9 documents found, page 1 of 1
Peptidomics Unveils Distinct Acetylation Patterns of Histone and Annexin A1 in ...
Coelho, M; Capela, J; Mendes, VM; Pacheco, J; Fernandes, MS; Amendoeira, I; Jones, JG; Raposo, L; Manadas, BThyroid cancer is a common malignancy of the endocrine system. Nodules are routinely evaluated for malignancy risk by fine needle aspiration biopsy (FNAB), and in cases such as follicular lesions, differential diagnosis between benign and malignant nodules is highly uncertain. Therefore, the discovery of new biomarkers for this disease could be helpful in improving diagnostic accuracy. Thyroid nodule biopsies w...
Proteomics Reveals mRNA Regulation and the Action of Annexins in Thyroid Cancer
Coelho, M; Capela, J; Anjo, SI; Pacheco, J; Fernandes, MS; Amendoeira, I; Jones, JG; Raposo, L; Manadas, BDifferentiated thyroid cancer is the most common malignancy of the endocrine system. Although most thyroid nodules are benign, given the high incidence of thyroid nodules in the population, it is important to understand the differences between benign and malignant thyroid cancer and the molecular alterations associated with malignancy to improve detection and signal potential diagnostic, prognostic, and therape...
TERTp mutations and p53 expression in head and neck cutaneous basal cell carcin...
Castanheira, A; Vieira, MJ; Pinto, M; Dias, C; Prada, L; Macedo, S; Fernandes, MS; Vieira, F; Soares, P; Mota, AV; Lopes, JM; Boaventura, PCutaneous basal cell carcinoma (cBCC) is an economic burden to health services, due to its great morbidity and increasing incidence in old people. Infiltrative cBCCs and cBCCs with micronodular pattern are considered as more aggressive. The role of p53 expression and TERTp mutation on cBCC behavior remains to be clarified. We aimed to assess TERTp mutations and p53 expression in relation to the cBCC histologica...
A machine learning approach for single cell interphase cell cycle staging
Narotamo, H; Fernandes, MS; Moreira, AM; Melo, S; Seruca, R; Silveira, M; Sanches, JMThe cell nucleus is a tightly regulated organelle and its architectural structure is dynamically orchestrated to maintain normal cell function. Indeed, fluctuations in nuclear size and shape are known to occur during the cell cycle and alterations in nuclear morphology are also hallmarks of many diseases including cancer. Regrettably, automated reliable tools for cell cycle staging at single cell level using in...
Prognostic significance of RAS mutations and P53 expression in cutaneous squamo...
Campos, MA; Macedo, S; Fernandes, MS; Pestana, A; Pardal, J; Batista, R; Vinagre, J; Sanches, A; Baptista, A; Lopes, JM; Soares, PTP53 is considered the most commonly-altered gene in cutaneous squamous cell carcinoma (cSCC). Conversely, RAS mutations have been reported in a low percentage of cSCC. The objective of our study was to evaluate the frequency of p53 expression and RAS mutations in cSCC and correlate them with clinicopathological features and patient outcome. We performed immunohistochemistry for p53 and genetic profiling for RA...
Hereditary gastric and breast cancer syndromes related to CDH1 germline mutatio...
Corso, G; Montagna, G; Figueiredo, J; Vecchia, CL; Romario, UF; Fernandes, MS; Seixas, S; Roviello, F; Trovato, C; Guerini-Rocco, E; Fusco, NE-cadherin (CDH1 gene) germline mutations are associated with the development of diffuse gastric cancer in the context of the so-called hereditary diffuse gastric syndrome, and with an inherited predisposition of lobular breast carcinoma. In 2019, the international gastric cancer linkage consortium revised the clinical criteria and established guidelines for the genetic screening of CDH1 germline syndromes. Nev...
The Extracellular Matrix: An Accomplice in Gastric Cancer Development and Progr...
Moreira, AM; Pereira, J; Melo, S; Fernandes, MS; Carneiro, P; Seruca, R; Figueiredo, JThe extracellular matrix (ECM) is a dynamic and highly organized tissue structure, providing support and maintaining normal epithelial architecture. In the last decade, increasing evidence has emerged demonstrating that alterations in ECM composition and assembly strongly affect cellular function and behavior. Even though the detailed mechanisms underlying cell-ECM crosstalk are yet to unravel, it is well estab...
S100P is a molecular determinant of E-cadherin function in gastric cancer
Carneiro, P; Moreira, AM; Figueiredo, J; Barros, R; Oliveira, P; Fernandes, MS; Ferro, A; Almeida, R; Oliveira, C; Carneiro, F; Schmitt, F; Paredes, JBackground: E-cadherin has been awarded a key role in the aetiology of both sporadic and hereditary forms of gastric cancer. In this study, we aimed to identify molecular interactors that influence the expression and function of E-cadherin associated to cancer. Methods: A data mining approach was used to predict stomach-specific candidate genes, uncovering S100P as a key candidate. The role of S100P was evaluat...
Geometric compensation applied to image analysis of cell populations with morph...
Figueiredo, J; Rodrigues, I; Ribeiro, J; Fernandes, MS; Melo, S; Sousa, B; Paredes, J; Seruca, R; Sanches, JImmunofluorescence is the gold standard technique to determine the level and spatial distribution of fluorescent-tagged molecules. However, quantitative analysis of fluorescence microscopy images faces crucial challenges such as morphologic variability within cells. In this work, we developed an analytical strategy to deal with cell shape and size variability that is based on an elastic geometric alignment algo...