescence microscopy, flow cytometry, histochemistry, direct and indirect immunochemistry, and fluorescence in situ hybridization (FISH). Small fluorescent labels have important practical advantages as they allow maximizing the fluorescence signal by binding multiple fluorophores to a single biomolecule. At present, t...
New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecu...
Eustáquio, Raquel; Caldeira, Ana Teresa; Arantes, Sílvia; Candeias, António; Pereira, AntónioFluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. Thes...
Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules
Eustáquio, Raquel; Ramalho, João Paulo; Arantes, Sílvia; Candeias, António; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as...
New 4-styrylcoumarin derivatives as potentials fluorescent labels for biomolecu...
Eustáquio, Raquel; Caldeira, Ana Teresa; Arantes, Sílvia; Candeias, António; Pereira, AntónioCurrently, fluorescence microscopy is one of the highly sensitive imaging techniques that allow visualize, detect, and track biomolecules in analytical studies in many important scientific areas as cellular biology, environmental sciences, medicine, pharmacy, among others. One of the great advantages of fluorescence microscopy is the possibility to use several fluorescent labels to detect different biomolecules...
Synthesis of new inexpensive fluorescent water-soluble amine-reactive reagents ...
Eustáquio, Raquel; Caldeira, Ana Teresa; Arantes, Sílvia; Candeias, António; Pereira, AntónioCultural heritage assets are being continuously submitted to physical, chemical and biological degradation. Biodegradation is the major factor for their deterioration, with microorganisms being of critical importance due to their metabolism and proliferation. In this context, it is frequently necessary identify and locate individual microorganisms and detect small amounts of biomolecules, as proteins, to know, ...
Inexpensive small molecules as promising fluorescent labels for biomolecules.
Eustáquio, Raquel; Ramalho, João Paulo; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels are indispensable in various modern scientific applications, including direct and indirect immunochemistry, fluorescence microscopy, histochemistry, flow cytometry and fluorescence in situ hybridization (FISH) [1,2]. Small fluorescent labels offer significant practical benefits, enabling the optimization of fluorescence signals through the attachment of multiple fluorophores to a single biomo...