56 documents found, page 1 of 6
Animal models of colorectal cancer: from spontaneous to genetically engineered ...
Nascimento-Gonçalves, Elisabete; Mendes, Bruno; Silva-Reis, Rita; Faustino-Rocha, Ana Isabel; Gama, Adelina; Oliveira, Paula AlexandraColorectal cancer is one of the most common gastrointestinal malignancies in humans, affecting approximately 1.8 million people worldwide. This disease has a major social impact and high treatment costs. Animal models allow us to understand and follow the colon cancer progression; thus, in vivo studies are essential to improve and discover new ways of prevention and treatment. Dietary natural products have been...
Animal models in pharmacology: a brief history awarding the Nobel Prizes for Ph...
Jota Baptista, Catarina; Faustino-Rocha, Ana Isabel; Oliveira, Paula AlexandraBackground: The Nobel Prize of Physiology or Medicine (NPPM) has recognized the work of 222 scientists from different nationalities, from 1901 until 2020. From the total, 186 award researchers used animal models in their projects, and 21 were attributed to scientists and projects directly related to Pharmacology. In the most recent years, genetics is a dominant scientific area, while at the beginning of the 20t...
Refinement of animal model of colorectal carcinogenesis through the definition ...
Silva-Reis, Rita; Faustino-Rocha, Ana Isabel; Gonçalves, Mariana; Castro Ribeiro, Catarina; Ferreira, Tiago; Ribeiro-Silva, Carla; Gonçalves, LioThis study aimed to define appropriate humane endpoints (HEs) for an animal model of colorectal carcinogenesis (CRC). Twenty-nine male Wistar rats were divided into two control groups (CTRL1 and CTRL2) injected with ethylenediamine tetraacetic acid (EDTA)–saline solutions and two induced groups (CRC1 and CRC2) injected with 1,2-dimethylhydrazine (DMH) for seven weeks. A score sheet with 14 biological parameters...
Pre-neoplastic lesions associated with liver and colon responses to 1,2-dimethy...
Silva-Reis, Rita; Faustino-Rocha, Ana Isabel; Castro-Ribeiro, Carla; Gonçalves, Mariana; Ferreira, Tiago; Gama, Adelina; Ferreira, RitaBackground: The high incidence and mortality of colorectal cancer (CRC) combined with the lack of an effective method for early diagnosis and effective treatments make CRC one of the most relevant cancers to be studied. Thus, our work aims to study the spectrum of liver and colon lesions induced in rats by the 1,2-Dimethylhydrazine (DMH). Materials and Methods: Twenty-nine male Wistar rats were randomly divided...
The influence of physical exercise on oestrogen and androgen receptor expressio...
Nascimento-Gonçalves, Elisabete; Seixas, Fernanda; Faustino-Rocha, Ana Isabel; Pires, Maria João; Neuparth, Maria João; Colaço, Bruno; Ferreira, RitaBackground: Oestrogen (ER) and androgen (AR) recep- tors play an important role in normal prostate development and are also implied in prostate cancer (PCa) development. Several studies suggested that physical activity may decrease the risk of PCa development and also changes sexual hor- mones and their receptors. This study aimed to evaluate the effects of physical exercise on ERα and AR expression in a rat mo...
Effects of physical exercise in biochemical parameters and dorsolateral prostat...
Nascimento-Gonçalves, Elisabete; Seixas, Fernanda; Faustino-Rocha, Ana Isabel; Pires, Maria João; Neuparth, Maria João; Moreira-Gonçalves, DanielBackground: Prostate cancer (PCa) is among the most prevalent cancers worldwide. Physical exercise is widely recognized due to its beneficial effects. This study aimed to evaluate the effects of physical exercise on biochemical pa- rameters and in dorsolateral prostate lesions in a rat model of PCa. Materials and Methods: Ninety-five male Wistar Unilever rats were randomly divided into eight groups sacrificed a...
Rat prostate: practical tips for ultrasonographic monitoring
Faustino-Rocha, Ana Isabel; Ferreira, Rita; Pires, Maria João; Fardilha, Margarida; Oliveira, Paula Alexandra; Ginja, MárioBackground: Prostate is the largest accessory gland of the male reproductive tract. The prostate of men over 40 years- old is frequently affected by several pathologies, like benign prostate hyperplasia and cancer. Rats have been used as model to study prostate cancer. This study intended to address the usefulness of ultrasonography for rat prostate monitoring. Materials and Methods: Eight male Wistar Unilever ...
Immunology and mammary cancer development: addressing the role of mast cells
Faustino-Rocha, Ana Isabel; Oliveira, Paula Alexandra; Gama, Adelina; Ferreira, Rita; Ginja, MárioBackground: Mammary cancer is one of the most frequent cancers worldwide. Mast cells are among the cells of tumor microenvironment and have been associated with increased angiogenesis and poor prognosis. Despite this, the role of mast cells on mammary cancer is not fully elucidated. In this way, this work studied the role of mast cells in a rat model of mammary cancer chemically-induced. Materials and Methods: ...
Rodent models: what is their importance for cancer study?
Faustino-Rocha, Ana IsabelCancer is one of the most frequent diseases worldwide, accounting for approximately 10 million deaths in 2020. The most common cancers in 2020 were: breast, lung, colon and rectum, prostate, skin, and stomach. Cancer arises from the conversion of normal cells into initiated cells as a result of the interaction between intrinsic (genetic) and extrinsic factors, namely physical agents (ultraviolet radiation), che...
Ganoderma lucidum effects in an animal model of obesity
Castro-Ribeiro, Carla; Silva-Reis, Rita; Mendes-Gonçalves, Mariana; Ferreira, Rita; Taofiq, O; Barros, L; Martins, Tânia; Faustino-Rocha, Ana IsabelObesity is a pandemic disease, and its evolution can be influenced by regular consumption of natural bioactive compounds. Mushrooms, such as Ganoderma lucidum (GL), include a wide variety of biomolecules with potential anti-obesity effects. This work aimed to study the influence of GL in an animal model of obesity. Forty-eight male mice (C57BL/6J) were divided into 5 groups: Group (G)-1-Western Control Diet; G-...