102 documents found, page 1 of 11
Effect of hyperbaric oxygenation on random rat skin flaps vascularization
Rech,Fabricio Valandro; Simões,Ricardo Santos; Pires,Jefferson André; Florêncio-Silva,Rinaldo; Fagundes,Djalma JoséABSTRACT Purpose: To evaluate the effect of hyperbaric oxygenation (HBO) on angiogenesis in random rat skin flaps, by immunoexpression of vascular endothelial growth factor A (VEGF-A). Methods: Forty adult rats were divided into four groups: GE) epilated; GE/HBO) epilated subjected to HBO; GER) epilated submitted to dorsal skin flap; GER/HBO) epilated subjected to dorsal skin flap + HBO. HBO was performed with ...
The role of ischemic preconditioning in the expression of apoptosis-related gen...
Miranda,Celina Teresa Castelo Branco Couto de; Fagundes,Djalma José; Miranda,Edinaldo de; Simões,Ricardo Santos; Carbonel,Adriana Aparecida FerrazAbstract Purpose: To analyze the effects of ischemic preconditioning (IPC) in the expression of apoptosis-related genes in rat small intestine subjected to ischemia and reperfusion. Methods: Thirty anesthetized rats underwent laparotomy and were drive into five groups: control (CG); ischemia (IG); ischemia and reperfusion (IRG); IPC and ischemia (IG+IPC); IPC and ischemia and reperfusion (I/RG+IPC). Intestinal ...
Effect of hyperbaric oxygenation on the expression of glutathione peroxidase 4 ...
Ikejiri,Adauto Tsutomu; Somaio Neto,Frederico; Bertoletto,Paulo Roberto; Chaves,José Carlos; WakateTeruya,Alexandre Key; Kassuya,Cândida Aparecida LeiteAbstract Purpose: To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR). Methods: Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to ...
The role of the exogenous supply of adenosine triphosphate in the expression of...
Fagundes,Djalma José; Carrara,Fernando Lopes; Teixeira,William Andrade; Simões,Ricardo Santos; Taha,Murched OmarAbstract Purpose: To investigate the role of the exogenous supply of adenosine triphosphate (ATP) in the expression of Bax and Bcl2L1 genes in intestinal ischemia and reperfusion (IR) in rats. Methods: The study was designed as a randomized controlled trial with a blinded assessment of the outcome. Eighteen adult male Wistar-EPM1 rats were housed under controlled temperature and light conditions (22-23°C, 12 h ...
Knockdown of transglutaminase-2 prevents early age-induced vascular changes in ...
Armstrong,Dinani Matoso Filho; Sikka,Gautam; Armstrong,Anderson da Costa; Saad,Karen Ruggeri; Freitas,William Rodrigues de; Berkowitz,Dan EzraAbstract Purpose: To determine whether the absence of transglutaminase 2 enzyme (TG2) in TG2 knockout mice (TG2-/-) protect them against early age-related functional and histological arterial changes. Methods: Pulse wave velocity (PWV) was measured using non-invasive Doppler and mean arterial pressure (MAP) was measured in awake mice using tail-cuff system. Thoracic aortas were excised for evaluation of endothe...
The role of atenolol in the modulation of the expression of genes encoding pro-...
Taha,Murched Omar; Silva,Thaís de Melo Alexandre e; Ota,Keimy Saori; Vilela,Wander Junqueira; Simões,Ricardo Santos; Starzewski Junior,AlbertoAbstract Purpose: To investigate the role of atenolol in the gene expression of caspase 1 (Casp1) and Bcl2L1 on vascular endothelium of rat intestine after ischemia and reperfusion (IR). Methods: Eighteen adult male Wistar rats were randomly divided into 3 groups (n=6): SG (Sham group): no clamping of the superior mesenteric artery; IRG: IR plus saline group: IRG+At: IR plus Atenolol group. Rats from IRG and IR...
The role of ischemic preconditioning in gene expression related to inflammation...
Miranda,Celina Teresa Castelo Branco Couto de; Fagundes,Djalma José; Miranda,Edinaldo de; Simões,Ricardo Santos; Taha,Murched OmarAbstract Purpose: To investigate the gene expression related to inflammation on mice subjected to intestinal ischemia and reperfusion (I/R) and treated with ischemic preconditioning (IPC). Methods: Thirty rats (EPM-Wistar), distributed in five groups of six animals each, were underwent anesthesia and laparotomy. The ischemia time was standardized in 60 minutes and the reperfusion time 120 minutes. IPC was stand...
The role of ischemic preconditioning and pentoxifylline in intestinal ischemia/...
Oliveira,Teresinha Regina Ribeiro de; Oliveira,Geraldo Ferreira de; Simões,Ricardo Santos; Tikazawa,Eduardo Hiroshi; Monteiro,Hugo PequenoAbstract Purpose: To investigate the role of ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal mucosa ischemia/reperfusion injury (IR). Methods: Thirty rats were assigned to 5 groups (N=6): (CG): no clamping of the superior mesenteric artery (90 min.); (IR-SS): saline + ischemia (30 min.) + reperfusion (60 min.); (IR-PTX): PTX + ischemia (30min.) + reperfusion (60 min.); (IPC-IR-SS): 5 min. ...
Hyperbaric oxygenation and the genic expression related to oxidative stress in ...
Somaio Neto,Frederico; Ikejiri,Adauto Tsutomo; Bertoletto,Paulo Roberto; Chaves,José Carlos; Teruya,Roberto; Fagundes,Djalma JoséAbstract Purpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, wh...
The expression of endothelial and inducible nitric oxide synthase and apoptosis...
Oliveira,Teresinha Regina Ribeiro de; Oliveira,Geraldo Ferreira de; Simões,Ricardo Santos; Feitosa,Suellen Maurim; Tikazawa,Eduardo HiroshiAbstract Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX)...