6 documents found, page 1 of 1
Molecular investigation of ready-to-eat salads for Giardia duodenalis and Crypt...
Faria, Clarissa Perez; Pereira, Adelaide; Almeida, Daniela; Pinto, Miguel; Lourenço, Ágata; Sousa, Maria do CéuFoodborne outbreaks are often associated with the consumption of salads. However, published studies on the detection of foodborne pathogens in ready-to-eat salads are scarce. The aim of this study was to detect Giardia duodenalis and Cryptosporidium DNA in ready-to-eat salads, by applying techniques of molecular biology to study the frequency of contamination in salads. A total of 100 packages of ready-to-eat s...
Lipidome of extracellular vesicles from Giardia lamblia
Faria, Clarissa Perez; Ferreira, Bárbara; Lourenço, Ágata; Guerra, Inês Calado; Melo, Tânia; Domingues, Pedro; Domingues, Maria do Rosário MarquesExtracellular vesicles (EVs) (exossomes, microvesicles and apoptotic bodies) have been well acknowledged as mediators of intercellular communications in prokaryotes and eukaryotes. Lipids are essential molecular components of EVs but at the moment the knowledge about the lipid composition and the function of lipids in EVs is limited and as for now none lipidomic studies in Giardia EVs was described. Therefore, ...
Serological prevalence of toxoplasmosis in pregnant women in Luanda (Angola): G...
Vueba, Amélia Nkutxi; Faria, Clarissa Perez; Almendra, Ricardo; Santana, Paula; Sousa, Maria do CéuWe report a study on toxoplasmosis in pregnant women in Luanda, Angola, determining the seroprevalence, geospatial distribution and its association with socio-economic features, dietary habits and hygiene and health conditions. Anti-Toxoplasma gondii IgG and IgM were quantified in serum samples of women attended at the Lucrecia Paim Maternity Hospital between May 2016 and August 2017. The IgG avidity test and q...
Giardia lamblia Decreases NF-κB p65RelA Protein Levels and Modulates LPS-Induce...
Faria, Clarissa Perez; Neves, Bruno Miguel; Lourenço, Ágata; Cruz, Maria Teresa; Martins, João D.; Silva, Ana; Pereira, Sónia; Sousa, Maria do CéuThe protozoan Giardia lamblia is the most common cause of parasitic gastrointestinal infection worldwide. The parasite developed sophisticated, yet not completely disclosed, mechanisms to escape immune system and growth in the intestine. To further understand the interaction of G. lamblia with host immune cells, we investigated the ability of parasites to modulate the canonical activation of mouse macrophages (...
Geospatial distribution of intestinal parasitic infections in Rio de Janeiro (B...
Faria, Clarissa Perez; Zanini, Graziela Maria; Dias, Gisele Silva; da Silva, Sidnei; de Freitas, Marcelo Bessa; Almendra, Ricardo; Santana, PaulaBackground Intestinal parasitic infections remain among the most common infectious diseases worldwide. This study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of Rio de Janeiro, considering demographic, socio-economic, and epidemiological contextual factors. Methods/Principal findings The cross-section survey w...
Molecular Characterization of Giardia lamblia: First Report of Assemblage B in ...
Faria, Clarissa Perez; Zanini, Graziela Maria; Dias, Gisele Silva; da Silva, Sidnei; Sousa, Maria do CéuBackground Despite the high prevalence of giardiasis, the genetic characterization of Giardia lamblia has been poorly documented in Brazil and molecular epidemiology research has only been conducted in the last few years. The aim of this study was to determine the prevalence of different G. lamblia assemblages and detect mixed infections among patients with giardiasis. Methods and Principal Findings The cross-s...