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The draft genome sequence of cork oak

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...


The draft genome sequence of cork oak

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...


Enzymatic properties, evidence for in vivo expression, and intracellular locali...

Leal, Ana Rita; Cruz, Rui; Bur, Daniel; Huesgen, Pitter F; Faro, Rosário; Manadas, Bruno; Wlodawer, Alexander; Faro, Carlos; Simões, Isaura

The widespread presence of pepsin-like enzymes in eukaryotes together with their relevance in the control of multiple biological processes is reflected in the large number of studies published so far for this family of enzymes. By contrast, pepsin homologs from bacteria have only recently started to be characterized. The work with recombinant shewasin A from Shewanella amazonensis provided the first documentati...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria; Almeida, Paulo; Almeida, Tânia; Amorim, Maria; Araújo, Susana

Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork ...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tânia; Amorim, Maria Isabel; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, Jose B.; Abreu, Isabel A.; Alabca, Claudia S.; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tania; Amorim, Maria Isabel; Araujo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...

Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, Susana

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...


Self-assembled Mannan Nanogel: Cytocompatibility and Cell Localization

Carvalho, Vera; Castanheira, Pedro; Madureira, Pedro; Ferreira, Sílvia A.; Costa, Carla; Teixeira, João Paulo; Faro, Carlos; Vilanova, Manuel

Amphiphilic mannan, produced by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, self-assembles in aqueous medium through hydrophobic interactions among alkyl chains. Resultant nanogel is stable, spherical, polydisperse, with 50-140 nm mean hydrodynamic diameter depending on the polymer degree of substitution, and nearly neutral negative surface charge. No cytotoxicity of man...


Self-assembled dextrin nanogel as protein carrier: controlled release and biolo...

Carvalho, Vera; Castanheira, Pedro; Madureira, Pedro; Ferreira, Sílvia A.; Costa, Carlos M.; Faro, Carlos; Teixeira, João P.; Vilanova, Manuel

Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems for its effective delivery. In previous work, it has been shown that a dextrin nanogel effectively incorporated and stabilized rIL10, enabling its release over time. In this work, the delivery system...


Recombinant laforin for structural studies

Castanheira, Pedro; Moreira, Susana Margarida Gomes; Casal, Margarida; Gama, F. M.; Faro, Carlos


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