19 documents found, page 1 of 2
Validation of app and phone versions of the Control of Allergic Rhinitis and As...
Jácome, C.; Pereira, R.; Almeida, R.; Amaral, Rita; Correia, M. A.; Mendes, S.; Vieira-Marques, P.; Ferreira, J. A.; Lopes, I.; Gomes, J.; Vidal, C.Assessment of asthma control at every opportunity is recommended. Control of Allergic Rhinitis and Asthma Test (CARAT) is a patient reported outcome measure commonly used to assess asthma control in clinical practice.It includes ten questions answered in a 4 point Like rt scale that address upper and lower airway symptoms, sleep interference, limitation of activities , and the need to increase medication over a...
Efeito da aplicação ao solo de água-ruça, bagaço de azeitona e um composto sobr...
Sempiterno, C. M.; Fernandes, R. M.A aplicação ao solo, dos resíduos dos lagares de azeite, em bruto ou após um processo de compostagem, poderá assegurar um destino final para estes resíduos e ao mesmo tempo permitir a valorização agrícola de alguns dos seus componentes. Neste estudo, avaliaram-se os efeitos da aplicação ao solo de uma água-ruça (AR) e de um bagaço (B) provenientes de um lagar com sistema de extracção de três fases, bem como d...
Ingestive behavior of young grazing Nellore bulls supplemented with sources of ...
Mota, V. A. C. [UNESP]; Fernandes, R. M.; Nascimento, C. F. [UNESP]; Issa, H. A. S. [UNESP]; Holder, V. B.; Pettigrew, J. E.; Resende, F. D.Made available in DSpace on 2018-11-26T15:45:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-08-01; Sao Paulo Res Fdn FAPESP, Sao Paulo, Brazil; UNESP FCAV, Jaboticabal, Brazil; UNESP Univ Estadual Paulista, Jaboticabal, Brazil; UNIFEB, Barretos, SP, Brazil; Alltech Inc, Nicholasville, KY USA; Univ Illinois, Urbana, IL 61801 USA; Agencia Paulista Tecnol Agronegocios APTA, Colina, Brazil; UNESP FCAV, J...
Validation of a fluorescence in situ hybridization method using peptide nucleic...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisHere, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori cla...
Probe4pylori: a new kit for the rapid detection of H. pylori and associated cla...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Oleastro, Mónica; Carneiro, Fátima; Brandão, Catarina; Nunes, Pedro Pimentel; Ribeiro, Mário DinisIn this study an evaluation of Probe4Pylori, a previously established PNA-FISH diagnostic test for H. pylori clarithromycin resistance in paraffin embedded gastric biopsies was performed and compared against culture followed by E-test and PCR. The Probe4Pylori showed very promising results with values of sensitivity (80%) and specificity (approximately 90%) for the patients harboring clarithromycin-resistant H....
PNA-FISH as a new diagnostic method for the determination of clarithromycin res...
Fernandes, R. M.; Cerqueira, L.; Ferreira, Rui M.; Carneiro, Fátima; Ribeiro, Mário Dinis; Figueiredo, C.; Keevil, C. W.; Azevedo, N. F.; Vieira, M. J.Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent ...
Application of fluorescence in situ hybridisation using peptide nucleic acid pr...
Cerqueira, L.; Fernandes, R. M.; Ferreira, Rui M.; Carneiro, F.; Ribeiro, M. Dinis; Figueiredo, C.; Azevedo, N. F.; Keevil, C. W.; Vieira, M. J.Microorganisms are responsible for several infectious diseases that can cause severe problems to patients and their treatment success is seriously correlated with the fast detection of the infectious agent. Some of the standard methods used, such as culturing methods are fastidious and time-consuming and do not give any information about the antibiotic resistance profile. Therefore, molecular methods have been ...
Fluorescence in situ hybridization method using a peptide nucleic acid probe fo...
Almeida, Carina; Azevedo, N. F.; Fernandes, R. M.; Keevil, C. W.; Vieira, M. J.A fluorescence in situ hybridization (FISH) method for the rapid detection of Salmonella spp. using a novel peptide nucleic acid (PNA) probe was developed. Specificity and sensitivity probe matching theoretical estimates were both 100%. The PNA FISH method was optimized, and laboratory testing on representative strains from the Salmonella genus subspecies and several related bacterial species, confirmed the pre...
Oral Candida carriage of patients attending a dental clinic in Braga, Portugal
Martins, Margarida Isabel Barros Coelho; Henriques, Mariana; Ribeiro, A. P.; Fernandes, R. M.; Gonçalves, Vânia; Seabra, A.; Azeredo, JoanaThe ability of the Candida species to colonize surfaces can be considered as a risk factor for oral infection. Aims The aim of this work was to establish oral Candida carriage in patients attending a dental clinic in Braga, Portugal. Methods A total of 97 patients were analysed. Swab samples were collected, and directly cultured onto CHROMagar Candida. Representative yeasts were identified by polymerase chain r...
Characterization of multispecies biofilms by peptide nucleic acid fluorescence ...
Almeida, Carina; Vieira, M. J.; Keevil, C. W.; Fernandes, R. M.; Azevedo, N. F.Our current understanding of biofilms in the environment and in health indicates that these structures are typically composed of many different microbial species. However, the lack of reliable techniques for the quantification, visualization and discrimination of each population has meant that studies assessing multi-species interactions between sessile microorganisms are scarce and low-throughput. Employing no...