93 documents found, page 1 of 10
Hazard identification and characterization of leachable chemicals from plastic ...
Dirven, Hubert; Bogusz, Aleksandra; Hans Bouwmeester; Busch, Mathias; Duflos, Guillaume; Eriksen, Gunnar S.; Fardilha, Margarida; Flores-Gomez, DanielaA recent study has suggested that plastics may contain more than 16,000 chemicals, including additives, processing aids, starting substances, intermediates and Non-Intentionally Added Substances. Plastic chemicals are released throughout the plastic life cycle, from production, use, disposal and recycling. Most of these chemicals have not been studied for potential hazardous properties for humans and in the env...
Rabbit sperm sexing by targeting membrane proteins specific to X- or Y-chromoso...
Pinho, Patrícia Pinto de; Fardilha, Margarida; Leite, Rosário PintoIn animal production systems, the significance of sex pre-selection cannot be overstated, particularly in industries like cuniculture where the economic value of animals varies greatly based on sex. Despite advancements in reproductive technologies, addressing the surplus of animals of unwanted sex remains an ongoing challenge. Existing technologies such as flow cytometry have been successfully implemented in s...
Emerging roles of the Protein Phosphatase 1 (PP1) in the context of viral infec...
Corda, Pedro O.; Bollen, Mathieu; Ribeiro, Daniela; Fardilha, MargaridaProtein Phosphatase 1 (PP1) is a major serine/threonine phosphatase in eukaryotes, participating in several cellular processes and metabolic pathways. Due to their low substrate specificity, PP1's catalytic subunits do not exist as free entities but instead bind to Regulatory Interactors of Protein Phosphatase One (RIPPO), which regulate PP1's substrate specificity and subcellular localization. Most RIPPOs bind...
The Impact of Lifestyle on Prostate Cancer: A Road to the Discovery of New Biom...
Leitão, Catarina; Matos, Bárbara; Roque, Fátima; Herdeiro, Maria Teresa; Fardilha, MargaridaProstate cancer (PCa) is one of the most common cancers among men, and its incidence has been rising through the years. Several risk factors have been associated with this disease and unhealthy lifestyles and inflammation were appointed as major contributors for PCa development, progression, and severity. Despite the advantages associated with the currently used diagnostic tools [prostate-specific antigen(PSA) ...
A peptide-centric approach to analyse quantitative proteomics data- an applicat...
Lima, Tania; Rodrigues, João Eduardo; Manadas, Bruno; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiBottom-up proteomics is a popular approach in molecular biomarker research. However, protein analysts have realized the limitations of protein-based approaches for identifying and quantifying proteins in complex samples, such as the identification of peptides sequences shared by multiple proteins and the difficulty in identifying modified peptides. Thus, there are many exciting opportunities to improve analysis...
Galectin-3 in prostate cancer and heart diseases: a biomarker for these two fri...
Lima, Tânia; Perpétuo, Luís; Henrique, Rui; Fardilha, Margarida; Leite-Moreira, Adelino; Bastos, Jose; Vitorino, RuiGalectin-3 (Gal-3) belongs to galectin protein family, a type of β-galactose-binding lectin having more than one evolutionarily conserved domain of carbohydrate recognition. Gal-3 is mainly located in the cytoplasm, but it also enters the nucleus and is secreted into the extracellular environment and biological fluids such as urine, saliva, and serum. It plays an important role in many biological functions, suc...
Gal-3 Protein Expression and Localization in Prostate Tumours
Lima, Tânia; Macedo-Silva, Catarina; Felizardo, Diana; Fraga, João; Carneiro, Isa; Jerónimo, Carmen; Henrique, Rui; Fardilha, Margarida; Vitorino, RuiGal-3 plays an important role in cell survival, mRNA splicing, and cell-cell and cell-matrix interactions. Depending on its cellular localization and cancer type, Gal-3 may have tumour-suppressive or tumour-promoting activities. Given the promising diagnostic role of Gal-3 in the urine of PCa patients found in our previous study, its concordant gene and protein expression levels, and its involvement in PCa-rela...
Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify...
Corda, Pedro O.; Moreira, Jéssica; Howl, John; Oliveira, Pedro F.; Fardilha, Margarida; Silva, Joana VieiraThe advent of proteomics provides new opportunities to investigate the molecular mechanisms underlying male infertility. The selection of relevant targets based on a single analysis is not always feasible, due to the growing number of proteomic studies with conflicting results. Thus, this study aimed to systematically review investigations comparing the sperm proteome of normozoospermic and infertile men to def...
Appraising Animal Models of Prostate Cancer for Translational Research: Future ...
Nascimento Gonçalves, Elisabete; Seixas, Fernanda; Da Costa, Rui M. Gil; Pires, Maria Joao; Neuparth, Maria Joao; Moreira-Goncalves, DanielThe growing incidence of prostate cancer has prompted a great investment in basic biology and translational studies to develop new therapies. Multiple animal models have been established to study etiological factors, cancer-preventive strategies and the molecular determinants of aggressiveness and metastases. The rat model of prostate cancer induced by chemical carcinogen N-methyl-N-nitrosourea (MNU) and testos...
Smoking Induces a Decline in Semen Quality and the Activation of Stress Respons...
Henriques, Magda Carvalho; Santiago, Joana; Patrício, António; Herdeiro, Maria Teresa; Loureiro, Susana; Fardilha, MargaridaMale infertility is a prevalent concern affecting couples worldwide. While genetic factors, hormonal imbalances, and reproductive system defects play significant roles, emerging evidence suggests that lifestyle choices also profoundly impact male fertility. This study aimed to explore the effects of several lifestyle factors, including tobacco and alcohol consumption, physical activity, and dietary habits, on s...