5 documents found, page 1 of 1
Lysine-PEGylated Cytochrome C with Enhanced Shelf-Life Stability
Santos, João H. P. M.; Feitosa, Valker A.; Meneguetti, Giovanna P.; Carretero, Gustavo; Coutinho, João A. P.; Ventura, Sónia P. M.Cytochrome c (Cyt-c), a small mitochondrial electron transport heme protein, has been employed in bioelectrochemical and therapeutic applications. However, its potential as both a biosensor and anticancer drug is significantly impaired due to poor long-term and thermal stability. To overcome these drawbacks, we developed a site-specific PEGylation protocol for Cyt-c. The PEG derivative used was a 5 kDa mPEG-NHS...
Building better biobetters: from fundamentals to industrial application
Torres-Obreque, Karin M.; Meneguetti, Giovanna P.; Muso-Cachumba, Jorge J.; Feitosa, Valker A.; Santos, João H. P. M.; Ventura, Sónia P. M.Biological drugs or biopharmaceuticals off patent open a large market for biosimilars and biobetters, follow-on biologics. Biobetters, in particular, are new drugs designed from existing ones with improved properties such as higher selectivity, stability, half-life and/or lower toxicity/immunogenicity. Glycosylation is one of the most used strategies to improve biological drugs, nonetheless bioconjugation is an...
Laccase production in bioreactor scale under saline condition by the marine-der...
Mainardi, Pedro H. [UNESP]; Feitosa, Valker A.; Brenelli de Paiva, Livia B.; Bonugli-Santos, Rafaella C.; Squina, Fabio M.; Pessoa, AdalbertoMade available in DSpace on 2018-12-11T16:52:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-05-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Laccase production in saline conditions is still poorly studied. The aim of the present study was to investigate the production of laccase in two different types of bioreactors by the marine-derived basidiomycete Peniophora sp. CBMAI 1063. T...
Biomolecules extracted by ATPs: practical examples
Molino, João Vitor Dutra; Feitosa, Valker A.; Novaes, Leticia Celia de Lencastre; Ebinuma, Valéria de Carvalho Santos [UNESP]; Lopes, André MoreniMade available in DSpace on 2016-01-28T16:55:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2016-01-28T17:02:28Z : No. of bitstreams: 1 ISSN1665-2738-2014-13-02-359-377.pdf: 4166382 bytes, checksum: 4c8c484bf7cceeef47bb923038908503 (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselh...
Marine-derived fungi: diversity of enzymes and biotechnological applications
Bonugli-Santos, Rafaella C.; Santos Vasconcelos, Maria R. dos; Passarini, Michel R. Z.; Vieira, Gabriela A. L. [UNESP]; Lopes, Viviane C. P. [UNESP]Made available in DSpace on 2015-10-21T13:12:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-04-10. Added 1 bitstream(s) on 2015-10-22T09:52:03Z : No. of bitstreams: 1 WOS000352554600001.pdf: 1118395 bytes, checksum: 70c7a91dc67371d61cddaa5be88b73bc (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de...