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Antimicrobial and anti-adhesive activities of cell-bound biosurfactant from Lac...

In this work, biosurfactant production by different Lactobacillus strains was studied using the conventional MRS medium for lactic acid bacteria. Lactobacillus agilis CCUG31450 produced a cell-bound biosurfactant that reduced the surface tension of water to 42.5 mN/m, and exhibited a high emulsifying activity (E24=60%). The amount of biosurfactant produced was 84 mg/l, with a cmc of 7.5 mg/ml. A preliminary che...

A bacteriophage detection tool for viability assessment of Salmonella cells

Salmonellosis, one of the most common food and water-borne diseases, has a major global health and economic impact. Salmonella cells present high infection rates, persistence over inauspicious conditions and the potential to preserve virulence in dormant states when cells are viable but non-culturable (VBNC). These facts are challenging for current detection methods. Culture methods lack the capacity to detect ...

Spintronic devices for biomedical applications

Spintronic devices have been proposed over the past decade for various biomedical applications. These include static or dynamic biomolecular recognition platforms ( DNA-cDNA, antibody-antigen, phage-bacteria, ), cytometer and cell separation devices and lateral bio assay platforms , microelectrode based devices for neuroelectronic applications, and hybrid sensor arrays for imaging applications [1]. The biomolec...

Peptides and derivatives thereof for detection and control of Salmonella

The present subject matter relates to isolated polypeptide having one of the sequences SEQ. ID. No 8, 9, 10, 11, 12, 13, 14 or fragments or derivatives thereof, having the ability to recognize and bind to a broad range of Salmonella strains. Moreover, the present subject matter further relates to nucleic acid molecules having one of the SEQ. ID. No 1, 2, 3, 4, 5, 6, 7 encoding said recombinant polypeptide or fr...

Challenges in spintronic platforms for biomedical applications

Integrated spintronic biochip platforms are being developed for portable, point-of-care, diagnostic and cytometric applications [1,2]. Hybrid systems incorporating magnetoresistive sensors are applied to neuroelectronic studies and biomedical imaging, namely magnetoencephalography and magnetocardiography. Lab-on-a-chip MR-based platforms are under development to perform biological studies at the single molecule...

Development of a phage-based biosensor to detect Salmonella in food stuff

Food- and waterborne illnesses are a serious public health concern worldwide and have stimulated research aiming at a rapid and accurate detection of pathogens by applying biosensing technologies. Salmonella, Campylobacter and E. coli are some examples of pathogens that have an enormous impact on public health. Many publications have mentioned different type of biosensors for a broad range of bacteria. These me...

Model interface for pathogens detection systems

Development of a phage-based biosensor to detect salmonella in food stuff

Method for bacteriophage isolation against target Campylobacter strains

Aims: Poultry meat is considered a major source of Campylobacter. This micro-aerobic bacterium is commonly responsible for foodborne illness. This work focuses on the isolation of Campylobacter coli lytic bacteriophages (phages) against target C. coli strains. Methods and Results: A method involving the enrichment of free-range chicken samples in a broth containing the target C. coli strains and salts (CaCl2 an...

The use of bacteriophage endolysins as antibacterial compounds