7 documents found, page 1 of 1
A portable and low-cost optical device for pigment-based taxonomic classificati...
Magalhães, Vitor; Pinto, Vânia Cristina Gonçalves; Sousa, Paulo Jorge Teixeira; Afonso, José A.; Gonçalves, L. M.; Fernández, Emilio; Minas, GraçaThe proliferation of certain phytoplankton species may lead to harmful algal blooms (HABs) that can affect living resources and human health. Therefore, an accurate identification of phytoplankton populations is essential for the sustainable management of some activities relevant for the blue economy, such as aquaculture, being also relevant for environmental monitoring and marine research purposes. Microalgae ...
Portable and low-cost fluorometer for phytoplankton monitoring
Sá, Leonardo Rafael Correia; Pinto, Vânia Cristina Gonçalves; Sousa, Paulo Jorge Teixeira; Magalhães, Vitor H.; Carvalho, Denise Andreia MotaPhytoplankton monitoring using fluorescence spectroscopy techniques has been frequently used over time. This monitoring is based on the detection of the main photosynthetic pigment, chlorophyll-a, as its concentration is widely used as a proxy of phytoplankton biomass in the water column. However, the fluorometers available for their measurement and use in the field are still quite expensive and bulky, which li...
A microfluidic device for size-based microplastics and microalgae separation
Magalhães, Vitor H.; Pinto, Vânia Cristina Gonçalves; Sousa, Paulo Jorge Teixeira; Gonçalves, L. M.; Fernández, Emilio; Minas, GraçaPhytoplankton are microscopic marine algae that constitute the foundation of the aquatic food web. They are essential to drive life in the oceans but can also be harmful in the form of algae blooms, either by producing phytoplankton toxins or massive biomass proliferation. Microalgae cell size is a relevant morphologic trait that can help identifying species responsible for these blooms at early stages. Sorting...
Design and in situ validation of low-cost and easy to apply anti-biofouling tec...
Matos, Tiago; Pinto, Vânia; Sousa, Paulo; Martins, Marcos; Fernández, Emilio; Henriques, Renato F.; Gonçalves, Luis MiguelBiofouling is the major factor that limits long-term monitoring studies with automated optical instruments. Protection of the sensing areas, surfaces, and structural housing of the sensors must be considered to deliver reliable data without the need for cleaning or maintenance. In this work, we present the design and field validation of different techniques for biofouling protection based on different housing m...
Spiral inertial microfluidics for separation and concentration of phytoplankton
Magalhães, Vitor; Pinto, Vânia Cristina Gonçalves; Sousa, Paulo; Gonçalves, Luís; Fernández, Emilio; Minas, GraçaHarmful algal blooms (HABs) are a recurring phenomenon along all continents, posing a global threat, particularly due to shellfish poisoning and public health, and their timely monitoring is vital for safeguarding the blue economy. The ability to efficiently isolate species of interest, such as harmful dinoflagellates, is a key process for HABs monitoring which is particularly complex because of the typically h...
Methodology for phytoplankton taxonomic group identification towards the develo...
Carvalho, Denise Andreia Mota; Pinto, Vânia Cristina Gonçalves; Sousa, Paulo Jorge Teixeira; Magalhães, Vitor H.; Fernández, Emilio; Gomes, Pedro A.This paper presents the absorbance and fluorescence optical properties of various phytoplankton species, looking to achieve an accurate method to detect and identify a number of phytoplankton taxonomic groups. The methodology to select the excitation and detection wavelengths that results in superior identification of phytoplankton is reported. The macroscopic analyses and the implemented methodology are the ba...
Nitrogen isotope signature evidences ammonium deprotonation as a common transpo...
Ariz, Idoia; Boeckstaens, Mélanie; Gouveia, Catarina; Martins, Ana Paula; Sanz-Luque, Emanuel; Fernández, Emilio; Soveral, Graça; von Wirén, NicolausAmmonium is an important nitrogen (N) source for living organisms, a key metabolite for pH control, and a potent cytotoxic compound. Ammonium is transported by the widespread AMT-Mep-Rh membrane proteins, and despite their significance in physiological processes, the nature of substrate translocation (NH3/NH4+) by the distinct members of this family is still a matter of controversy. Using Saccharomyces cerevisi...