9 documents found, page 1 of 1
Ms-222 and propofol sedation during and after the simulated transport of nile t...
Félix, LM; Correia, R; Sequeira, R; Ribeiro, C; Monteiro, S; Antunes, L; Silva, J; Venâncio, C; Valentim, AThe use of anesthetics has been suggested as a strategy to hamper live fish transport-induced stress. Still, there is insufficient data available on the use of alternative anesthetics to MS-222. This study investigated the use of propofol to mitigate stress in Nile tilapia (Oreochromis niloticus, 143.8 ± 20.9 g and 20.4 ± 0.9 cm) during a 6 h simulated transport. Individuals (n = 7) were divided into three grou...
Teratogenic, oxidative stress and behavioural outcomes of three fungicides of n...
Vieira, R; Venâncio, C; Félix, LMThe improper use of synthetic fungicides has raised public concerns related to environmental pollution and animal health. Over the years, plant-derived antifungals have been investigated as safer alternatives, although little scientific evidence of its neurodevelopmental effects exist. The main objective of this study was to explore the effects of three alternative natural extracts (Equisetum arvense, Mimosa te...
Multi-biomarker responses of Asian clam Corbicula fluminea (bivalvia, corbiculi...
Parra, S; Varandas, S; Santos, D; Félix, LM; Fernandes, L; Cabecinha, E; Gago, J; Monteiro, SMOne of the most widespread aquatic organisms in the rivers and estuarine ecosystems, in the world, is Asian clam Corbicula fluminea. This clam, that can adapt to environmental changes, is an invasive species in several areas and it was adopted as a model for toxicity tests. This study evaluated the effects of the exposure to cadmium (Cd), to microplastics (MPs) and their mixtures on C. fluminea. The oxidative s...
Embryonic zebrafish response to a commercial formulation of azoxystrobin at env...
Vieira, RSF; Venâncio, CAS; Félix, LMAzoxystrobin is a broad-spectrum strobilurin fungicide for use on a wide range of crops available to end-users as formulated products. Due to its extensive application, it has been detected in aquatic ecosystems, raising concerns about its environmental impact, which is still poorly explored. The objective of this work was to study the effects of a commercial formulation of azoxystrobin in the zebrafish embryo ...
Inflammatory, oxidative stress, and apoptosis effects in zebrafish larvae after...
Lanzarin, G; Venâncio, C; Félix, LM; Monteiro, SGlyphosate-based herbicides (GBH) are the most used herbicides in the world, carrying potentially adverse consequences to the environment and non-target species due to their massive and inadequate use. This study aimed to evaluate the effects of acute exposure to a commercial formulation of glyphosate, Roundup® Flex (RF), at environmentally relevant and higher concentrations in zebrafish larvae through the asse...
Dietary supplementation with chestnut (Castanea sativa) reduces abdominal adipo...
Rodrigues, P; Ferreira, T; Nascimento-Gonçalves, E; Seixas, F; Costa, RMG; Martins, T; Neuparth, MJ; Pires, MJ; Lanzarin, G; Félix, LM; Venâncio, CThe production of chestnut (Castanea sativa Miller) is mostly concentrated in Europe. Chestnut is recognized by its high content of antioxidants and phytosterols. This work aimed to evaluate the effects of dietary chestnut consumption over physiological variables of FVB/n mice. Eighteen FVB/n male 7-month-old mice were randomly divided into three experimental groups (n = 6): 1 (control group) fed a standard die...
MS-222 short exposure induces developmental and behavioural alterations in zebr...
Félix, LM; Luzio, A; Themudo, M; Antunes, L; Matos, M; Coimbra, AM; Valentim, AMMS-222 has been widely used as an anaesthetic in fish, thus, raising the need to infer about its toxicological safety during development. In this study, MS-222 toxicity in zebrafish embryos was evaluated after a 20-min exposure at different stages of development. Embryos exposed during the 256-cell stage displayed an increase in mortality, associated with defective early developmental pathways. Following exposu...
Ketamine induction of p53-dependent apoptosis and oxidative stress in zebrafish...
Félix, LM; Vidal, AM; Serafim, C; Valentim, AM; Antunes, LM; Monteiro, SM; Matos, M; Coimbra, AMKetamine is a widely used pharmaceutical that has been detected in water sources worldwide. Zebrafish embryos were used in this study to investigate the oxidative stress and apoptotic signals following a 24h exposure to different ketamine concentrations (0, 50, 70 and 90 mg L-1). Early blastula embryos (∼2 h post fertilisation-hpf) were exposed for 24 h and analysed at 8 and 26 hpf. Reactive oxygen species and ...
Evaluation of anaesthetic protocols for laboratory adult zebrafish (Danio rerio)
Martins, T; Diniz, E; Félix, LM; Antunes, LIn the last decades, the use of zebrafish (Danio rerio) in biomedical research has increased. Anaesthesia is daily used in fish during experimental procedures to avoid discomfort, stress or pain. Also, fish welfare and the reliability of results can be compromised if an unsuitable anaesthetic protocol is used. Therefore, we aimed to refine anaesthetic protocols to be used in adult zebrafish by evaluating the ef...