34 documents found, page 1 of 4
Cutibacterium acnes dysbiosis: Alternative therapeutics for clinical application
Sá, Sara; Fernandes, Ruben; Gestoso, Álvaro; Macedo, José Mário; Martins-Mendes, Daniela; Pereira, Ana Cláudia; Baylina, PilarCutibacterium acnes (C. acnes) is a Gram-positive anaerobic facultative bacterium that is part of the human skin commensal microbiome. It colonizes various regions of the body, including the face, back, and chest. While typically a harmless commensal, under certain conditions, C. acnes can become pathogenic, leading to or promoting conditions such as acne vulgaris (AV), post-surgical infections, prostate cancer...
The influence of adipocyte secretome on selected metabolic fingerprints of brea...
Luís, Carla; Guerra-Carvalho, Bárbara; Braga, Patrícia C.; Guedes, Carla; Patrício, Emília; Alves, Marco G.; Fernandes, Ruben; Soares, RaquelMolecular subtype (MS) is one of the most used classifications of breast cancer (BC). Four MSs are widely accepted according to receptor expression of estrogen, progesterone, and HER2. The impact of adipose tissue on BC MS metabolic impairment is still unclear. The present work aims to elucidate the metabolic alterations in breast cancer cell lines representing different MSs subjected to adipocyte associated fa...
Virulence-linked mutations in rubredoxin reductase and glutaredoxin: impact on ...
Sá, Sara; Silva, Carina; Dias, Maria Clara; Veiga, Marlene; Lopes, Sofia; Fernandes, Ruben; Rocha, Ana Catarina; Pais, Patrick J.; Oliveira, MarcoPseudomonas aeruginosa (PAO1) is an opportunistic pathogen, lethal in immunocompromised individuals. The clinical management of PAO1 infections still depends deeply on antibiotic therapy. However, this therapy has been alarmingly overpowered by growing bacterial resistance mechanisms over the years. One of these bacterial mechanisms is quorum sensing (QS). QS is involved in the production of biofilm, rhamnolipi...
New CTX‐M group conferring β‐lactam resistance: a compendium of phylogenetic in...
Mendonça, Jacinta; Guedes, Carla; Silva, Carina; Sá, Sara; Oliveira, Marco; Accioly, Gustavo; Baylina, Pilar; Barata, Pedro; Pereira, CláudiaThe production of extended‐spectrum β‐lactamases (ESBLs) is the main defense mechanism found in Gram negative bacteria. Among all the ESBLs, the CTX‐M enzymes appear as the most efficient in terms of dissemination in different epidemiological contexts. CTX‐M enzymes exhibit a striking plas-ticity, with a large number of allelic variants distributed in several sublineages, which can be associated with functional...
The history and applications of phage therapy in pseudomonas aeruginosa
Silva, Carina; Sá, Sara; Guedes, Carla; Oliveira, Carla; Lima, Cláudio; Oliveira, Marco; Mendes, João; Novais, Gonçalo; Baylina, Pilar; Fernandes, RubenThe Pseudomonas aeruginosa is one of the bacteria that cause serious infections due to resistance to many antibiotics can be fatal in severe cases. Antimicrobial resistance is a global public health concern. To solve this problem, interest in phage therapy has revived; some studies are being developed to try to prove the effectiveness of this therapy. Thus, in this opinion article, several historical aspects ar...
Cannabidiol and terpene formulation reducing SARS-CoV-2 infectivity tackling a ...
Santos, Susana; Barata, Pedro; Charmier, Adilia; Lehmann, Inês; Rodrigues, Suzilaine; Melosini, Matteo M.; Pais, Patrick J.; Sousa, André P.In late 2019, COVID-19 emerged in Wuhan, China. Currently, it is an ongoing global health threat stressing the need for therapeutic compounds. Linking the virus life cycle and its interaction with cell receptors and internal cellular machinery is key to developing therapies based on the control of infectivity and inflammation. In this framework, we evaluate the combination of cannabidiol (CBD), as an anti-infla...
New CTX-M group conferring β-Lactam resistance: A compendium of phylogenetic in...
Mendonça, Jacinta; Guedes, Carla; Silva, Carina; Sá, Sara; Oliveira, Marco; Accioly, Gustavo; Baylina, Pilar; Barata, Pedro; Pereira, CláudiaThe production of extended-spectrum β-lactamases (ESBLs) is the main defense mechanism found in Gram negative bacteria. Among all the ESBLs, the CTX-M enzymes appear as the most efficient in terms of dissemination in different epidemiological contexts. CTX-M enzymes exhibit a striking plasticity, with a large number of allelic variants distributed in several sublineages, which can be associated with functional ...
New Quantum-Dot-Based Fluorescent Immunosensor for Cancer Biomarker Detection
Sousa, Mariana P.; Piloto, Ana Margarida L.; Pereira, Ana Cláudia; Schmitt, Fernando; Fernandes, Ruben; Moreira, Felismina T. C.Cancer antigen 15-3 (CA 15-3) is a biomarker for breast cancer used to monitor response to treatments and disease recurrence. The present work demonstrates the preparation and application of a fluorescent biosensor for ultrasensitive detection of the cancer antigen CA 15-3 protein tumor marker using mercaptopropionic-acid-functionalized cadmium telluride (CdTe@MPA) quantum dots (QDs) conjugated with CA 15-3 ant...
New quantum-dot-based fluorescent immunosensor for cancer biomarker detection
Sousa, Mariana; Piloto, Margarida; Pereira, Ana Cláudia; Schmitt, Fernando C.; Fernandes, Ruben; Moreira, FelisminaCancer antigen 15-3 (CA 15-3) is a biomarker for breast cancer used to monitor response to treatments and disease recurrence. The present work demonstrates the preparation and application of a fluorescent biosensor for ultrasensitive detection of the cancer antigen CA 15-3 protein tumor marker using mercaptopropionic-acid-functionalized cadmium telluride (CdTe
Plastic Antibody of Polypyrrole/Multiwall Carbon Nanotubes on Screen-Printed El...
Gomes, Rui S.; Gomez-Rodríguez, Blanca Azucena; Fernandes, Ruben; Sales, M. Goreti F.; Moreira, Felismina T C; Dutra, Rosa F.This work reports the design of a novel plastic antibody for cystatin C (Cys-C), an acute kidney injury biomarker, and its application in point-of-care (PoC) testing. The synthetic antibody was obtained by tailoring a molecularly imprinted polymer (MIP) on a carbon screen-printed electrode (SPE). The MIP was obtained by electropolymerizing pyrrole (Py) with carboxylated Py (Py-COOH) in the presence of Cys-C and...