4 documents found, page 1 of 1
Analysis of the effect of increased a2,3-sialylation on RTK activation in MKN45...
Balmaña, M; Diniz, F; Feijão, T; Barrias, CC; Mereiter, S; Reis, CAIn the scenario of personalized medicine, targeted therapies are currently the focus of cancer drug development. These drugs can block the growth and spread of tumor cells by interfering with key molecules involved in malignancy, such as receptor tyrosine kinases (RTKs). MET and Recepteur d’Origine Nantais (RON), which are RTKs frequently overactivated in gastric cancer, are glycoprotein receptors whose activat...
Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype ...
Balmaña, M; Mereiter, S; Diniz, F; Feijão, T; Barrias, CC; Reis, CACellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell⁻cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features, an innovative high-throughput 3D spheroid culture methodology has been developed for gastric cancer cells. The ass...
Xeno-free pre-vascularized spheroids for therapeutic applications
Bauman, E; Feijão, T; Carvalho, DTO; Granja, PL; Barrias, CCSpheroid culture has gained increasing popularity, arising as a promising tool for regenerative medicine applications. Importantly, spheroids may present advantages over single-cell suspensions in cell-based therapies (CT). Unfortunately, most growth media used for spheroid culture contain animal origin-components, such as fetal bovine serum (FBS). The presence of FBS compromises the safety of CT and presents e...
Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-m...
Reis, R; Feijão, T; Gouveia, S; Pereira, AJ; Matos, I; Sampaio, P; Maiato, H; Sunkel, CEEstablishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein Mast/Orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that Mast accumulates at kinetochores early in mitosis, which is followed by a polewar...