Encontrados 22 documentos, a visualizar página 1 de 3
Extracellular vesicles enriched with an endothelial cell pro-survival microRNA ...
Fernandes, Hugo; Zonnari, Alessandra; Abreu, Ricardo Cerqueira de; Aday, Sezin; Barão, Marta; Albino, Inês; Lino, Miguel; Branco, Ana; Seabra, CátiaEndothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro, we showed that miR-425-5p, ...
Influence of nitric oxide and phosphodiesterases during in vitro maturation of ...
Botigelli, Ramon Cesar; Schwarz, Katia Lancellotti; Zaffalon, Fabiane Gilli; Del Collado, Maite; Castro, Fernanda Cavallari; Fernandes, HugoMade available in DSpace on 2022-04-29T08:45:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-06-01; This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the solubl...
Effect of follicular fluid supplementation during in vitro maturation on total ...
Cruz, Maria Helena Coelho [UNESP]; Saraiva, Naiara Zoccal [UNESP]; da Cruz, Jurandir Ferreira; Oliveira, Clara Slade [UNESP]; Del Collado, Maite [UNESP]Made available in DSpace on 2022-04-29T07:14:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01; This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to me...
Exogenous loading of miRNAs into small extracellular vesicles
Abreu, Ricardo C de; Ramos, Cristiana V; Becher, Clarissa; Lino, Miguel; Jesus, Carlos; Martins, Paula A da Costa; Martins, Patrícia A TSmall extracellular vesicles (sEVs), through their natural ability to interact with biological membranes and exploit endogenous processing pathways to convey biological information, are quintessential for the delivery of therapeutically relevant compounds, such as microRNAs (miRNAs) and proteins. Here, we used a fluorescently-labelled miRNA to quantify the efficiency of different methods to modulate the cargo o...
High-throughput screening of nanoparticles in drug delivery
Tomé, Inês; Francisco, Vitor; Fernandes, Hugo; Ferreira, LinoThe use of pharmacologically active compounds to manage and treat diseases is of utmost relevance in clinical practice. It is well recognized that spatial-temporal control over the delivery of these biomolecules will greatly impact their pharmacokinetic profile and ultimately their therapeutic effect. Nanoparticles (NPs) prepared from different materials have been tested successfully in the clinic for the deliv...
A high-throughput screening platform to identify nanocarriers for efficient del...
Francisco, Vitor; Rebelo, Catarina; Rodrigues, Artur Filipe; Blersch, Catarina; Fernandes, Hugo; Ferreira, Lino
Native and engineered extracellular vesicles for cardiovascular therapeutics
Abreu, Ricardo Cerqueira de; Fernandes, Hugo; Martins, Paula Costa; Sahoo, Susmita; Emanueli, Costanza; Ferreira, LinoExtracellular vesicles (EVs) are a heterogeneous group of natural particles that are relevant to the treatment of cardiovascular diseases. These endogenous vesicles have certain properties that allow them to survive in the extracellular space, bypass biological barriers and deliver their biologically active molecular cargo to recipient cells. Moreover, EVs can be bioengineered to increase their stability, bioac...
A SOX2 Reporter System Identifies Gastric Cancer Stem-Like Cells Sensitive to M...
Pádua, Diana; Barros, Rita; Amaral, Ana Luísa; Mesquita, Patrícia; Freire, Ana Filipa; Sousa, Mafalda; Maia, André Filipe; Caiado, Inês; Fernandes, HugoGastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of cancer stem cells (CSCs) as seeds of the tumorigenic process makes them a prime therapeutic target. Knowing that the transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like cells in human gastric cancer cell lines using a traceable reporter system based on SOX2/OCT4 acti...
El cuidado al agresor familiar persistente desde la perspectiva de los estudian...
Fernandes, Hugo; Brandão, Maykon Brito; Castilho-Júnior, Roberto Alvarenga de; Hino, Paula; Ohara, Conceição Vieira da SilvaObjective: to analyze the perception of nursing students about the care given to the persistent family aggressor. Method: a descriptive study, with a qualitative approach. Madeleine Leininger’s Theory of Diversity and Universality of Care was used as a theoretical reference. The data collection took place with 37 in the last year of at a public university in Southeast Brazil. We used the technique of Projective...
As interfaces das dimensões da vulnerabilidade face à violência contra a criança
Fernandes, Hugo