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West Nile virus transmission potential in Portugal

Lourenço, José; Barros, Sílvia C.; Zé-Zé, Líbia; Damineli, Daniel S. C.; Giovanetti, Marta; Osório, Hugo; Amaro, Fátima; Henriques, Ana M.

It is unclear whether West Nile virus (WNV) circulates endemically in Portugal. Despite the country's adequate climate for transmission, Portugal has only reported four human WNV infections so far. We performed a review of WNV-related data (1966-2020), explored mosquito (2016-2019) and land type distributions (1992-2019), and used climate data (1981-2019) to estimate WNV transmission suitability in Portugal. Se...


Depression in midlife woman: A study with users of primary health services

Gonçalves, Bruno; Fagulha, Teresa; Ferreira, Ana

This study is based on the data collected in an investigation regarding Primary Health Care patients. The prevalence of depressive disorders is studied in a sample of women with ages ranging between 35 and 65 years. All women (n = 179) responded to the Center for Epidemiologic Studies Depression Scale (CES-D), and 127 agreed to participate in a structured clinical interview adapted from the evaluation of mood d...

Date: 2018   |   Origin: Revista Psicologia

Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira,...

Carvalho, Carina Luísa; Silva, Sara; Gouveia, Paz; Costa, Margarida; Duarte, Elsa Leclerc; Henriques, Ana Margarida; Barros, Sílvia Santos; Luís, Tiago

Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira, Portugal (2016–2017)


Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...

Carvalho, CL; Rodeia, J; Branco, Sandra; Monteiro, M; Duarte, EL; Melo, Pedro; Santos, Patricia Tavares; Henriques, Ana Margarida; Santos Barros, Silvia

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit ...

Carvalho, CL; Rodeia, Joana; Branco, Sandra; Monteiro, Madalena; Duarte, Elsa Leclerc; Melo, Pedro; Tavares Santos, Patricia; Henriques, Ana Margarida

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...

Duarte, Margarida Dias; Carvalho, Carina L.; Barros, Silvia C.; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa L.

As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild rabbit repopulation of depleted areas assigned for the lynx re-introduction. Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located in Bar...


A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus...

Duarte, Margarida Dias; Carvalho, Carina Luísa; Barros, Sílvia; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa Leclerc

A specific real time RT-PCR for the detection of RHDV2 was developed and validated using RHDV and RHDV2 RNA preparations from positive field samples. The system was designed to amplify a 127 nucleotide-long RNA region located within the vp60 gene, based on the alignment of six sequences originated in Portugal, obtained in our laboratory, and 11 sequences from France and Italy. The primers and probe target seque...


Snapshot of viral infections in wild carnivores reveals ubiquity of parvovirus ...

Duarte, Margarida D.; Henriques, Ana Margarida; Barros, Sílvia Carla; Fagulha, Teresa; Mendonça, Paula; Carvalho, Paulo; Monteiro, Madalena

The exposure of wild carnivores to viral pathogens, with emphasis on parvovirus (CPV/FPLV), was assessed based on the molecular screening of tissue samples from 128 hunted or accidentally road-killed animals collected in Portugal from 2008 to 2011, including Egyptian mongoose (Herpestes ichneumon, n = 99), red fox (Vulpes vulpes, n = 19), stone marten (Martes foina, n = 3), common genet (Genetta genetta, n = 3)...


Detection of very virulent infectious bursal disease virus in vaccinated broilers

Fagulha, Teresa; Fevereiro, Miguel

Infectious bursal disease (IBD), usually referred as Gumboro disease, is an acute and highly contagious immunosuppressive disease of young chickens. The etiological agent is infectious bursal disease virus (IBDV), a nonenveloped, icosahedral virus of the Birnaviridae family with a bisegmented, double-stranded RNA genome. The clinical signs are usually observed between 3 and 6 weeks and include whitish or watery...


Genótipo QX-like do vírus da bronquite infecciosa detectado em Portugal

Fagulha, Teresa; Fevereiro, Miguel

O vírus da bronquite infecciosa (IB) pertence ao género Gammacoronavirus da família Coronaviridae e é o agente etiológico da bronquite infecciosa, uma doença aguda e altamente contagiosa dos tractos respiratório superior e urogenital de galinhas de todas as idades, responsável por perdas económicas consideráveis dentro da indústria avícola. Os sintomas incluem desconforto respiratório, estertores traqueais, esp...


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