9 documents found, page 1 of 1
A comprehensive review of mammalian pigmentation: paving the way for innovative...
Fernandes, Bruno Pacheco; Cavaco-Paulo, Artur; Matamá, Maria Teresa Gonçalves MacedoThe natural colour of hair shafts is formed at the bulb of hair follicles, and it is coupled to the hair growth cycle. Three critical processes must happen for efficient pigmentation: (1) melanosome biogenesis in neural crest-derived melanocytes, (2) the biochemical synthesis of melanins (melanogenesis) inside melanosomes, and (3) the transfer of melanin granules to surrounding pre-cortical keratinocytes for th...
Composition for hair follicle modulation, methods and uses thereof
Cavaco-Paulo, Artur; Matamá, Teresa; Costa, Cristiana C.; Fernandes, Bruno PachecoThe present disclosure relates to a composition for use in the hair follicle modulation comprising a bioactive agent a thickener agent; a preservative; and a denaturing alcohol. The present disclosure also comprises a pharmaceutical composition for use in the prevention, therapy or treatment of abnormal defective production of melanin, comprising a bioactive agent selected from dipyridamole, dipyridamole salt, ...
Comparing the delivery to the hair bulb of two fluorescent molecules of distinc...
Costa, Cristiana; Fernandes, Bruno Pacheco; Guimarães, Diana Isabel Pereira; Nogueira, Eugénia; Martins, Madalena; Matamá, Maria Teresa Gonçalves MacedoThe follicular route is an important drug penetration pathway in any topical application, either concerning dermatological and cosmetic skin treatments or any transdermal administration regimen. Efficient transport into follicles will depend on drug inherent properties but also on the chosen vehicle. The main study goal was to compare several systems for the delivery to the hair bulb of two fluorescent molecule...
Modification of the human hair: modulators of melanogenesis as agents of colour...
Fernandes, Bruno PachecoA cor do cabelo é um elemento marcante do nosso visual e tem um impacto social irrefutável. Em virtude da importância dada ao aspeto físico, a coloração do cabelo é hoje uma prática bastante comum. Todavia, os efeitos adversos do recurso frequente a tais práticas exigem que novos métodos sejam desenvolvidos para alteração da cor do cabelo. Neste sentido, esta tese teve como objetivo principal demonstrar que, in...
Cyclosporin A-loaded poly(d,l-lactide) nanoparticles: a promising tool for trea...
Fernandes, Bruno Pacheco; Matamá, Teresa; Gomes, Andreia C; Cavaco-Paulo, ArturBackground: Alopecia treatments are scarce and lack efficacy. Cyclosporin A (CsA) has hair growth-inducing properties but its poor cutaneous absorption undermines its use in topical treatments. Aim: Development of a new potential topical treatment of alopecia with CsA. Materials \& methods: CsA-loaded poly(d,l-lactide) (PLA) nanoparticles were obtained and characterized. Skin permeation was evaluated in ex vivo...
Fluorescent quantification of melanin
Fernandes, Bruno Pacheco; Matamá, Maria Teresa; Guimarães, Diana Isabel Pereira; Gomes, Andreia C; Cavaco-Paulo, ArturMelanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic con...
Improved Poly (D,L-lactide) nanoparticles-based formulation for hair follicle t...
Fernandes, Bruno Pacheco; Silva, R.; Ribeiro, Artur J.; Matamá, Maria Teresa; Gomes, Andreia C; Paulo, Artur CavacoObjective Hair follicles are widely recognized as the preferential target and site of accumulation for nanoparticles after topical application. This feature is of particular importance for hair cosmetics, having the potential to refine the treatment of several hair follicle-related disorders. The aim of this work was to improve the preparation of Poly (D,L-lactide) (PLA) nanoparticles for in vivo follicular tar...
Development and characterization of PLA nanoparticles as carriers for topical d...
Fernandes, Bruno Pacheco; Silva, Raquel; Gomes, Andreia C; Paulo, Artur CavacoNanoparticles are seen today as one of the best approaches for the delivery of drugs into the skin. Poly (Lactic Acid) (PLA) is biocompatible and biodegradable and already approved for clinical use. Thus, this work aimed to study the effect of several parameters on the properties of PLA nanoparticles (PLA-NPs) intended for topical delivery. The yield of nanoparticles formation and entrapment efficiencies of lip...
Preparation of particles for cosmetic applications in human hair
Fernandes, Bruno PachecoNanoparticles (NPs) have a huge interest for transdermal applications, demonstrating their ability to be trapped in the hair follicles (HFs) where they release the entrapped compounds. For hair cosmetics, this discovery is particularly important as it can improve the treatment of several hair follicle related disorders/diseases. Therefore, the aim of this work was to obtain NPs of Poly (Lactic Acid) (PLA-NPs) f...