5 documents found, page 1 of 1
Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PN...
Bárria, Cátia; Athayde, Diogo; Hernandez, Guillem; Fonseca, Leonor; Casinhas, Jorge; Cordeiro, Tiago N.; Archer, Margarida; Arraiano, Cecília M.Funding Information: This work was funded by FCT - Fundação para a Ciência e a Tecnologia, I. P., through projects MOSTMICRO-ITQB (UIDB/04612/2020 and UIDP/04612/2020), LS4FUTURE Associated Laboratory-LA/P/0087/2020, and project PTDC/BIA-BQM/28479/2017 to RGM. CB was financed by a contract under the project PTDC/BIA-BQM/28479/2017. DA and GH have a PhD fellowship from FCT with the references PD/BD/147987/2019 a...
Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids
Alves, Márcia M. S.; Melo, Manuel N.; Mertens, Haydyn D. T.; Pereiro, Ana B.; Archer, MargaridaSynchrotron SAXS data were collected at beamline P12 operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Publisher Copyright: © 2023 by the authors.; Proteins are inherently unstable, which limits their use as therapeutic agents. However, the use of biocompatible cosolvents or surfactants can help to circumvent this problem through the stabilization of intramolecular and solvent-med...
Secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to...
Gonçalves, Juliana; Juliano, A. Margarida; Charepe, Nádia; Alenquer, Marta; Athayde, Diogo; Ferreira, Filipe; Archer, Margarida; Amorim, Maria JoãoFunding Information: We are very grateful to all study participants. We thank Cl?udia Andrade (CEDOC Flow Cytometry Platform), Manuela Correia, Yasmina Orraca, Daniela Silva, and Jos? Ramalho for support. This work was supported by European Society of Clinical Microbiology and Infectious Diseases (ESCMID) and by Gilead G?nese (PGG/009/2017) grants to H.S. RESEARCH 4 COVID-19 to M.J.A. European Union (EU) Horizo...
A structure-based catalytic mechanism for the xanthine oxidase family of molybd...
Huber, Robert; Hof, Peter; Duarte, Rui O.; Moura, Jose J. G.; Moura, Isabel; Liu, Ming Yih; LeGall, Jean; Hille, Russ; Archer, MargaridaThe crystal structure of the xanthine oxidase-related molybdenum-iron protein aldehyde oxidoreductase from the sulfate reducing anaerobic Gram- negative bacterium Desulfovibrio gigas (Mop) was analyzed in its desulfo-, sulfo-, oxidized, reduced, and alcohol-bound forms at 1.8-Å resolution. In the sulfo-form the molybdenum molybdopterin cytosine dinucleotide cofactor has a dithiolenebound fac-[Mo, =O, =S, ···(OH...
Subunit composition, crystallization and preliminary crystallographic studies o...
Romão, Maria J.; Barata, Belarmino A. S.; Archer, Margarida; Lobeck, Karin; Moura, Isabel; CARRONDO, Maria A.; LeGall, Jean; Lottspeich, FriedrichThe Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe‐2S] centers. The enzyme was characterized by SDS/PAGE, gel‐filtration and analytical ultracentrifugation experiments. It was crystallized at 4°C, pH 7.2, using isopropanol and MgCl 2 as precipitants. The crystals diffract beyond 0.3‐nm (3.0‐Å) resolution and belong to space group P6 1 22 or its enantiomorph, ...