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Comparative study on cellular and molecular responses in oyster sperm revealed ...

F RIESCO, MARTA; Félix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa

The Portuguese (Crassostrea angulata) and Pacific oyster (Crassostrea gigas), both from the genus Crassostrea, are two important species for production and conservation. Although they have common characteristics, different susceptibilities to rearing conditions have been described in these species. Overall, in the case of C. angulata, only a few remaining populations are present in the south of Portugal and Spa...


First study in cryopreserved Crassostrea angulata sperm

F RIESCO, MARTA; Félix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa

Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the ...


Molecular basis of spermatogenesis and sperm quality

Robles, Vanesa; Herraez, Paz; Labbe, Catherine; Cabrita, Elsa; Psenicka, Martin; Valcarce, David G.; F RIESCO, MARTA

Spermatozoan quality can be evaluated in different ways, here we focus on the analysis of DNA, RNA and epigenetic status of germ cells. These characterizations also can be the bases for explaining sperm quality at other levels, so we will see how some of these molecules could affect other sperm quality markers. Moreover, we consider the possibility of using some of these molecules as predictors of sperm quality...


Biology of teleost primordial germ cells (PGCs) and spermatogonia: Biotechnolog...

Robles, Vanesa; F RIESCO, MARTA; Psenicka, Martin; Saito, Taiju; Valcarce, David G.; Cabrita, Elsa; Herraez, Paz

This review provides a general view on teleost germline development using primordial germ cells (PGCs) or spermatogonia (SG), highlighting recent progress in research on these two cell types in teleost fishes. Due to the interest of these cells for gene banking purposes, this chapter also reviews the available protocols for cryopreservation and the techniques that have been employed to assess cellular and molec...


Solea senegalensis sperm cryopreservation: new insights on sperm quality

F RIESCO, MARTA; Oliveira, Catarina; Soares, Florbela; Gavaia, Paulo J.; Dinis, Maria Teresa; Cabrita, Elsa

Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to iden...


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