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Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cacha...

Cunha, Aureliano C.; Santos, Renato A Corrêa Dos; Riaño-Pachon, Diego M.; Squina, Fábio M.; Oliveira, Juliana V. C.; Goldman, Gustavo H.

Wickerhamomyces anomalus LBCM1105 is a yeast isolated from cachaça distillery fermentation vats, notable for exceptional glycerol consumption ability. We report its draft genome with 20.5x in-depth coverage and around 90% extension and completeness. It harbors the sequences of proteins involved in glycerol transport and metabolism.


High-affinity transport, cyanide-resistant respiration, and ethanol production ...

da Cunha, Aureliano Claret; Gomes, Lorena Soares; Godoy-Santos, Fernanda; Faria-Oliveira, Fábio; Teixeira, Janaína Aparecida

Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus g...


Methodologies to generate, extract, purify and fractionate yeast ECM for analyt...

Faria-Oliveira, Fábio; Carvalho, Joana; Belmiro, Celso L R; Martinez-Gomariz, Montserrat; Hernaez, Maria Luisa; Pavão, Mauro; Gil, Concha

In a multicellular organism, the extracellular matrix (ECM) provides a cell-supporting scaffold and helps maintaining the biophysical integrity of tissues and organs. At the same time it plays crucial roles in cellular communication and signalling, with implications in spatial organisation, motility and differentiation. Similarly, the presence of an ECM-like extracellular polymeric substance is known to support...


Yeasts, the man’s best friend

Tulha, Joana; Carvalho, Joana; Armada, Rui; Faria-Oliveira, Fábio; Lucas, Cândida; Pais, Célia; Almeida, M. J.; Ferreira, Célia

1. Introduction In most cultures, bread making depends on a fermentation step. The flour leavening ability was, at first, most probably dependent on spontaneous fermentation. It became a controlled process by the maintenance of fresh innocula from one preparation to the next and this kind of environmental constraints eventually generated a particular type of yeast and bacteria biodiversity, adapted to ferment a...


Programmed cell death processes in Saccharomyces cerevisiae are altered by GUP1...

Tulha, Joana; Faria-Oliveira, Fábio; Lucas, Cândida; Ferreira, Célia

During the past years, yeast has been successfully established as a model to study mechanisms of programmed cell death regulation. Saccharomyces cerevisiae commits to cell death showing typical hallmarks of metazoan apoptosis, in response to different stimuli. Gup1p, an O-acyltransferase, is required for several cellular processes that are related to apoptosis development, such as rafts integrity and stability,...


Molecular characterization of Saccharomyces cerevisiae extracellular matrix and...

Carvalho, Joana; Faria-Oliveira, Fábio; Ferreria, Célia; Lucas, Cândida

Yeast, Saccharomyces cerevisiae, presents itself as the best studied eukaryotic model, with the possibility to modify its genetic information with highly advanced molecular techniques. This microorganism displays a high degree of similarity and conservation of processes with higher eukaryotes. Several fundamental mechanisms such as cell cycle regulation, DNA replication, recombination and repair were first unco...


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