10 documents found, page 1 of 1
Sample preparation and chromatographic methods for the determination of protein...
Fernandes, Sara R.; Meireles, Andreia N.; Marques, Sara S.; Silva, Luís; Barreiros, Luisa; Sampaio-Maia, Benedita; Miro, Manuel; Segundo, Marcela A.Protein-bound uremic retention solutes, such as indole-3-acetic acid, indoxyl sulfate, p-cresol and p-cresol sulfate, are associated with the development of several pathologies, namely renal, cardiovascular, and bone toxicities, due to their potential accumulation in the human body, thus requiring analytical methods for monitoring and evaluation. The present review addresses conventional and advanced sample tre...
Acetonitrile Adducts of Tranexamic Acid as Sensitive Ions for Quantification at...
Silva, Eduarda M P; Barreiros, Luisa; Fernandes, Sara R.; Sa, Paula; Prates Ramalho, João P.; Segundo, MarcelaThe quantitative analysis of pharmaceuticals in biomatrices by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is often hampered by adduct formation. The use of the molecular ion resulting from solvent adducts for quantification is uncommon, even if formed in high abundance. In this work, we propose the use of a protonated acetonitrile adduct for the quantitati...
Acetonitrile adducts of tranexamic acid as sensitive ions for quantification at...
Silva, Eduarda M. P.; Barreiros, Luísa; Fernandes, Sara R.; Sá, Paula; Ramalho, João P. Prates; Segundo, Marcela A.The quantitative analysis of pharmaceuticals in biomatrices by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is often hampered by adduct formation. The use of the molecular ion resulting from solvent adducts for quantification is uncommon, even if formed in high abundance. In this work, we propose the use of a protonated acetonitrile adduct for the quantitati...
Monitoring tranexamic acid in human urine by automatic solid-phase extraction c...
Barreiros, Luisa; Barreiros, Luisa; Sá, P.; Miró, M.; Segundo, Marcela A.; Fernandes, Sara R.; Fernandes, SaraTranexamic acid (TXA) is an important antifibrinolytic agent in the treatment of different haemorrhagic conditions.1,2 However, the information about pharmacokinetics and pharmacodynamics is scarce. Therefore, the development of analytical methods for the quantification of TXA in different types of biological samples is required, because this information will be relevant in the establishment of adequate doses. ...
Determination of tranexamic acid in human plasma by UHPLC coupled with tandem m...
Barreiros, Luisa; Amoreira, Júlia L.; Machado, Sandia; Fernandes, Sara R.; Silva, Eduarda M.P.; Sá, Paula; Kietaibl, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA) is an antifibrinolytic drug, with the ability to inhibit lysine binding at plasminogen receptors, used in adult trauma patients with on-going or at risk of significant haemorrhage. To understand the pharmacokinetics and pharmacodynamics of this drug in variable age groups undergoing surgeries with high blood loss, effective methods for determination of TXA in biological samples at sub-μg m...
Analytical strategies based on tandem mass spectrometry detection for quantific...
Barreiros, Luisa; Fernandes, Sara R.; Machado, Sandia; Silva, Eduarda M. P.; Segundo, Marcela A.Fast and accurate analysis, providing reliable results at trace concentration levels, is a current demand of the modern world. This pressure is justifiable in limit situations but also in our daily life, for instance when waiting for a diagnosis based on lab results in a hospital or when wondering about the quality of water running from our taps. During the last years, tandem mass spectrometry (MS/MS) based tec...
Chemistry, bioactivities, extraction and analysis of azadirachtin: state-of-the...
Fernandes, Sara R.; Barreiros, Luisa; Oliveira, Rita F.; Cruz, Agostinho; Prudêncio, Cristina; Oliveira, Ana Isabel; Pinho, Cláudia; Santos, NunoAzadirachta indica A. Juss. (Neem) is an Indian tree recognized for its activity as pesticide, as well as several pharmacological properties. Among the various compounds already isolated and studied from Neem tree, azadirachtin (AZA) was identified as the main bioactive compound. Azadirachtin can be found at different parts of the Neem plant but assumes its maximum concentration at the seed level. This compound...
Bioacessibility of zinc in pet food determined by a dynamic leaching method
Fernandes, Sara R.; Pereira, Ana Margarida; Matos, Elisabete; Castanheira, Francisco; Baptista, Cláudia S.; Cabrita, Ana Rita J.; Segundo, Marcela A.In dynamic leaching methods, portions of extractant reagents are continuously provided to the solid sample contained in flow-through microcolumns or chambers, enabling the renewal of extracting fluid and avoiding saturation effects from fluid stagnation. These methods are also suitable for fast measurements in real time with small extract manipulation, especially when coupled online with suitable detectors [1]....
Quantification of tranexamic acid in human plasma: development and validation o...
Barreiros, Luisa; Amoreira, Júlia L.; Machado, Sandia; Fernandes, Sara R.; Silva, Eduarda M. P.; Sá, Paula; Kietaibl, Sibylle; Segundo, Marcela A.Tranexamic acid (TXA), an antifibrinolytic drug with the ability to inhibit lysine binding at plasminogen receptors, can be used in different settings such as trauma, cardiac surgery, major orthopedic surgery, obstetric when perioperative bleeding is concerned [1]. Effective methods for determination of TXA in biological samples are still required to understand the pharmacokinetics and pharmacodynamics of this ...
Simultaneous determination of dapsone and clofazimine in nanoformulations by HPLC
Fernandes, Sara R.; Fernandes, Sara; Chaves, Luíse L.; Lima, Sofia A. C.; Silva, Eduarda M. P.; Barreiros, Luísa; Reis, Salette; Segundo, Marcela A.The multidrug therapy with dapsone (DAP) and clofazimine (CLZ) is known as an effective treatment against Mycobacterium leprae. However, the low bioavailability and non-specific distribution can reduce therapy efficacy and produce side effects. The use of nanotechnological approaches was explored as a promising carrier for delivery enhancement of these drugs. Therefore, a simple and precise highperformance liqu...