13 documents found, page 1 of 2
Aedes aegypti larvae treated with spinosad produce adults with damaged midgut a...
Fernandes, Kenner Morais; Tomé, Hudson Vaner Ventura; Miranda, Franciane Rosa; Gonçalves, Wagner Gonzaga; Pascini, Tales Vicari; Serrão, José EduardoThe mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here,...
Histochemistry, immunohistochemistry and cytochemistry of the anterior midgut r...
Carneiro, Lenise Silva; Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Zanuncio, José Cola; Serrão, José EduardoThe anterior midgut region of stingless bees is anatomically differentiated with tall and narrow cells, whereas in other social and solitary bees this anatomical gut region is lacking. The objective of the present study was to describe the histochemistry, immunohistochemistry and cytochemistry of the anterior midgut region of the stingless bee Melipona quadrifasciata in comparison with the honey bee Apis mellif...
Spinosad-mediated effects on survival, overall group activity and the midgut of...
Araujo, Renan dos Santos; Lopes, Marcos Pereira; Barbosa, Wagner Faria; Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Martins, Gustavo FerreiraPopulations of stingless bees have declined around the world and pesticides have been indicated as one of the possible causes of this decrease. Spinosad, which is synthesized from the fermentation process produced by the soil actinomycete Saccharopolyspora spinosa, is one of the most used bioinsecticides today. This study aimed to evaluate the possible effects of spinosad (formulation) on survival, general grou...
Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered ...
Paiva, Aline Dias; Fernandes, Kenner Morais; Dias, Roberto Sousa; Rocha, Alípio dos Santos; Oliveira, Leandro Licursi de; Neves, Clóvis AndradeBovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were evaluated. BALB/c mice were divided into three groups: negative control (NC group); mice given purified b...
Proteomic analysis of Aedes aegypti midgut during post-embryonic development an...
Fernandes, Kenner Morais; Magalhães-Júnior, Marcos Jorge de; Baracat-Pereira, Maria Cristina; Martins, Gustavo FerreiraIn this work we analyzed protein expression in the Aedes aegypti midgut during the larval (fourth instar, L4), pupal, and adult stages [including newly emerged (NE), sugar-fed (SF) and blood-fed (BF) females]. Two-dimensional electrophoresis showed 13 spots in the midgut of larvae, 95 in the midgut of pupae, 90 in the midgut of NE, and 76 in the midgut of SF or BF females. In the larval midguts, high serpin exp...
Post-embryonic development of the Malpighian tubules in Apis mellifera (Hymenop...
Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Santana, Weyder Cristiano; Martins, Gustavo Ferreira; Zanuncio, José Cola; Serrão, José EduardoThe honeybee Apis mellifera has ecological and economic importance; however, it experiences a population decline, perhaps due to exposure to toxic compounds, which are excreted by Malpighian tubules. During metamorphosis of A. mellifera, the Malpighian tubules degenerate and are formed de novo. The objective of this work was to verify the cellular events of the Malpighian tubule renewal in the metamorphosis, wh...
Spinosad-mediated effects on the walking abilities, midgut, and Malpighian tubu...
Lopes, Marcos Pereira; Fernandes, Kenner Morais; Tomé, Hudson Vaner Ventura; Gonçalves, Wagner Gonzaga; Miranda, Franciane Rosa; Serrão, José EduardoThe global decline in Apis mellifera colonies is attributed to multiple factors, including pesticides. The bioinsecticide spinosad was initially recognized as safe for non-target organisms; however, its toxicity has been changing this concept. Here, we investigated the survival, behavioral changes, and structural changes in the midgut and Malpighian tubules of A. mellifera treated orally with a spinosad formula...
Post-embryonic changes in the hindgut of honeybee Apis mellifera workers: Morph...
Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Santana, Weyder Cristiano; Martins, Gustavo Ferreira; Zanuncio, José Cola; Serrão, José EduardoIn insects, the hindgut is a homeostatic region of the digestive tract, divided into pylorus, ileum, and rectum, that reabsorbs water, ions, and small molecules produced during hemolymph filtration. The hindgut anatomy in bee larvae is different from that of adult workers. This study reports the morphological changes and cellular events that occur in the hindgut during the metamorphosis of the honeybee Apis mel...
Differential cellular immune response of Galleria mellonella to Actinobacillus ...
Blanco, Luis Andrés Arteaga; Crispim, Josicelli Souza; Fernandes, Kenner Morais; Oliveira, Leandro Licursi de; Pereira, Monalessa FábiaIn the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molec...
Schinus terebinthifolius leaf extract causes midgut damage, interfering with su...
Procópio, Thamara Figueiredo; Fernandes, Kenner Morais; Pontual, Emmanuel Viana; Ximenes, Rafael Matos; Oliveira, Aline Rafaella Cardoso deIn this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3–1.35%, w/v) for 8 days, in presence or ab...