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Testis transcriptomic analyses reveal the effects of an algae feed on sperm qua...
Félix de Azeredo Pinto e Melo, Francisca; Raposo de Magalhães, Cláudia; Marrero Alemán, Carlos; Duarte, Daniel Filipe Correia; Parente, PedroThe usage of dietary algae antioxidants to improve fish reproduction is under-explored, especially in terms of the male reproductive system. In this experiment, 6 % of a blended meal of Phaeodactylum tricornutum and Gracilaria gracilis was incorporated in Senegalese sole broodstock feed, to evaluate the effects on sperm quality of F1 males throughout the breeding season. For that, two groups of breeders were fe...
The use of egg quality parameters to evaluate the effect of a diet supplemented...
Ramos Júdez, Sandra; Fatsini Fernández, Elvira; Marrero Alemán, Carlos; García-Pichel, Candela; Parente, Pedro; Medina, D.; Castro, C.; Cabrita, ElsaEnhancing egg quality can be achieved by improving breeders’ diet through the antioxidant content, as oxidative stress could adversely affect egg quality. Micro- and macroalgae species are natural sources of antioxidants and other essential nutrients that can be incorporated in fish feeds. In this study the quality of Scophthalmus maximus eggs were compared between breeders fed a commercial (non-supplemented) d...
Cryopreservation did not affect spermatogonia global methylation profile in Sen...
Almeida, Maria Mafalda; Cabrita, Elsa; Laizé, Vincent; Brionne, Aurélien; Labbé, Catherine; Fatsini Fernández, ElviraSpermatogonia cryopreservation is a method to preserve valuable genomes from both maternal and paternal origin. The damage associated with the application of this technology on post-thaw cell quality is important to assess, including at the epigenetic level. This study aimed to assess post-thawed spermatogonia quality by evaluating alterations in plasma membrane integrity, DNA integrity (fragmentation and apopt...
Comparative transcriptome analysis reveals molecular damage associated with cry...
Anjos, Catarina; Duarte, Daniel Filipe Correia; Fatsini Fernández, Elvira; Domitília Matias; Cabrita, ElsaBackground The Portuguese oyster Crassostrea angulata, a bivalve of significant economic and ecological importance, has faced a decline in both production and natural populations due to pathologies, climate change, and anthropogenic factors. To safeguard its genetic diversity and improve reproductive management, cryopreservation emerges as a valuable strategy. However, the cryopreservation methodologies lead to...
Cutting-Edge methods in Teleost and Chondrichthyan reproductive biology
Mechaly, Alejandro S.; Awruch, Cynthia; Cabrita, Elsa; Costas, Benjamín; Fernandes, Jorge M.O.; Gallego, Victor; Hirt-Chabbert, JorgeReproduction is a fundamental biological process for producing offspring and ensuring the continuation of the species. With nearly 35,000 fish species worldwide exhibiting a very diverse range of reproductive systems and strategies, the study of fish reproduction is a significant challenge. Research into various fish reproductive models is driven by their use in human biomedical applications, improving the dive...
Comparative transcriptome analysis reveals molecular damage associated with cry...
Anjos, Catarina; Duarte, Daniel Filipe Correia; Fatsini Fernández, Elvira; Matias, Domitília; Cabrita, ElsaThe Portuguese oyster Crassostrea angulata, a bivalve of significant economic and ecological importance, has faced a decline in both production and natural populations due to pathologies, climate change, and anthropogenic factors. To safeguard its genetic diversity and improve reproductive management, cryopreservation emerges as a valuable strategy. However, the cryopreservation methodologies lead to some damag...