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Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production

Fontes, Afonso; Francisco, Ricardo; Ferreira, Frederico Castelo; Faria, Nuno Torres; Marques, Susana; Reis, Alberto; Moura, Patrícia; Lukasik, Rafal M.

ABSTRACT: Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further culti...

Date: 2024   |   Origin: Repositório do LNEG

Integrated perspective on microbe-based production of itaconic acid: from metab...

Nascimento, Miguel Figueiredo; Marques, Nuno; Correia, Joana; Faria, Nuno Torres; Mira, Nuno P.; Ferreira, Frederico Castelo

ABSTRACT: The discovery of itaconic acid as a product of citric acid pyrolytic distillation in 1837 opened the possibility of using it as a polymer building block. Itaconic acid, featuring two carboxylic acids and an unsaturated group, can potentially be used as a building block in several chemical syntheses, with a particular emphasis on polymer manufacture. The elucidation of biochemical pathways originating ...

Date: 2022   |   Origin: Repositório do LNEG

Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: ...

Nascimento, Miguel Figueiredo; Coelho, Tiago; Reis, Alberto; Gouveia, Luisa; Faria, Nuno Torres; Ferreira, Frederico Castelo

ABSTRACT: Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process economy. Herein, we report for the first time MELs production using oils produced from microalgae. The bio-oi...

Date: 2022   |   Origin: Repositório do LNEG

Integrated perspective on microbe-based production of itaconic acid: from metab...

Nascimento, Miguel Figueiredo; Marques, Nuno; Correia, Joana; Faria, Nuno Torres; Mira, Nuno P.; Ferreira, Frederico Castelo

ABSTRACT: The discovery of itaconic acid as a product of citric acid pyrolytic distillation in 1837 opened the possibility of using it as a polymer building block. Itaconic acid, featuring two carboxylic acids and an unsaturated group, can potentially be used as a building block in several chemical syntheses, with a particular emphasis on polymer manufacture. The elucidation of biochemical pathways originating ...

Date: 2022   |   Origin: Repositório do LNEG

Production of Mannosylerythritol Lipids Using Oils from Oleaginous Microalgae: ...

Nascimento, Miguel Figueiredo; Coelho, Tiago; Reis, Alberto; Gouveia, Luisa; Faria, Nuno Torres; Ferreira, Frederico Castelo

ABSTRACT: Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process economy. Herein, we report for the first time MELs production using oils produced from microalgae. The bio-oi...

Date: 2022   |   Origin: Repositório do LNEG

High cellulase-free xylanases production by Moesziomyces aphidis using low-cost...

Faria, Nuno Torres; Marques, Susana; Cerejo, Joana; Vorobieva, Ekaterina; Fonseca, César

ABSTRACT: Background Enzymes involved in xylan hydrolysis have several industrial applications. Selection of efficient microbial hosts and scalable bioreaction operations can lower enzyme production costs and contribute to their commercial deployment. This work aims at investigating the Moesziomyces aphidis yeast cultivation conditions that deliver maximal xylanase titres, yields and productivities using low-co...

Date: 2022   |   Origin: Repositório do LNEG

Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, ...

Nascimento, Miguel Figueiredo; Barreiros, Ricardo; Oliveira, Ana Cristina; Ferreira, Frederico Castelo; Faria, Nuno Torres

ABSTRACT: Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, high production costs, low productivity and unsatisfactory scale-up production have hampered commercial adoption. Herein, we report for the first time the beta-galactosidase production by Moesziomyces spp. from different sugars (D-galactose, D-glucose and D-lac...

Date: 2022   |   Origin: Repositório do LNEG

Production of mannosylerythritol lipids using oils from oleaginous microalgae: ...

Nascimento, Miguel Figueiredo; Coelho, Tiago; Reis, Alberto; Gouveia, Luísa; Faria, Nuno Torres; Ferreira, Frederico Castelo

Mannosylerythritol lipids (MELs) are biosurfactants with excellent biochemical properties and a wide range of potential applications. However, most of the studies focusing on MELs high titre production have been relying in the use of vegetable oils with impact on the sustainability and process economy. Herein, we report for the first time MELs production using oils produced from microalgae. The bio-oil was extr...


Direct xylan conversion into glycolipid biosurfactants,mannosylerythritol lipid...

Faria, Nuno Torres; Marques, Susana; Fonseca, César; Ferreira, Frederico Castelo

Mannosylerythritol lipids (MEL) are glycolipid biosurfactants, produced by Pseudozyma spp., with increasing commercial interest. While MEL can be produced from d-glucose and d-xylose, the direct conversion of the respective lignocellulosic polysaccharides, cellulose and xylan, was not reported yet. The ability of Pseudozyma antarctica PYCC 5048T and Pseudozyma aphidis PYCC 5535T to use cellulose (Avicel®) and x...

Date: 2015   |   Origin: Repositório do LNEG

Production of glycolipid biosurfactants, mannosylerythritol lipids, from pentos...

Faria, Nuno Torres; Santos, Marisa V.; Fernandes, Pedro; Fonseca, Luís Lopes; Fonseca, César; Ferreira, Frederico Castelo

The pentose-assimilating capacity of yeasts from the genus Pseudozyma, P. antarctica PYCC 5048T, P.aphidis PYCC 5535Tand P. rugulosa PYCC 5537T, was exploited towards the production of mannosylery-thritol lipids (MEL), a glycolipid with biosurfactant properties. The three strains tested were able togrow on d-xylose and l-arabinose with similar maximum specific growth rates to those estimated on d-glucose (aroun...

Date: 2014   |   Origin: Repositório do LNEG

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