16 documents found, page 1 of 2
Fluorometric Liposome Screen for Inhibitors of a Physiologically Important Bact...
Fernandes, AS; Pombinho, AR; Teixeira-Duarte, CM; Morais-Cabral, JH; Harley, CAThe bacterial K+ homeostasis machinery is widely conserved across bacterial species, and different from that in animals. Dysfunction in components of the machinery has an impact on intracellular turgor, membrane potential, adaptation to changes in both extracellular pH and osmolarity, and in virulence. Using a fluorescence-based liposome flux assay, we have performed a high-throughput screen to identify novel i...
Changes in the biophysical properties of the cell membrane are involved in the ...
Santos, F; Lobo, G; Fernandes, AS; Videira, A; Almeida, RNeurospora crassa is a non-pathogenic filamentous fungus widely used as a multicellular eukaryotic model. Recently, the biophysical properties of the plasma membrane of N. crassa conidia were thoroughly characterized. They evolve during conidial germination at a speed that depends on culture conditions, suggesting an important association between membrane remodeling and the intense membrane biogenesis that take...
Enhancement of hERG channel activity by scFv antibody fragments targeted to the...
Harley, CA; Starek, G; Jones, DK; Fernandes, AS; Robertson, GA; Morais-Cabral, JHThe human human ether-à-go-go-related gene (hERG) potassium channel plays a critical role in the repolarization of the cardiac action potential. Changes in hERG channel function underlie long QT syndrome (LQTS) and are associated with cardiac arrhythmias and sudden death. A striking feature of this channel and KCNH channels in general is the presence of an N-terminal Per-Arnt-Sim (PAS) domain. In other proteins...
Molecular insights into the mechanism of calmodulin inhibition of the EAG1 pota...
Marques-Carvalho, MJ; Oppermann, J; Muñoz, E; Fernandes, AS; Gabant, G; Cadene, M; Heinemann, SH; Schönherr, R; Morais-Cabral, JHThe human EAG1 potassium channel belongs to the superfamily of KCNH voltage-gated potassium channels that have roles in cardiac repolarization and neuronal excitability. EAG1 is strongly inhibited by Ca2+/calmodulin (CaM) through a mechanism that is not understood. We determined the binding properties of CaM with each one of three previously identified binding sites (BDN, BDC1, and BDC2), analyzed binding to pr...
Violência sobre Profissionais de Saúde...Que Implicações Psicológicas?
Fernandes, ASA violência sobre os profissionais de saúde é uma problemática crescente nos dias de hoje. Deste modo, os enfermeiros que diariamente estão expostos a estas situações, muitas vezes não sabem lidar com elas, tornando-se assim premente estudar e analisar este fenómeno. Este artigo de reflexão desenvolve-se na sequência do trabalho final realizado no âmbito do III Curso de Pós-Graduação em Estratégias e Intervençã...
Macrocyclic copper(II) complexes as superoxide scavengers
Matxain, JM; Santos, DJVAdos; Fernandes, AS; Cabral, MF; Costa, J; Castro, M; Oliveira, NG; Guedes, R
Evaluation of the protective effect of superoxide scavengers on the cytotoxicit...
Fernandes, AS; Gaspar, J; Cabral, MF; Serejo, J; Bettencourt, A; Rueff, J; Castro, M; Costa, J; Oliveira, NG
Evaluation of methylmethacrylate leaching from acrylic bone cement
Bettencourt, AF; Pinheiro, L; Calado, A; Fernandes, AS; Oliveira, NG; Monteiro, J; Castro, M
Pyridine-containing macrocyclic copper (II) complexes
Fernandes, AS; Oliveira, N; Gaspar, JF; Cabral, MF; Caneiras, C; Rueff, J; Castro, M; Costa, J
ac3Py[14]ene
Caneiras, C; Fernandes, AS; Cabral, MF; Oliveira, N; Costa, J; Castro, M; Duarte, A