31 documents found, page 1 of 4

Extracellular vesicle transfer of lncRNA H19 splice variants to cardiac cells

CC BY-NC-ND 4.0; The authors would like to acknowledge Crioestaminal (www.crioestaminal.pt) for providing samples and other helpful information and Dr. Yingqun Huang for providing the lncRNA H19 plasmid. The authors would further like to acknowledge the funding by the FCT PhD Studentships (SFRH/BD/119187/2016 & SFRH/BD/ 144092/2019), Programa Operacional Competividade e Internacionalização (POCI) na sua compone...

TRAP1 Is Expressed in Human Retinal Pigment Epithelial Cells and Is Required to...

Age-related macular degeneration (AMD) is the leading cause of severe vision loss and blindness in elderly people worldwide. The damage to the retinal pigment epithelium (RPE) triggered by oxidative stress plays a central role in the onset and progression of AMD and results from the excessive accumulation of reactive oxygen species (ROS) produced mainly by mitochondria. Tumor necrosis factor receptor-associated...

Extracellular vesicles enriched with an endothelial cell pro-survival microRNA ...

Endothelial cell (EC) activity is essential for tissue regeneration in several (patho)physiological contexts. However, our capacity to deliver in vivo biomolecules capable of controlling EC fate is relatively limited. Here, we screened a library of microRNA (miR) mimics and identified 25 miRs capable of enhancing the survival of ECs exposed to ischemia-mimicking conditions. In vitro, we showed that miR-425-5p, ...

Influence of nitric oxide and phosphodiesterases during in vitro maturation of ...

Made available in DSpace on 2022-04-29T08:45:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-06-01; This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the solubl...

Effect of follicular fluid supplementation during in vitro maturation on total ...

Made available in DSpace on 2022-04-29T07:14:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01; This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to me...

Exogenous loading of miRNAs into small extracellular vesicles

Small extracellular vesicles (sEVs), through their natural ability to interact with biological membranes and exploit endogenous processing pathways to convey biological information, are quintessential for the delivery of therapeutically relevant compounds, such as microRNAs (miRNAs) and proteins. Here, we used a fluorescently-labelled miRNA to quantify the efficiency of different methods to modulate the cargo o...

High-throughput screening of nanoparticles in drug delivery

The use of pharmacologically active compounds to manage and treat diseases is of utmost relevance in clinical practice. It is well recognized that spatial-temporal control over the delivery of these biomolecules will greatly impact their pharmacokinetic profile and ultimately their therapeutic effect. Nanoparticles (NPs) prepared from different materials have been tested successfully in the clinic for the deliv...

A high-throughput screening platform to identify nanocarriers for efficient del...

Native and engineered extracellular vesicles for cardiovascular therapeutics

Extracellular vesicles (EVs) are a heterogeneous group of natural particles that are relevant to the treatment of cardiovascular diseases. These endogenous vesicles have certain properties that allow them to survive in the extracellular space, bypass biological barriers and deliver their biologically active molecular cargo to recipient cells. Moreover, EVs can be bioengineered to increase their stability, bioac...

A SOX2 Reporter System Identifies Gastric Cancer Stem-Like Cells Sensitive to M...

Gastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of cancer stem cells (CSCs) as seeds of the tumorigenic process makes them a prime therapeutic target. Knowing that the transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like cells in human gastric cancer cell lines using a traceable reporter system based on SOX2/OCT4 acti...